| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Institute of Experimental Clinical Research, University of Aarhus, DK-8200 Aarhus N; 2 Department of Clinical Physiology, Aarhus University Hospital-Skejby, DK-8200 Aarhus N; 3 Department of Cell Biology, Institute of Anatomy, University of Aarhus, DK-8000 Aarhus C, Denmark; 4 School of Biomedical Sciences, University of Leeds, Leeds LS2 9NQ, United Kingdom; and 5 Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892
Previously, we demonstrated that 24 h of bilateral ureteral obstruction (BUO) and short-term release of BUO was associated with a decrease in the expression of aquaporin-2 (AQP2), polyuria, and a reduced urinary concentrating capacity (10). The purposes of the present study were to examine whether BUO and the long-term release of BUO (BUO-R) for 3, 14, and 30 days were associated with changes in the expression of renal AQP1, AQP2, and AQP3 and whether such changes were associated with parallel changes in urinary output and urinary concentrating capacity. Rats (n = 4-7 in each group) were kept in metabolic cages for measurements of urinary output. Kidneys were removed to determine the expression levels of AQP1, AQP2, and AQP3 by semiquantitative immunoblotting. AQP2 was downregulated after 24 h of BUO (42 ± 3%). Downregulation of AQP2 persisted 3 (43 ± 14%; P < 0.01) and 15 days after BUO-R (48 ± 11%; P < 0.01) but was normalized 30 days after BUO-R. AQP3 showed a similar pattern. Moreover, AQP1 was downregulated in response to BUO (65 ± 7%) and remained downregulated 3 days after BUO-R (41 ± 5%), 14 days after BUO-R (57 ± 8%), and 30 days after BUO-R (59 ± 5%). BUO-R resulted in a significant polyuria that gradually decreased, although it remained significant at day 30. Urinary concentrating capacity remained significantly impaired when determined 3, 14, and 30 days after BUO-R in response to a 24-h period of thirst (1,712 ± 270 vs. 2,880 ± 91 mosmol/kgH2O at day 30, P < 0.05). In conclusion, the expression of AQP1, AQP2, and AQP3 were long-term downregulated after BUO-R, suggesting that dysregulation of aquaporins located at the proximal tubule, thin descending limb of the loop of Henle, and the collecting duct may contribute to the long-term polyuria and impairment of urinary concentrating capacity associated with obstructive nephropathy.
collecting duct; nephrogenic diabetes insipidus; obstructive nephropathy; polyuria; proximal tubule; aquaporin-1; aquaporin-2; aquaporin-3
This article has been cited by other articles:
|
|
 |
|
  M. Carlstrom, R. D. Brown, J. Sallstrom, E. Larsson, M. Zilmer, S. Zabihi, U. J. Eriksson, and A. E. G. Persson SOD1 deficiency causes salt sensitivity and aggravates hypertension in hydronephrosis Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2009; 297(1): R82 - R92. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Hasler, V. Leroy, P.-Y. Martin, and E. Feraille Aquaporin-2 abundance in the renal collecting duct: new insights from cultured cell models Am J Physiol Renal Physiol, July 1, 2009; 297(1): F10 - F18. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Norregaard, T. Bodker, B. L. Jensen, L. Stodkilde, S. Nielsen, and J. Frokiaer Increased renal adrenomedullin expression in rats with ureteral obstruction Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2009; 296(1): R185 - R192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Jensen, E. H. Bae, R. A. Fenton, R. Norregaard, S. Nielsen, S. W. Kim, and J. Frokiaer Angiotensin II regulates V2 receptor and pAQP2 during ureteral obstruction Am J Physiol Renal Physiol, January 1, 2009; 296(1): F127 - F134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. H. M. Shimizu, A. Danilovic, L. Andrade, R. A. Volpini, A. B. Liborio, T. R.C. Sanches, and A. C. Seguro N-acetylcysteine protects against renal injury following bilateral ureteral obstruction Nephrol. Dial. Transplant., October 1, 2008; 23(10): 3067 - 3073. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Wang, C. Li, S. W. Kim, T. Ring, J. Wen, J. C. Djurhuus, W. Wang, S. Nielsen, and J. Frokiaer Ureter obstruction alters expression of renal acid-base transport proteins in rat kidney Am J Physiol Renal Physiol, August 1, 2008; 295(2): F497 - F506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Carlstrom, R. D. Brown, J. Edlund, J. Sallstrom, E. Larsson, T. Teerlink, F. Palm, N. Wahlin, and A. E. G. Persson Role of nitric oxide deficiency in the development of hypertension in hydronephrotic animals Am J Physiol Renal Physiol, February 1, 2008; 294(2): F362 - F370. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, W. Wang, R. Norregaard, M. A. Knepper, S. Nielsen, and J. Frokiaer Altered expression of epithelial sodium channel in rats with bilateral or unilateral ureteral obstruction Am J Physiol Renal Physiol, July 1, 2007; 293(1): F333 - F341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Norregaard, B. L. Jensen, S. O. Topcu, M. Diget, H. Schweer, M. A. Knepper, S. Nielsen, and J. Frokiaer COX-2 activity transiently contributes to increased water and NaCl excretion in the polyuric phase after release of ureteral obstruction Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1322 - F1333. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Eskild-Jensen, K. Thomsen, C. Rungo, L. S. Ferreira, L. F. Paulsen, Y. F. Rawashdeh, J. R. Nyengaard, S. Nielsen, J. C. Djurhuus, and J. Frokiaer Glomerular and tubular function during AT1 receptor blockade in pigs with neonatal induced partial ureteropelvic obstruction Am J Physiol Renal Physiol, March 1, 2007; 292(3): F921 - F929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. O. Topcu, M. Pedersen, R. Norregaard, G. Wang, M. Knepper, J. C. Djurhuus, S. Nielsen, T. M. Jorgensen, and J. Frokiaer Candesartan prevents long-term impairment of renal function in response to neonatal partial unilateral ureteral obstruction Am J Physiol Renal Physiol, February 1, 2007; 292(2): F736 - F748. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Jensen, C. Li, H. A. Praetorius, R. Norregaard, S. Frische, M. A. Knepper, S. Nielsen, and J. Frokiaer Angiotensin II mediates downregulation of aquaporin water channels and key renal sodium transporters in response to urinary tract obstruction Am J Physiol Renal Physiol, November 1, 2006; 291(5): F1021 - F1032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Robben, N. V. A. M. Knoers, and P. M. T. Deen Cell biological aspects of the vasopressin type-2 receptor and aquaporin 2 water channel in nephrogenic diabetes insipidus. Am J Physiol Renal Physiol, August 1, 2006; 291(2): F257 - F270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Yang, D. Zhao, L. Qian, and A. S. Verkman Mouse model of inducible nephrogenic diabetes insipidus produced by floxed aquaporin-2 gene deletion Am J Physiol Renal Physiol, August 1, 2006; 291(2): F465 - F472. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wang, F. Amidi, M. Beall, L. Gui, and M. G. Ross Aquaporin 3 Expression in Human Fetal Membranes and its Up-regulation by Cyclic Adenosine Monophosphate in Amnion Epithelial Cell Culture Reproductive Sciences, April 1, 2006; 13(3): 181 - 185. [Abstract] [PDF]
|
|
|
|
|
|
C. Li, Y. Shi, W. Wang, C. Sardeli, T.-H. Kwon, K. Thomsen, T. Jonassen, J. C. Djurhuus, M. A. Knepper, S. Nielsen, et al. {alpha}-MSH prevents impairment in renal function and dysregulation of AQPs and Na-K-ATPase in rats with bilateral ureteral obstruction Am J Physiol Renal Physiol, February 1, 2006; 290(2): F384 - F396. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Cochrane, M. M. Kett, C. S. Samuel, N. V. Campanale, W. P. Anderson, D. A. Hume, M. H. Little, J. F. Bertram, and S. D. Ricardo Renal Structural and Functional Repair in a Mouse Model of Reversal of Ureteral Obstruction J. Am. Soc. Nephrol., December 1, 2005; 16(12): 3623 - 3630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Norregaard, B. L. Jensen, C. Li, W. Wang, M. A. Knepper, S. Nielsen, and J. Frokiaer COX-2 inhibition prevents downregulation of key renal water and sodium transport proteins in response to bilateral ureteral obstruction Am J Physiol Renal Physiol, August 1, 2005; 289(2): F322 - F333. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Murer, F. Addabbo, M. Carmosino, G. Procino, G. Tamma, G. Montini, W. Rigamonti, P. Zucchetta, M. Della Vella, A. Venturini, et al. Selective Decrease in Urinary Aquaporin 2 and Increase in Prostaglandin E2 Excretion Is Associated with Postobstructive Polyuria in Human Congenital Hydronephrosis J. Am. Soc. Nephrol., October 1, 2004; 15(10): 2705 - 2712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shi, M. Pedersen, C. Li, J. G. Wen, K. Thomsen, H. Stodkilde-Jorgensen, T. M. Jorgensen, M. A. Knepper, S. Nielsen, J. C. Djurhuus, et al. Early release of neonatal ureteral obstruction preserves renal function Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1087 - F1099. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, J. D. Klein, W. Wang, M. A. Knepper, S. Nielsen, J. M. Sands, and J. Frokiaer Altered expression of urea transporters in response to ureteral obstruction Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1154 - F1162. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, W. Wang, T.-H. Kwon, M. A. Knepper, S. Nielsen, and J. Frokiaer Altered expression of major renal Na transporters in rats with bilateral ureteral obstruction and release of obstruction Am J Physiol Renal Physiol, November 1, 2003; 285(5): F889 - F901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, W. Wang, M. A. Knepper, S. Nielsen, and J. Frokiar Downregulation of renal aquaporins in response to unilateral ureteral obstruction Am J Physiol Renal Physiol, May 1, 2003; 284(5): F1066 - F1079. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, W. Wang, T.-H. Kwon, M. A. Knepper, S. Nielsen, and J. Frokiar Altered expression of major renal Na transporters in rats with unilateral ureteral obstruction Am J Physiol Renal Physiol, January 1, 2003; 284(1): F155 - F166. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nielsen, J. Frokiar, D. Marples, T.-H. Kwon, P. Agre, and M. A. Knepper Aquaporins in the Kidney: From Molecules to Medicine Physiol Rev, January 1, 2002; 82(1): 205 - 244. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
