AJP - Renal Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 281: F206-F212, 2001;
0363-6127/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (36)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wei, Y.
Right arrow Articles by Wang, W. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wei, Y.
Right arrow Articles by Wang, W. H.
Vol. 281, Issue 2, F206-F212, August 2001

Effect of dietary K intake on apical small-conductance K channel in CCD: role of protein tyrosine kinase

Yuan Wei, Peter Bloom, Daohong Lin, Ruimin Gu, and Wen Hui Wang

Department of Pharmacology, New York Medical College, Valhalla, New York 10595

We have used Western blot to examine the expression of cSrc protein tyrosine kinase (PTK) and protein tyrosine phosphatase (PTP)-1D in the renal cortex, and the patch-clamp technique to determine the role of PTK in mediating the effect of dietary K intake on the small-conductance K (SK) channel in the cortical collecting duct (CCD). When rats were on a K-deficient (KD) diet for 1, 3, 5, and 7 days, the expression of cSrc increased by 40, 90, 140, and 135%, respectively. In contrast, the expression of cSrc in the renal cortex from rats on a high-K (HK) diet for 1, 2, and 3 days decreased by 40, 60, and 75%, respectively. However, the protein level of PTP-1D was not significantly changed by dietary K intake. The addition of 1 µM herbimycin A increased NPo, a product of channel number (N) and open probability (Po) in the CCD from rats on a normal diet or on a KD diet. The increase in NPo was 0.30 (normal), 0.45 (1-day KD), 0.65 (3-day KD), 1.55 (5-day KD), and 1.85 (7-day KD), respectively. Treatment of the CCD with herbimycin A from rats on a KD diet increased NPo per patch from the control value (0.7) to 1.4 (1-day KD), 1.6 (3-day KD), 2.6 (5-day KD), and 3.5 (7-day KD), respectively. In contrast, HK intake for as short as 1 day abolished the effect of herbimycin A. Furthermore, the expression of ROMK channels in the renal cortex was the same between rats on a KD diet or on a HK diet. Moreover, treatment with herbimycin A did not further increase NPo in the CCDs from rats on a HK diet. We conclude that dietary K intake plays a key role in regulating the activity of the SK channels and that PTK is involved in mediating the effect of the K intake on channel activity in the CCD.

hyperkalemia; hypokalemia; protein tyrosine phosphatase 1D; renal potassium secretion; cortical collecting duct


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
D.-H. Lin, P. Yue, C.-Y. Pan, P. Sun, X. Zhang, Z. Han, M. Roos, M. Caplan, G. Giebisch, and W.-H. Wang
POSH Stimulates the Ubiquitination and the Clathrin-independent Endocytosis of ROMK1 Channels
J. Biol. Chem., October 23, 2009; 284(43): 29614 - 29624.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
P. A. Welling and K. Ho
A comprehensive guide to the ROMK potassium channel: form and function in health and disease
Am J Physiol Renal Physiol, October 1, 2009; 297(4): F849 - F863.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
P. Yue, D.-H. Lin, C.-Y. Pan, Q. Leng, G. Giebisch, R. P. Lifton, and W.-H. Wang
Src family protein tyrosine kinase (PTK) modulates the effect of SGK1 and WNK4 on ROMK channels
PNAS, September 1, 2009; 106(35): 15061 - 15066.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
G. Frindt, A. Shah, J. Edvinsson, and L. G. Palmer
Dietary K regulates ROMK channels in connecting tubule and cortical collecting duct of rat kidney
Am J Physiol Renal Physiol, February 1, 2009; 296(2): F347 - F354.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
R. C. Williamson, A. C. N. Brown, W. J. Mawby, and A. M. Toye
Human kidney anion exchanger 1 localisation in MDCK cells is controlled by the phosphorylation status of two critical tyrosines
J. Cell Sci., October 15, 2008; 121(20): 3422 - 3432.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
E. Babilonia, D. Lin, Y. Zhang, Y. Wei, P. Yue, and W.-H. Wang
Role of gp91phox-Containing NADPH Oxidase in Mediating the Effect of K Restriction on ROMK Channels and Renal K Excretion
J. Am. Soc. Nephrol., July 1, 2007; 18(7): 2037 - 2045.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
U. S. Jeon, K.-H. Han, S.-H. Park, S. D. Lee, M. R. Sheen, J.-Y. Jung, W. Y. Kim, J. M. Sands, J. Kim, and H. M. Kwon
Downregulation of renal TonEBP in hypokalemic rats
Am J Physiol Renal Physiol, July 1, 2007; 293(1): F408 - F415.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Y. Wei, Z. Wang, E. Babilonia, H. Sterling, P. Sun, and W. Wang
Effect of hydrogen peroxide on ROMK channels in the cortical collecting duct
Am J Physiol Renal Physiol, April 1, 2007; 292(4): F1151 - F1156.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
E. Babilonia, D. Li, Z. Wang, P. Sun, D.-H. Lin, Y. Jin, and W.-H. Wang
Mitogen-Activated Protein Kinases Inhibit the ROMK (Kir 1.1)-Like Small Conductance K Channels in the Cortical Collecting Duct
J. Am. Soc. Nephrol., October 1, 2006; 17(10): 2687 - 2696.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
P. Chen, J. P. Guzman, P. K. K. Leong, L. E. Yang, A. Perianayagam, E. Babilonia, J. S. Ho, J. H. Youn, W. H. Wang, and A. A. McDonough
Modest dietary K+ restriction provokes insulin resistance of cellular K+ uptake and phosphorylation of renal outer medulla K+ channel without fall in plasma K+ concentration
Am J Physiol Cell Physiol, May 1, 2006; 290(5): C1355 - C1363.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. Li, Y. Wei, E. Babilonia, Z. Wang, and W.-H. Wang
Inhibition of phosphatidylinositol 3-kinase stimulates activity of the small-conductance K channel in the CCD
Am J Physiol Renal Physiol, April 1, 2006; 290(4): F806 - F812.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
W.-H. Wang
Regulation of ROMK (Kir1.1) channels: new mechanisms and aspects
Am J Physiol Renal Physiol, January 1, 2006; 290(1): F14 - F19.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Y. Wei, E. Babilonia, H. Sterling, Y. Jin, and W.-H. Wang
Mineralocorticoids decrease the activity of the apical small-conductance K channel in the cortical collecting duct
Am J Physiol Renal Physiol, November 1, 2005; 289(5): F1065 - F1071.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. A. Gray, G. Frindt, and L. G. Palmer
Quantification of K+ secretion through apical low-conductance K channels in the CCD
Am J Physiol Renal Physiol, July 1, 2005; 289(1): F117 - F126.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
D.-H. Lin, H. Sterling, and W.-H. Wang
The Protein Tyrosine Kinase-Dependent Pathway Mediates the Effect of K Intake on Renal K Secretion
Physiology, April 1, 2005; 20(2): 140 - 146.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
S. C. Hebert, G. Desir, G. Giebisch, and W. Wang
Molecular Diversity and Regulation of Renal Potassium Channels
Physiol Rev, January 1, 2005; 85(1): 319 - 371.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
P. Meneton, J. Loffing, and D. G. Warnock
Sodium and potassium handling by the aldosterone-sensitive distal nephron: the pivotal role of the distal and connecting tubule
Am J Physiol Renal Physiol, October 1, 2004; 287(4): F593 - F601.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Y. Wei, Y.-J. Chen, D. Li, R. Gu, and W.-H. Wang
Dual effect of insulin-like growth factor on the apical 70-pS K channel in the thick ascending limb of rat kidney
Am J Physiol Cell Physiol, June 1, 2004; 286(6): C1258 - C1263.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
H. Sterling, D.-H. Lin, Y.-J. Chen, Y. Wei, Z.-J. Wang, J. Lai, and W.-H. Wang
PKC expression is regulated by dietary K intake and mediates internalization of SK channels in the CCD
Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1072 - F1078.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D.-H. Lin, H. Sterling, B. Yang, S. C. Hebert, G. Giebisch, and W.-H. Wang
Protein tyrosine kinase is expressed and regulates ROMK1 location in the cortical collecting duct
Am J Physiol Renal Physiol, May 1, 2004; 286(5): F881 - F892.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
H. Sterling, D.-H. Lin, Y. Wei, and W.-H. Wang
Tetanus toxin abolishes exocytosis of ROMK1 induced by inhibition of protein tyrosine kinase
Am J Physiol Renal Physiol, March 1, 2003; 284(3): F510 - F517.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D.-H. Lin, H. Sterling, K. M. Lerea, P. Welling, L. Jin, G. Giebisch, and W.-H. Wang
K depletion increases protein tyrosine kinase-mediated phosphorylation of ROMK
Am J Physiol Renal Physiol, October 1, 2002; 283(4): F671 - F677.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Y. Wei and W.-H. Wang
Role of the cytoskeleton in mediating effect of vasopressin and herbimycin A on secretory K channels in CCD
Am J Physiol Renal Physiol, April 1, 2002; 282(4): F680 - F686.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Sterling, D.-H. Lin, R.-M. Gu, K. Dong, S. C. Hebert, and W.-H. Wang
Inhibition of Protein-tyrosine Phosphatase Stimulates the Dynamin-dependent Endocytosis of ROMK1
J. Biol. Chem., February 1, 2002; 277(6): 4317 - 4323.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
R.-M. Gu, Y. Wei, H.-L. Jiang, D.-H. Lin, H. Sterling, P. Bloom, M. Balazy, and W.-H. Wang
K Depletion Enhances the Extracellular Ca2+-Induced Inhibition of the Apical K Channels in the Mtal of Rat Kidney
J. Gen. Physiol., January 1, 2002; 119(1): 33 - 44.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online