AJP - Renal Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 281: F81-F90, 2001;
0363-6127/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (19)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hannken, T.
Right arrow Articles by Wolf, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hannken, T.
Right arrow Articles by Wolf, G.
Vol. 281, Issue 1, F81-F90, July 2001

Atrial natriuretic peptide attenuates ANG II-induced hypertrophy of renal tubular cells

Tete Hannken, Regine Schroeder, Rolf A. K. Stahl, and Gunter Wolf

Department of Medicine, Division of Nephrology and Osteology, University of Hamburg, D-20246 Hamburg, Germany

ANG II arrests LLC-PK1 cells in the G1 phase of the cell cycle and induces hypertrophy, an effect mediated by induction of p27Kip1. We studied whether atrial natriuretic peptide (ANP) may modulate ANG II-induced hypertrophy and p27Kip1 expression in tubular LLC-PK1 cells. ANP, through its fragments 3---28 and 4---27, prevented ANG II-induced cell cycle arrest. ANP inhibited >80% of ANG II-induced p27Kip1 protein expression (Western blots). ANP stimulated expression of MKP-1, a phosphatase involved in dephosphorylation of p44/42 mitogen-activated protein (MAP) kinase, up to 12 h. ANP prevented the ANG II-mediated phosphorylation peak of MAP kinase after 12 h of stimulation. 8-Bromo-cGMP mimicked all the effects of ANP. Transfection with MKP-1 antisense, but not sense, oligonucleotides abolished the modifying role of ANP on ANG II-mediated cell cycle arrest.. The effect of ANP on ANG II-mediated hypertrophy of LLC-PK1 cells is regulated on the level of MAP kinase phosphorylation, a key step in the induction of p27Kip1. Although ANP and ANG II both stimulate generation of reactive oxygen species, ANP additionally induces expression of MKP-1, leading to interference with ANG II-mediated MAP kinase phosphorylation.

tubular hypertrophy; cell cycle arrest; signal transduction; LLC-PK1 cells; mitogen-activated protein kinases


This article has been cited by other articles:


Home page
 Nephrol Dial TransplantHome page
E. Gava, A. Samad-Zadeh, J. Zimpelmann, N. Bahramifarid, G. T. Kitten, R. A. Santos, R. M. Touyz, and K. D. Burns
Angiotensin-(1-7) activates a tyrosine phosphatase and inhibits glucose-induced signalling in proximal tubular cells
Nephrol. Dial. Transplant., June 1, 2009; 24(6): 1766 - 1773.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
R. Furst, C. Brueckl, W. M. Kuebler, S. Zahler, F. Krotz, A. Gorlach, A. M. Vollmar, and A. K. Kiemer
Atrial Natriuretic Peptide Induces Mitogen-Activated Protein Kinase Phosphatase-1 in Human Endothelial Cells via Rac1 and NAD(P)H Oxidase/Nox2-Activation
Circ. Res., January 7, 2005; 96(1): 43 - 53.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. M. Devlin, N. Solban, S. Tremblay, J. Gutkowska, W. Schurch, S. N. Orlov, R. Lewanczuk, P. Hamet, and J. Tremblay
HCaRG is a novel regulator of renal epithelial cell growth and differentiation causing G2M arrest
Am J Physiol Renal Physiol, April 1, 2003; 284(4): F753 - F762.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
A. K. Kiemer, N. C. Weber, R. Furst, N. Bildner, S. Kulhanek-Heinze, and A. M. Vollmar
Inhibition of p38 MAPK Activation via Induction of MKP-1: Atrial Natriuretic Peptide Reduces TNF-{alpha}-Induced Actin Polymerization and Endothelial Permeability
Circ. Res., May 3, 2002; 90(8): 874 - 881.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
A. K. Kiemer, N. C. Weber, R. Furst, N. Bildner, S. Kulhanek-Heinze, and A. M. Vollmar
Inhibition of p38 MAPK Activation via Induction of MKP-1: Atrial Natriuretic Peptide Reduces TNF-{alpha}-Induced Actin Polymerization and Endothelial Permeability
Circ. Res., May 3, 2002; 90(8): 874 - 881.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online