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1G-subunit of a voltage-dependent
Ca2+ channel is localized in rat distal nephron and
collecting duct
1 Department of Physiology and Pharmacology, University of Southern Denmark-Odense University, DK-5000 Odense; and 2 Department of Cell Biology, University of Aarhus, DK-8000 Aarhus, Denmark
The molecular type and localization of calcium channels along the
nephron are not well understood. In the present study, we assessed the
distribution of the recently identified 
1G-subunit encoding a voltage-dependent calcium channel with T-type
characteristics. Using a RNase protection assay, 1G-mRNA
levels in kidney regions were determined as inner medulla 
outer
medulla 
cortex. RT-PCR analysis of microdissected rat nephron
segments revealed 1G expression in the distal convoluted
tubule (DCT), in the connecting tubule and cortical collecting duct
(CT+CCD), and inner medullary collecting duct (IMCD). 1G
mRNA was expressed in the IMCD cell line mIMCD-3. Single- and
double-labeling immunohistochemistry and confocal laser microscopy on
semithin paraffin sections of rat kidneys by using an
anti-1G antibody demonstrated a distinct labeling at the
apical plasma membrane domains of DCT cells, CT principal cells, and
IMCD principal cells.
distal convoluted tubule; inner medullary collecting duct; voltage-operated calcium channel
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