AJP - Renal Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 281: F288-F292, 2001;
0363-6127/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (21)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, T.
Right arrow Articles by Aronson, P. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, T.
Right arrow Articles by Aronson, P. S.
Vol. 281, Issue 2, F288-F292, August 2001

Essential role of NHE3 in facilitating formate-dependent NaCl absorption in the proximal tubule

Tong Wang1, Chao-Ling Yang2, Thecla Abbiati2, Gary E. Shull3, Gerhard Giebisch1, and Peter S. Aronson1,2

Departments of 1 Cellular and Molecular Physiology, and 2 Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520-8029; and 3 Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0524

The absorption of NaCl in the proximal tubule is markedly stimulated by formate. This stimulation of NaCl transport is consistent with a cell model involving Cl--formate exchange in parallel with pH-coupled formate recycling due to nonionic diffusion of formic acid or H+-formate cotransport. The formate recycling process requires H+ secretion. Although Na+-H+ exchanger isoform NHE3 accounts for the largest component of H+ secretion in the proximal tubule, 40-50% of the rates of HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> absorption or cellular H+ extrusion persist in NHE3 null mice. The purpose of the present investigation is to use NHE3 null mice to directly test the role of apical membrane NHE3 in mediating NaCl absorption stimulated by formate. We demonstrate that formate stimulates NaCl absorption in the mouse proximal tubule microperfused in vivo, but the component of NaCl absorption stimulated by formate is absent in NHE3 null mice. In contrast, stimulation of NaCl absorption by oxalate is preserved in NHE3 null mice, indicating that oxalate-stimulated NaCl absorption is independent of Na+-H+ exchange. The virtually complete dependence of formate-induced NaCl absorption on NHE3 activity raises the possibility that NHE3 and the formate transporters are functionally coupled in the brush border membrane.

oxalate; anion exchange; Na+-H+ exchanger; microperfusion


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
E. M. Bradford, M. A. Sartor, L. R. Gawenis, L. L. Clarke, and G. E. Shull
Reduced NHE3-mediated Na+ absorption increases survival and decreases the incidence of intestinal obstructions in cystic fibrosis mice
Am J Physiol Gastrointest Liver Physiol, April 1, 2009; 296(4): G886 - G898.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H. A. Hassan, S. Mentone, L. P. Karniski, V. M. Rajendran, and P. S. Aronson
Regulation of anion exchanger Slc26a6 by protein kinase C
Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1485 - C1492.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. G. Fuster, I. A. Bobulescu, J. Zhang, J. Wade, and O. W. Moe
Characterization of the regulation of renal Na+/H+ exchanger NHE3 by insulin
Am J Physiol Renal Physiol, February 1, 2007; 292(2): F577 - F585.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. Deng, C. M. Miracle, M. Lortie, J. Satriano, F. B. Gabbai, K. A. Munger, S. C. Thomson, and R. C. Blantz
Kidney oxygen consumption, carbonic anhydrase, and proton secretion
Am J Physiol Renal Physiol, May 1, 2006; 290(5): F1009 - F1015.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
P. L. Dudas, S. Mentone, C. F. Greineder, D. Biemesderfer, and P. S. Aronson
Immunolocalization of anion transporter Slc26a7 in mouse kidney
Am J Physiol Renal Physiol, April 1, 2006; 290(4): F937 - F945.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Z. Wang, T. Wang, S. Petrovic, B. Tuo, B. Riederer, S. Barone, J. N. Lorenz, U. Seidler, P. S. Aronson, and M. Soleimani
Renal and intestinal transport defects in Slc26a6-null mice
Am J Physiol Cell Physiol, April 1, 2005; 288(4): C957 - C965.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. Goyal, S. Mentone, and P. S. Aronson
Immunolocalization of NHE8 in rat kidney
Am J Physiol Renal Physiol, March 1, 2005; 288(3): F530 - F538.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. Petrovic, S. Barone, A. M. Weinstein, and M. Soleimani
Activation of the apical Na+/H+ exchanger NHE3 by formate: a basis of enhanced fluid and electrolyte reabsorption by formate in the kidney
Am J Physiol Renal Physiol, August 1, 2004; 287(2): F336 - F346.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
K. W. Beyenbach
Kidneys sans glomeruli
Am J Physiol Renal Physiol, May 1, 2004; 286(5): F811 - F827.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. M. Weinstein
Mathematical models of renal fluid and electrolyte transport: acknowledging our uncertainty
Am J Physiol Renal Physiol, May 1, 2003; 284(5): F871 - F884.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. P. Karniski, T. Wang, L. A. Everett, E. D. Green, G. Giebisch, and P. S. Aronson
Formate-stimulated NaCl absorption in the proximal tubule is independent of the pendrin protein
Am J Physiol Renal Physiol, November 1, 2002; 283(5): F952 - F956.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Q. Xie, R. Welch, A. Mercado, M. F. Romero, and D. B. Mount
Molecular characterization of the murine Slc26a6 anion exchanger: functional comparison with Slc26a1
Am J Physiol Renal Physiol, October 1, 2002; 283(4): F826 - F838.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z. Jiang, I. I. Grichtchenko, W. F. Boron, and P. S. Aronson
Specificity of Anion Exchange Mediated by Mouse Slc26a6
J. Biol. Chem., September 6, 2002; 277(37): 33963 - 33967.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Klisic, M. C. Hu, V. Nief, L. Reyes, D. Fuster, O. W. Moe, and P. M. Ambuhl
Insulin activates Na+/H+ exchanger 3: biphasic response and glucocorticoid dependence
Am J Physiol Renal Physiol, September 1, 2002; 283(3): F532 - F539.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online