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Am J Physiol Renal Physiol 279: F502-F508, 2000;
0363-6127/00 $5.00
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Vol. 279, Issue 3, F502-F508, September 2000

Vasopressin-dependent upregulation of aquaporin-2 gene expression in glucocorticoid-deficient rats

Takako Saito1, San-E Ishikawa1, Fumiko Ando1, Minori Higashiyama1, Shoichiro Nagasaka1, Sei Sasaki2, and Toshikazu Saito1

1 Division of Endocrinology and Metabolism, Department of Medicine, Jichi Medical School, Tochigi 329-0498; and 2 Second Department of Internal Medicine, Tokyo Medical and Dental University, Tokyo 113-0034, Japan

We determined alterations in renal aquaporin-2 (AQP2) gene expression in association with impaired water excretion in glucocorticoid-deficient rats. After adrenalectomy, Sprague-Dawley rats were administered aldosterone alone by osmotic pumps (glucocorticoid-deficient rats). As a control, both aldosterone and dexamethasone were administered. These animals were subjected to the studies on days 7-14. The expressions of AQP2 mRNA and protein in kidney of the glucocorticoid-deficient rats were increased by 1.6- and 1.4-fold compared with the control rats, respectively. An acute oral water load test verified the marked impairment in water excretion in the glucocorticoid-deficient rats. One hour after the water load, the expressions of AQP2 mRNA and protein were significantly reduced in the control rats, but they remained unchanged in the glucocorticoid-deficient rats. However, there was no alteration in [3H]arginine vasopressin (AVP) receptor binding and AVP V2 receptor mRNA expression in the glucocorticoid-deficient rats. A V2-receptor antagonist abolished the increased expressions of AQP2 mRNA and protein in the glucocorticoid-deficient rats. These results indicate that augmented expression of AQP2 participates in impaired water excretion, dependent on AVP, in glucocorticoid deficiency.

arginine vasopressin; water retention


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