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Am J Physiol Renal Physiol 280: F278-F282, 2001;
0363-6127/01 $5.00
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Vol. 280, Issue 2, F278-F282, February 2001

Na+-to-sugar stoichiometry of SGLT3

Ana Díez-Sampedro, Sepehr Eskandari, Ernest M. Wright, and Bruce A. Hirayama

Department of Physiology, University of California Los Angeles, School of Medicine, Los Angeles, California 90095-1751

Sodium-glucose cotransporters (SGLTs) mediate active transport of sugar across cell membranes coupled to Na+, by using the electrochemical gradient as a driving force. In the kidney, there is evidence for two kinds of cotransporters, a high-affinity, low-capacity system, and a low-affinity, high-capacity system, with differences in substrate specificity and kinetics. Three renal SGLT clones have been identified: SGLT1 corresponding to the high-affinity system, and SGLT2 and SGLT3 with properties reminiscent of the low-affinity system. We have determined the stoichiometry of pig SGLT3 (pSGLT3) by using a direct method, comparing the substrate-induced inward charge to 22Na or [14C]alpha -methyl-D-glucopyranoside uptake in the same oocyte. pSGLT3 stoichiometry is 2 Na+:1 sugar, the same as that for SGLT1, but different from SGLT2 (1:1). The Na+ Hill coefficient for SGLT3 is ~1.5, suggesting low cooperativity between Na+ binding sites. Thus SGLT3 has functional characteristics intermediate between SGLT1 and SGLT2, so, whereas SGLT3 stoichiometry is the same as that for SGLT1 (2:1), sugar affinity and specificity are similar to SGLT2.

sodium-glucose cotransporter; electrophysiology; ion-substrate coupling


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