| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
National Institute of Diabetes, and Digestive and Kidney Diseases, Bethesda, Maryland 20892
Experiments were performed in mice to investigate whether
cyclooxygenase-2 (COX-2) in epithelial cells near the tubulovascular contact point (macula densa and TAL cells) may regulate renin gene
expression in juxtaglomerular granular cells. Renin activity, afferent
arteriolar granularity, and renin mRNA were determined in wild-type
mice and in COX-2-knockout mice on control and low-NaCl diets. Renin
activity in microdissected glomeruli assessed as angiotensin I
formation in the presence of excess substrate and afferent arteriolar
granularity determined by direct visualization and immunostaining were
significantly reduced in COX-2 
/ compared with wild-type animals.
Similarly, renal cortical mRNA levels were lower in COX-2 / than in
wild-type mice. Maintaining mice on a low-salt diet for 14 days induced
an increase in renin mRNA, afferent arteriolar granularity, and renin
activity in wild-type mice. In contrast, renin mRNA and renin
granularity did not significantly increase in low-salt-treated COX-2
/ mice, whereas the increase in juxtaglomerular renin enzyme
activity was markedly attenuated, but not fully blocked. In additional
experiments we found that COX-2 mRNA was increased in angiotensin type
1A receptor-knockout mice compared with wild-type mice. We conclude
that COX-2 in the tubulovascular contact region is a critical
determinant of renin synthesis in granular cells under resting
conditions and that it participates in the stimulation of renin
expression caused by a low-NaCl intake.
renin activity; prostaglandins; macula densa; juxtaglomerular apparatus cyclooxygenase-2
This article has been cited by other articles:
|
|
 |
|
  C. Matzdorf, A. Kurtz, and K. Hocherl COX-2 activity determines the level of renin expression but is dispensable for acute upregulation of renin expression in rat kidneys Am J Physiol Renal Physiol, June 1, 2007; 292(6): F1782 - F1790. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Chen, S. M. Kim, M. Oppermann, R. Faulhaber-Walter, Y. Huang, D. Mizel, M. Chen, M. L. S. Lopez, L. S. Weinstein, R. A. Gomez, et al. Regulation of renin in mice with Cre recombinase-mediated deletion of G protein Gs{alpha} in juxtaglomerular cells Am J Physiol Renal Physiol, January 1, 2007; 292(1): F27 - F37. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Kim, L. Chen, D. Mizel, Y. G. Huang, J. P. Briggs, and J. Schnermann Low plasma renin and reduced renin secretory responses to acute stimuli in conscious COX-2-deficient mice Am J Physiol Renal Physiol, January 1, 2007; 292(1): F415 - F422. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. J. Mullins, M. A. Bailey, and J. J. Mullins Hypertension, Kidney, and Transgenics: A Fresh Perspective Physiol Rev, April 1, 2006; 86(2): 709 - 746. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Nusing, A. Treude, C. Weissenberger, B. Jensen, M. Bek, C. Wagner, S. Narumiya, and H. W. Seyberth Dominant Role of Prostaglandin E2 EP4 Receptor in Furosemide-Induced Salt-Losing Tubulopathy: A Model for Hyperprostaglandin E Syndrome/Antenatal Bartter Syndrome J. Am. Soc. Nephrol., August 1, 2005; 16(8): 2354 - 2362. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yang, Y. G. Huang, W. Ye, P. Hansen, J. B. Schnermann, and J. P. Briggs Influence of genetic background and gender on hypertension and renal failure in COX-2-deficient mice Am J Physiol Renal Physiol, June 1, 2005; 288(6): F1125 - F1132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Schweda, J. Klar, S. Narumiya, R. M. Nusing, and A. Kurtz Stimulation of renin release by prostaglandin E2 is mediated by EP2 and EP4 receptors in mouse kidneys Am J Physiol Renal Physiol, September 1, 2004; 287(3): F427 - F433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Paliege, D. Mizel, C. Medina, A. Pasumarthy, Y. G. Huang, S. Bachmann, J. P. Briggs, J. B. Schnermann, and T. Yang Inhibition of nNOS expression in the macula densa by COX-2-derived prostaglandin E2 Am J Physiol Renal Physiol, July 1, 2004; 287(1): F152 - F159. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Stubbe, B. L. Jensen, S. Bachmann, P. Morsing, and O. Skott Cyclooxygenase-2 contributes to elevated renin in the early postnatal period in rats Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2003; 284(5): R1179 - R1189. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. P. Connolly Cyclooxygenase-2 Inhibitors in Gynecologic Practice Clin. Med. Res., April 1, 2003; 1(2): 105 - 110. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Hartner, N. Cordasic, M. Goppelt-Struebe, R. Veelken, and K. F. Hilgers Role of macula densa cyclooxygenase-2 in renovascular hypertension Am J Physiol Renal Physiol, March 1, 2003; 284(3): F498 - F502. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Castrop, J. Klar, C. Wagner, K. Hocherl, and A. Kurtz General inhibition of renocortical cyclooxygenase-2 expression by the renin-angiotensin system Am J Physiol Renal Physiol, March 1, 2003; 284(3): F518 - F524. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ollerstam and A.E. G Persson Macula densa neuronal nitric oxide synthase Cardiovasc Res, November 1, 2002; 56(2): 189 - 196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hocherl, F. Dreher, H. Vitzthum, J. Kohler, and A. Kurtz Cyclosporine A Suppresses Cyclooxygenase-2 Expression in the Rat Kidney J. Am. Soc. Nephrol., October 1, 2002; 13(10): 2427 - 2436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-F. Cheng, S.-W. Wang, M.-Z. Zhang, J. A. McKanna, R. Breyer, and R. C. Harris Prostaglandins that increase renin production in response to ACE inhibition are not derived from cyclooxygenase-1 Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2002; 283(3): R638 - R646. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hocherl, M. C. Kammerl, K. Schumacher, D. Endemann, H. F. Grobecker, and A. Kurtz Role of prostanoids in regulation of the renin-angiotensin-aldosterone system by salt intake Am J Physiol Renal Physiol, August 1, 2002; 283(2): F294 - F301. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Schnermann Cyclooxygenase-2 and macula densa control of renin secretion Nephrol. Dial. Transplant., September 1, 2001; 16(9): 1735 - 1738. [Full Text] [PDF]
|
|
|
|
|
|
R. C. Harris and M. D. Breyer Physiological regulation of cyclooxygenase-2 in the kidney Am J Physiol Renal Physiol, July 1, 2001; 281(1): F1 - F11. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-F. Cheng, J.-L. Wang, M.-Z. Zhang, S.-W. Wang, James. A. McKanna, and R. C. Harris Genetic deletion of COX-2 prevents increased renin expression in response to ACE inhibition Am J Physiol Renal Physiol, March 1, 2001; 280(3): F449 - F456. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
