| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Renal Division, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30322
Angiotensin II receptors are present along the rat inner medullary collecting duct (IMCD), although their physiological role is unknown. Because urea is one of the major solutes transported across the terminal IMCD, we measured angiotensin II's effect on urea permeability. In the perfused rat terminal IMCD, angiotensin II had no effect on basal urea permeability but significantly increased vasopressin-stimulated urea permeability by 55%. Angiotensin II, both without and with vasopressin, also increased the amount of 32P incorporated into urea transporter (UT)-A1 in inner medullary tissue exposed to these hormones ex vivo. Because angiotensin II activates protein kinase C, we tested the effect of staurosporine (SSP). In the absence of angiotensin II, SSP had no effect on vasopressin-stimulated urea permeability in the perfused terminal IMCD. However, SSP completely and reversibly blocked the angiotensin II-mediated increase in vasopressin-stimulated urea permeability. SSP and chelerythrine reduced the angiotensin II-stimulated 32P incorporation into UT-A1 in inner medullary tissue exposed ex vivo. We conclude that angiotensin II increases vasopressin-stimulated facilitated urea permeability and 32P incorporation into the 97- and 117-kDa UT-A1 proteins via a protein kinase C-mediated signaling pathway. These data suggest that angiotensin II augments vasopressin-stimulated facilitated urea transport in the rat terminal IMCD and may play a physiological role in the urinary concentrating mechanism by augmenting the maximal response to vasopressin.
inner medullary collecting duct; protein kinase C; urine-concentrating mechanism; phosphorylation; hyperosmolality; urea transporter
This article has been cited by other articles:
|
|
 |
|
  T. Aoyagi, Y. Izumi, M. Hiroyama, T. Matsuzaki, Y. Yasuoka, A. Sanbe, H. Miyazaki, Y. Fujiwara, Y. Nakayama, Y. Kohda, et al. Vasopressin regulates the renin-angiotensin-aldosterone system via V1a receptors in macula densa cells Am J Physiol Renal Physiol, July 1, 2008; 295(1): F100 - F107. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Fenton and M. A. Knepper Mouse Models and the Urinary Concentrating Mechanism in the New Millennium Physiol Rev, October 1, 2007; 87(4): 1083 - 1112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-J. Lee, I.-K. Song, K.-J. Jang, J. Nielsen, J. Frokiaer, S. Nielsen, and T.-H. Kwon Increased AQP2 targeting in primary cultured IMCD cells in response to angiotensin II through AT1 receptor Am J Physiol Renal Physiol, January 1, 2007; 292(1): F340 - F350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Klein, B. P. Murrell, S. Tucker, Y.-H. Kim, and J. M. Sands Urea transporter UT-A1 and aquaporin-2 proteins decrease in response to angiotensin II or norepinephrine-induced acute hypertension Am J Physiol Renal Physiol, November 1, 2006; 291(5): F952 - F959. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Klein, O. Frohlich, M. A. Blount, C. F. Martin, T. D. Smith, and J. M. Sands Vasopressin Increases Plasma Membrane Accumulation of Urea Transporter UT-A1 in Rat Inner Medullary Collecting Ducts J. Am. Soc. Nephrol., October 1, 2006; 17(10): 2680 - 2686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Pech, J. D. Klein, S. D. Kozlowski, S. M. Wall, and J. M. Sands Vasopressin increases urea permeability in the initial IMCD from diabetic rats Am J Physiol Renal Physiol, September 1, 2005; 289(3): F531 - F535. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-H. Kwon, J. Nielsen, M. A. Knepper, J. Frokiaer, and S. Nielsen Angiotensin II AT1 receptor blockade decreases vasopressin-induced water reabsorption and AQP2 levels in NaCl-restricted rats Am J Physiol Renal Physiol, April 1, 2005; 288(4): F673 - F684. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Yao, D.-Y. Huang, I. L. Pfaff, X. Nie, M. Leitges, and V. Vallon Evidence for a role of protein kinase C-{alpha} in urine concentration Am J Physiol Renal Physiol, August 1, 2004; 287(2): F299 - F304. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zimpelmann, N. Li, and K. D. Burns Nitric oxide inhibits superoxide-stimulated urea permeability in the rat inner medullary collecting duct Am J Physiol Renal Physiol, December 1, 2003; 285(6): F1160 - F1167. [Abstract] [Full Text]
|
|
|
|
|
|
R. A. Fenton, C.-L. Chou, S. Ageloff, W. Brandt, J. B. Stokes, and M. A. Knepper Increased collecting duct urea transporter expression in Dahl salt-sensitive rats Am J Physiol Renal Physiol, July 1, 2003; 285(1): F143 - F151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Sands Molecular Approaches to Urea Transporters J. Am. Soc. Nephrol., November 1, 2002; 13(11): 2795 - 2806. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. E. Goodman TRANSPORT OF SMALL MOLECULES ACROSS CELL MEMBRANES: WATER CHANNELS AND UREA TRANSPORTERS Advan Physiol Educ, September 1, 2002; 26(3): 146 - 157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Klein, D. Le Quach, J. M. Cole, K. Disher, A. K. Mongiu, X. Wang, K. E. Bernstein, and J. M. Sands Impaired urine concentration and absence of tissue ACE: involvement of medullary transport proteins Am J Physiol Renal Physiol, September 1, 2002; 283(3): F517 - F524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Staahltoft, S. Nielsen, N. R. Janjua, S. Christensen, O. Skott, N. Marcussen, and T. E. N. Jonassen Losartan treatment normalizes renal sodium and water handling in rats with mild congestive heart failure Am J Physiol Renal Physiol, February 1, 2002; 282(2): F307 - F315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-Y. Wang, K. Beutler, J. Nielsen, S. Nielsen, M. A. Knepper, and S. Masilamani Decreased abundance of collecting duct urea transporters UT-A1 and UT-A3 with ECF volume expansion Am J Physiol Renal Physiol, April 1, 2002; 282(4): F577 - F584. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
