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cotransporter NKCC1 to
Cl secretion in rat OMCD
Division of Renal Diseases and Hypertension, University of Texas Medical School at Houston, Houston, Texas 77030
In rat kidney the "secretory" isoform of the
Na+-K+-2Cl
cotransporter (NKCC1)
localizes to the basolateral membrane of the 
-intercalated cell. The
purpose of this study was to determine whether rat outer medullary
collecting duct (OMCD) secretes Cl and whether
transepithelial Cl transport occurs, in part, through
Cl uptake across the basolateral membrane mediated by
NKCC1 in series with Cl efflux across the apical
membrane. OMCD tubules from rats treated with deoxycorticosterone
pivalate were perfused in vitro in symmetrical HCO
secretion was observed in this segment, accompanied by
a lumen positive transepithelial potential. Bumetanide (100 µM), when added to the bath, reduced Cl secretion by 78%, although
the lumen positive transepithelial potential and fluid flux were
unchanged. Bumetanide-sensitive Cl secretion was
dependent on extracellular Na+ and either K+ or
NH
transport. In conclusion, OMCD tubules
from deoxycorticosterone pivalate-treated rats secrete Cl
into the luminal fluid through NKCC1-mediated Cl uptake
across the basolateral membrane in series with Cl efflux
across the apical membrane. The physiological role of NKCC1-mediated
Cl uptake remains to be determined. However, the role of
NKCC1 in the process of fluid secretion could not be demonstrated.
ammonium; type 1 bumetanide-sensitive sodium-potassium-2 chloride cotransporter; type 2 bumetanide-sensitive sodium-potassium-2 chloride cotransporter; fluid flux; chloride-bicarbonate exchange; outer medullary collecting duct
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