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2c-subunit in rabbit kidney
Nephrology and Hypertension, Department of Veterans Affairs Medical Center, Gainesville, Florida 32608-1197
The rabbit kidney
possesses mRNA for the H-K-ATPase 
1-subunit
(HK1) and two splice variants of the H-K-ATPase
2-subunit (HK2). The purpose of this
study was to determine the specific distribution of one of these, the
H-K-ATPase 2c-subunit isoform (HK2c), in
rabbit kidney by immunohistochemistry. Chicken polyclonal antibodies
against a peptide based on the NH2 terminus of
HK2c were used to detect HK2c
immunoreactivity in tissue sections. Immunohistochemical localization
of HK2c revealed intense apical immunoreactivity in a
subpopulation of cells in the connecting segment, cortical collecting
duct, and outer medullary collecting duct in both the outer and inner
stripe. An additional population of cells exhibited a thin apical band
of immunolabel. Immunohistochemical colocalization of
HK2c with carbonic anhydrase II, the
Cl
/HCO
1 indicated that both type A and type B intercalated
cells possessed intense apical HK2c immunoreactivity,
whereas principal cells and connecting segment cells had only a thin
apical band of HK2c. Labeled cells were evident through
the middle third of the inner medullary collecting duct in the majority
of animals. Immunolabel was also present in papillary surface
epithelial cells, cells in the cortical thick ascending limb of
Henle's loop (cTAL), and the macula densa. Thus in the rabbit kidney,
apical HK2c is present and may contribute to acid
secretion or potassium uptake throughout the connecting segment and
collecting duct in both type A and type B intercalated cells, principal
cells, and connecting segment cells, as well as in cells in papillary
surface epithelium, cTAL, and macula densa.
hypokalemia; anatomy; renal function
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