AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 279: F459-F467, 2000;
0363-6127/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (33)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kim, G.-H.
Right arrow Articles by Knepper, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, G.-H.
Right arrow Articles by Knepper, M. A.
Vol. 279, Issue 3, F459-F467, September 2000

Long-term regulation of renal Na-dependent cotransporters and ENaC: response to altered acid-base intake

Gheun-Ho Kim1, Stephen W. Martin2, Patricia Fernández-Llama1, Shyama Masilamani1, Randall K. Packer2, and Mark A. Knepper1

1 Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892-1603; and 2 Department of Biological Sciences, George Washington University, Washington, District of Columbia 20052

Increased systemic acid intake is associated with an increase in apical Na/H exchange in the renal proximal tubule mediated by the type 3 Na/H exchanger (NHE3). Because NHE3 mediates both proton secretion and Na absorption, increased NHE3 activity could inappropriately perturb Na balance unless there are compensatory changes in Na handling. In this study, we use semiquantitative immunoblotting of rat kidneys to investigate whether acid loading is associated with compensatory decreases in the abundance of renal tubule Na transporters other than NHE3. Long-term (i.e., 7-day) acid loading with NH4Cl produced large decreases in the abundances of the thiazide-sensitive Na-Cl cotransporter (TSC/NCC) of the distal convoluted tubule and both the beta - and gamma -subunits of the amiloride-sensitive epithelial Na channel (ENaC) of the collecting duct. In addition, the renal cortical abundance of the proximal type 2 Na-dependent phosphate transporter (NaPi-2) was markedly decreased. In contrast, abundances of the bumetanide-sensitive Na-K-2Cl cotransporter of the thick ascending limb and the alpha -subunit of ENaC were unchanged. A similar profile of changes was seen with short-term (16-h) acid loading. Long-term (7-day) base loading with NaHCO3 resulted in the opposite pattern of response with marked increases in the abundances of the beta - and gamma -subunits of ENaC and NaPi-2. These adaptations may play critical roles in the maintenance in Na balance when changes in acid-base balance occur.

sodium-proton exchange; sodium-potassium-2 chloride cotransport; distal convoluted tubule; collecting duct; amiloride-sensitive epithelial sodium channel


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
Y. H. Kim, V. Pech, K. B. Spencer, W. H. Beierwaltes, L. A. Everett, E. D. Green, W. Shin, J. W. Verlander, R. L. Sutliff, and S. M. Wall
Reduced ENaC protein abundance contributes to the lower blood pressure observed in pendrin-null mice
Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1314 - F1324.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. W. Verlander
The thiazide-sensitive NaCl cotransporter: a new target for acute regulation of salt and water transport by angiotensin II
Am J Physiol Renal Physiol, September 1, 2007; 293(3): F660 - F661.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. de Seigneux, H. Malte, H. Dimke, J. Frokiaer, S. Nielsen, and S. Frische
Renal compensation to chronic hypoxic hypercapnia: downregulation of pendrin and adaptation of the proximal tubule
Am J Physiol Renal Physiol, April 1, 2007; 292(4): F1256 - F1266.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. M. Jensen, C. Li, H. A. Praetorius, R. Norregaard, S. Frische, M. A. Knepper, S. Nielsen, and J. Frokiaer
Angiotensin II mediates downregulation of aquaporin water channels and key renal sodium transporters in response to urinary tract obstruction
Am J Physiol Renal Physiol, November 1, 2006; 291(5): F1021 - F1032.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. Faroqui, S. Sheriff, and H. Amlal
Metabolic acidosis has dual effects on sodium handling by rat kidney
Am J Physiol Renal Physiol, August 1, 2006; 291(2): F322 - F331.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. Tiwari, R. K. Packer, X. Hu, Y. Sugimura, J. G. Verbalis, and C. A. Ecelbarger
Increased renal {alpha}-ENaC and NCC abundance and elevated blood pressure are independent of hyperaldosteronism in vasopressin escape
Am J Physiol Renal Physiol, July 1, 2006; 291(1): F49 - F57.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
G. Gamba
Molecular Physiology and Pathophysiology of Electroneutral Cation-Chloride Cotransporters
Physiol Rev, April 1, 2005; 85(2): 423 - 493.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. B. Wade, J. Liu, R. A. Coleman, R. Cunningham, D. A. Steplock, W. Lee-Kwon, T. L. Pallone, S. Shenolikar, and E. J. Weinman
Localization and interaction of NHERF isoforms in the renal proximal tubule of the mouse
Am J Physiol Cell Physiol, December 1, 2003; 285(6): C1494 - C1503.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Song, M. A. Knepper, J. G. Verbalis, and C. A. Ecelbarger
Increased renal ENaC subunit and sodium transporter abundances in streptozotocin-induced type 1 diabetes
Am J Physiol Renal Physiol, December 1, 2003; 285(6): F1125 - F1137.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Renal Physiol.Home page
O. A. Weisz and J. P. Johnson
Noncoordinate regulation of ENaC: paradigm lost?
Am J Physiol Renal Physiol, November 1, 2003; 285(5): F833 - F842.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Lebowitz, B. An, R. S. Edinger, M. L. Zeidel, and J. P. Johnson
Effect of altered Na+ entry on expression of apical and basolateral transport proteins in A6 epithelia
Am J Physiol Renal Physiol, September 1, 2003; 285(3): F524 - F531.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
K. Y. Na, Y. K. Oh, J. S. Han, K. W. Joo, J. S. Lee, J.-H. Earm, M. A. Knepper, and G.-H. Kim
Upregulation of Na+ transporter abundances in response to chronic thiazide or loop diuretic treatment in rats
Am J Physiol Renal Physiol, January 1, 2003; 284(1): F133 - F143.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. Masilamani, X. Wang, G.-H. Kim, H. Brooks, J. Nielsen, S. Nielsen, K. Nakamura, J. B. Stokes, and M. A. Knepper
Time course of renal Na-K-ATPase, NHE3, NKCC2, NCC, and ENaC abundance changes with dietary NaCl restriction
Am J Physiol Renal Physiol, October 1, 2002; 283(4): F648 - F657.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Shenolikar, J. W. Voltz, C. M. Minkoff, J. B. Wade, and E. J. Weinman
Targeted disruption of the mouse NHERF-1 gene promotes internalization of proximal tubule sodium-phosphate cotransporter type IIa and renal phosphate wasting
PNAS, August 20, 2002; 99(17): 11470 - 11475.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Manning, K. Beutler, M. A. Knepper, and V. M. Vehaskari
Upregulation of renal BSC1 and TSC in prenatally programmed hypertension
Am J Physiol Renal Physiol, July 1, 2002; 283(1): F202 - F206.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
N. Takahashi, H. L. Brooks, J. B. Wade, W. Liu, Y. Kondo, S. Ito, M. A. Knepper, and O. Smithies
Posttranscriptional Compensation for Heterozygous Disruption of the Kidney-Specific NaK2Cl Cotransporter Gene
J. Am. Soc. Nephrol., March 1, 2002; 13(3): 604 - 610.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. A. Bickel, J. G. Verbalis, M. A. Knepper, and C. A. Ecelbarger
Increased renal Na-K-ATPase, NCC, and beta -ENaC abundance in obese Zucker rats
Am J Physiol Renal Physiol, October 1, 2001; 281(4): F639 - F648.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
N. L. Nakhoul, K. S. Hering-Smith, S. M. Abdulnour-Nakhoul, and L. L. Hamm
Ammonium interaction with the epithelial sodium channel
Am J Physiol Renal Physiol, September 1, 2001; 281(3): F493 - F502.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
W. Wang, T.-H. Kwon, C. Li, A. Flyvbjerg, M. A. Knepper, J. Frokiar, and S. Nielsen
Altered expression of renal aquaporins and Na+ transporters in rats treated with L-type calcium blocker
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2001; 280(6): R1632 - R1641.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
T.-H. Kwon, C. Fulton, W. Wang, I. Kurtz, J. Frokiar, C. Aalkjar, and S. Nielsen
Chronic metabolic acidosis upregulates rat kidney Na-HCO3- cotransporters NBCn1 and NBC3 but not NBC1
Am J Physiol Renal Physiol, February 1, 2002; 282(2): F341 - F351.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online