| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Cell Biology, Institute of Anatomy, University of Aarhus, DK-8000; 2 Department of Clinical Physiology, Aarhus University Hospital, and Institute of Experimental Clinical Research, DK-8200 Aarhus N, Denmark; and 3 Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892
The role of AVP-V2 receptor (AVP-V2R)-dependent regulation of aquaporin-2 (AQP2) expression was evaluated in vasopressin-deficient Brattleboro (BB) rats. AQP2 levels were relatively high in BB rats (52 ± 8% of levels in Wistar rats), and treatment with the AVP-V2R antagonist SR-121463A (0.8 mg/day) for 48 h was associated with 1) increased urine output (170 ± 9%), 2), reduced AQP2 protein levels (42 ± 10% in whole kidney and 53 ± 8% in inner medulla), and 3) reduced AQP2 mRNA levels (36 ± 7%). In addition, the levels of AQP2 phosphorylated in the protein kinase A (PKA) consensus site (Ser256 of AQP2) was reduced to 3 ± 1% of control levels. Lithium (Li) treatment of BB rats for 1 mo, known to reduce adenylyl cyclase (AC) activity, downregulated AQP2 protein levels (15 ± 6%) and increased urine output (220%). Downregulation of AQP2 expression in response to SR-121463A or Li treatment indicates that AQP2 expression in BB rats depends in part on activation of AVP-V2Rs and that the signaling cascade(s) involves AC and hence cAMP. Complete water restriction of BB rats produced only a small increase in AQP2 mRNA (235 ± 33%) and AQP2 protein (156 ± 22%) levels. Immunoelectron microscopy confirmed the increase in AQP2 abundance but revealed no change in AQP2 apical plasma membrane labeling in response to thirsting. In conclusion, the expression and phosphorylation of AQP2 in BB rats are in part dependent on AVP-V2R signaling, and AVP-V2-mediated regulation of AQP2 trafficking and expression is effectively decoupled in BB rats, indicating differences in AVP-V2R-mediated regulation of AQP2 trafficking and expression.
arginine vasopressin; aquaporin; collecting duct; urinary concentrating mechanism; water transport
This article has been cited by other articles:
|
|
 |
|
  M. Boone, M. Kortenoeven, J. H. Robben, and P. M. T. Deen Effect of the cGMP pathway on AQP2 expression and translocation: potential implications for nephrogenic diabetes insipidus Nephrol. Dial. Transplant., August 8, 2009; (2009) gfp409v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Boulkroun, D. Ruffieux-Daidie, J.-J. Vitagliano, O. Poirot, R.-P. Charles, D. Lagnaz, D. Firsov, S. Kellenberger, and O. Staub Vasopressin-inducible ubiquitin-specific protease 10 increases ENaC cell surface expression by deubiquitylating and stabilizing sorting nexin 3 Am J Physiol Renal Physiol, October 1, 2008; 295(4): F889 - F900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Mutig, A. Paliege, T. Kahl, T. Jons, W. Muller-Esterl, and S. Bachmann Vasopressin V2 receptor expression along rat, mouse, and human renal epithelia with focus on TAL Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1166 - F1177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-H. Han, U-Y. Lee, Y.-S. Jang, Y. M. Cho, Y. M. Jang, I.-A Hwang, J. Y. Ghee, S.-W. Lim, W.-Y. Kim, C. W. Yang, et al. Differential regulation of B/K protein expression in proximal and distal tubules of rat kidneys with ischemia-reperfusion injury Am J Physiol Renal Physiol, January 1, 2007; 292(1): F100 - F106. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Russo, M. McKee, and D. Brown Methyl-beta-cyclodextrin induces vasopressin-independent apical accumulation of aquaporin-2 in the isolated, perfused rat kidney Am J Physiol Renal Physiol, July 1, 2006; 291(1): F246 - F253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Li, W. Wang, S. N. Summer, M. A. Cadnapaphornchai, S. Falk, F. Umenishi, and R. W. Schrier Hyperosmolality In Vivo Upregulates Aquaporin 2 Water Channel and Na-K-2Cl Co-Transporter in Brattleboro Rats J. Am. Soc. Nephrol., June 1, 2006; 17(6): 1657 - 1664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Wang, C. Li, S. N. Summer, S. Falk, M. A. Cadnapaphornchai, Y.-C. Chen, and R. W. Schrier Molecular analysis of impaired urinary diluting capacity in glucocorticoid deficiency Am J Physiol Renal Physiol, May 1, 2006; 290(5): F1135 - F1142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Li, S. Shaw, E.-J. Kamsteeg, A. Vandewalle, and P. M.T. Deen Development of Lithium-Induced Nephrogenic Diabetes Insipidus Is Dissociated from Adenylyl Cyclase Activity J. Am. Soc. Nephrol., April 1, 2006; 17(4): 1063 - 1072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Nielsen, T.-H. Kwon, J. Praetorius, J. Frokiaer, M. A. Knepper, and S. Nielsen Aldosterone increases urine production and decreases apical AQP2 expression in rats with diabetes insipidus Am J Physiol Renal Physiol, February 1, 2006; 290(2): F438 - F449. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Hasler, S. Nielsen, E. Feraille, and P.-Y. Martin Posttranscriptional control of aquaporin-2 abundance by vasopressin in renal collecting duct principal cells Am J Physiol Renal Physiol, January 1, 2006; 290(1): F177 - F187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-H. Kwon, J. Nielsen, M. A. Knepper, J. Frokiaer, and S. Nielsen Angiotensin II AT1 receptor blockade decreases vasopressin-induced water reabsorption and AQP2 levels in NaCl-restricted rats Am J Physiol Renal Physiol, April 1, 2005; 288(4): F673 - F684. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-H. Kim, J. W. Lee, Y. K. Oh, H. R. Chang, K. W. Joo, K. Y. Na, J.-H. Earm, M. A. Knepper, and J. S. Han Antidiuretic Effect of Hydrochlorothiazide in Lithium-Induced Nephrogenic Diabetes Insipidus Is Associated with Upregulation of Aquaporin-2, Na-Cl Co-transporter, and Epithelial Sodium Channel J. Am. Soc. Nephrol., November 1, 2004; 15(11): 2836 - 2843. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Kim, J. M. Sands, and J. D. Klein Role of vasopressin in diabetes mellitus-induced changes in medullary transport proteins involved in urine concentration in Brattleboro rats Am J Physiol Renal Physiol, April 1, 2004; 286(4): F760 - F766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, W. Wang, M. A. Knepper, S. Nielsen, and J. Frokiar Downregulation of renal aquaporins in response to unilateral ureteral obstruction Am J Physiol Renal Physiol, May 1, 2003; 284(5): F1066 - F1079. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. M. Christensen, W. Wang, J. Frokiar, and S. Nielsen Axial heterogeneity in basolateral AQP2 localization in rat kidney: effect of vasopressin Am J Physiol Renal Physiol, April 1, 2003; 284(4): F701 - F717. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nielsen, J. Frokiar, D. Marples, T.-H. Kwon, P. Agre, and M. A. Knepper Aquaporins in the Kidney: From Molecules to Medicine Physiol Rev, January 1, 2002; 82(1): 205 - 244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Shukla, H. Hager, T. J. Corydon, A. J. Bean, R. Dahl, Z. Vajda, H. Li, H. J. Hoffmann, and S. Nielsen SNAP-25-associated Hrs-2 protein colocalizes with AQP2 in rat kidney collecting duct principal cells Am J Physiol Renal Physiol, September 1, 2001; 281(3): F546 - F556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Robert-Nicoud, M. Flahaut, J.-M. Elalouf, M. Nicod, M. Salinas, M. Bens, A. Doucet, P. Wincker, F. Artiguenave, J.-D. Horisberger, et al. Transcriptome of a mouse kidney cortical collecting duct cell line: Effects of aldosterone and vasopressin PNAS, February 15, 2001; (2001) 51603198. [Abstract] [Full Text]
|
|
|
|
|
|
M. Robert-Nicoud, M. Flahaut, J.-M. Elalouf, M. Nicod, M. Salinas, M. Bens, A. Doucet, P. Wincker, F. Artiguenave, J.-D. Horisberger, et al. Transcriptome of a mouse kidney cortical collecting duct cell line: Effects of aldosterone and vasopressin PNAS, February 27, 2001; 98(5): 2712 - 2716. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
