AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 280: F904-F912, 2001;
0363-6127/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tian, W.
Right arrow Articles by Cohen, D. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tian, W.
Right arrow Articles by Cohen, D. M.
Vol. 280, Issue 5, F904-F912, May 2001

Urea inhibits hypertonicity-inducible TonEBP expression and action

Wei Tian and David M. Cohen

Divisions of Nephrology and Molecular Medicine and Department of Cell and Developmental Biology, Oregon Health Sciences University and the Portland Veterans Affairs Medical Center, Portland, Oregon 97201

Tonicity-responsive genes are regulated by the TonE enhancer element and the tonicity-responsive enhancer binding protein (TonEBP) transcription factor with which it interacts. Urea, a permeant solute coexistent with hypertonic NaCl in the mammalian renal medulla, activates a characteristic set of signaling events that may serve to counteract the effects of NaCl in some contexts. Urea inhibited the ability of hypertonic stressors to increase expression of TonEBP mRNA and also inhibited tonicity-inducible TonE-dependent reporter gene activity. The permeant solute glycerol failed to reproduce these effects, as did cell activators including peptide mitogens and phorbol ester. The inhibitory effect of urea was evident as late as 2 h after the application of hypertonicity. Pharmacological inhibitors of known urea-inducible signaling pathways failed to abolish the inhibitory effect of urea. TonEBP action is incompletely understood, but evidence supports a role for proteasome function and p38 action in regulation; urea failed to inhibit proteasome function or p38 signaling in response to hypertonicity. Consistent with its effect on TonEBP expression and action, urea pretreatment inhibited the effect of hypertonicity on expression of the physiological effector gene, aldose reductase. Taken together, these data 1) define a molecular mechanism of urea-mediated inhibition of tonicity-dependent signaling, and 2) underscore a role for TonEBP abundance in regulating TonE-mediated gene transcription.

tonicity-responsive enhancer binding protein; stress; renal; kidney; cell culture; proteasome; mitogen-activated protein kinase


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
M. B. Burg, J. D. Ferraris, and N. I. Dmitrieva
Cellular Response to Hyperosmotic Stresses
Physiol Rev, October 1, 2007; 87(4): 1441 - 1474.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
W. Neuhofer and F.-X. Beck
Survival in Hostile Environments: Strategies of Renal Medullary Cells
Physiology, June 1, 2006; 21(3): 171 - 180.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
X. Zhou, J. D. Ferraris, Q. Cai, A. Agarwal, and M. B. Burg
Increased reactive oxygen species contribute to high NaCl-induced activation of the osmoregulatory transcription factor TonEBP/OREBP
Am J Physiol Renal Physiol, August 1, 2005; 289(2): F377 - F385.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
H. Xu, W. Tian, J. N. Lindsley, T. T. Oyama, J. M. Capasso, C. J. Rivard, H. T. Cohen, S. M. Bagnasco, S. Anderson, and D. M. Cohen
EphA2: expression in the renal medulla and regulation by hypertonicity and urea stress in vitro and in vivo
Am J Physiol Renal Physiol, April 1, 2005; 288(4): F855 - F866.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
F. Umenishi, S. Yoshihara, T. Narikiyo, and R. W. Schrier
Modulation of Hypertonicity-Induced Aquaporin-1 by Sodium Chloride, Urea, Betaine, and Heat Shock in Murine Renal Medullary Cells
J. Am. Soc. Nephrol., March 1, 2005; 16(3): 600 - 607.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Q. Cai, J. D. Ferraris, and M. B. Burg
Greater tolerance of renal medullary cells for a slow increase in osmolality is associated with enhanced expression of HSP70 and other osmoprotective genes
Am J Physiol Renal Physiol, January 1, 2004; 286(1): F58 - F67.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. A. Steinbrecher, J. A. Rudolph, G. Luo, and M. B. Cohen
Coordinate upregulation of guanylin and uroguanylin expression by hypertonicity in HT29-18-N2 cells
Am J Physiol Cell Physiol, December 1, 2002; 283(6): C1729 - C1737.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
W. Tian and D. M. Cohen
Urea stress is more akin to EGF exposure than to hypertonic stress in renal medullary cells
Am J Physiol Renal Physiol, September 1, 2002; 283(3): F388 - F398.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
H. Zhao, W. Tian, and D. M. Cohen
Rottlerin inhibits tonicity-dependent expression and action of TonEBP in a PKCdelta -independent fashion
Am J Physiol Renal Physiol, April 1, 2002; 282(4): F710 - F717.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online