AJP - Renal Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 280: F895-F903, 2001;
0363-6127/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (8)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Winkler, C. A.
Right arrow Articles by Schwartz, G. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Winkler, C. A.
Right arrow Articles by Schwartz, G. J.
Vol. 280, Issue 5, F895-F903, May 2001

Maturation of carbonic anhydrase IV expression in rabbit kidney

Cornelia A. Winkler, Ann M. Kittelberger, Richard H. Watkins, William M. Maniscalco, and George J. Schwartz

Department of Pediatrics, University of Rochester School of Medicine, Rochester, New York 14642

Carbonic anhydrase (CA) IV facilitates renal acidification by catalyzing the dehydration of luminal H2CO3. CA IV is expressed in proximal tubules, medullary collecting ducts, and A-intercalated cells of the mature rabbit kidney (Schwartz GJ, Kittelberger AM, Barnhart DA, and Vijayakumar S. Am J Physiol 278: F894-F904, 2000). In view of the maturation of HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> transport in the proximal tubule and collecting duct, the ontogeny of CA IV expression was examined. During the first 2 wk, CA IV mRNA was expressed in maturing cortex and medulla at ~20% of adult levels. The maturational increase was gradual in cortex over 3-5 wk of age but surged in the medulla, so that mRNA levels appeared higher than those in the adult medulla. In situ hybridization showed very little CA IV mRNA at 5 days, with increases in deep cortex and medullary collecting ducts by 21 days. Expression of CA IV protein in the cortex and medulla was minimal at 3 days of age but then apparent in the juxtamedullary region, A-intercalated cells and medullary collecting ducts by 18 days; there was little labeling of the proximal straight tubules of the medullary rays. Thus CA IV expression may be regulated to accommodate the maturational increase in HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> absorption in the proximal tubule. In the medullary collecting duct, there is a more robust maturation of CA IV mRNA and protein, commensurate with the high rate of HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> absorption in the neonatal segment.

kidney development; in situ hybridization; immunohistochemistry; Northern blot; medullary collecting duct; proximal tubule; intercalated cell


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
A. L. Schwaderer, S. Vijayakumar, Q. Al-Awqati, and G. J. Schwartz
Galectin-3 expression is induced in renal {beta}-intercalated cells during metabolic acidosis
Am J Physiol Renal Physiol, January 1, 2006; 290(1): F148 - F158.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. M. Purkerson and G. J. Schwartz
Expression of membrane-associated carbonic anhydrase isoforms IV, IX, XII, and XIV in the rabbit: induction of CA IV and IX during maturation
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2005; 288(5): R1256 - R1263.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
G. J. Schwartz, A. M. Kittelberger, R. H. Watkins, and M. A. O'Reilly
Carbonic anhydrase XII mRNA encodes a hydratase that is differentially expressed along the rabbit nephron
Am J Physiol Renal Physiol, February 1, 2003; 284(2): F399 - F410.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online