AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 281: F687-F692, 2001;
0363-6127/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (5)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dijkink, L.
Right arrow Articles by Bindels, R. J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dijkink, L.
Right arrow Articles by Bindels, R. J. M.
Vol. 281, Issue 4, F687-F692, October 2001

Modulation of aldosterone-induced stimulation of ENaC synthesis by changing the rate of apical Na+ entry

Lisette Dijkink, Anita Hartog, Carel H. Van Os, and René J. M. Bindels

Department of Cell Physiology, University Medical Centre Nijmegen, 6500 HB Nijmegen, The Netherlands

Primary cultures of immunodissected rabbit connecting tubule and cortical collecting duct cells were used to investigate the effect of apical Na+ entry rate on aldosterone-induced transepithelial Na+ transport, which was measured as benzamil-sensitive short-circuit current (Isc). Stimulation of the apical Na+ entry, by long-term short-circuiting of the monolayers, suppressed the aldosterone-stimulated benzamil-sensitive Isc from 320 ± 49 to 117 ± 14%, whereas in the presence of benzamil this inhibitory effect was not observed (335 ± 74%). Immunoprecipitation of [35S]methionine-labeled beta -rabbit epithelial Na+ channel (rbENaC) revealed that the effects of modulation of apical Na+ entry on transepithelial Na+ transport are exactly mirrored by beta -rbENaC protein levels, because short-circuiting the monolayers decreased aldosterone-induced beta -rbENaC protein synthesis from 310 ± 51 to 56 ± 17%. Exposure to benzamil doubled the beta -rbENaC protein level to 281 ± 68% in control cells but had no significant effect on aldosterone-stimulated beta -rbENaC levels (282 ± 68%). In conclusion, stimulation of apical Na+ entry suppresses the aldosterone-induced increase in transepithelial Na+ transport. This negative-feedback inhibition is reflected in a decrease in beta -rbENaC synthesis or in an increase in beta -rbENaC degradation.

rabbit kidney; cortical collecting duct; connecting tubule; epithelial sodium channel; benzamil


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
S. W. Kim, W. Wang, J. Nielsen, J. Praetorius, T.-H. Kwon, M. A. Knepper, J. Frokiaer, and S. Nielsen
Increased expression and apical targeting of renal ENaC subunits in puromycin aminonucleoside-induced nephrotic syndrome in rats
Am J Physiol Renal Physiol, May 1, 2004; 286(5): F922 - F935.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Nielsen, T.-H. Kwon, J. Praetorius, Y.-H. Kim, J. Frokiaer, M. A. Knepper, and S. Nielsen
Segment-specific ENaC downregulation in kidney of rats with lithium-induced NDI
Am J Physiol Renal Physiol, December 1, 2003; 285(6): F1198 - F1209.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Lebowitz, B. An, R. S. Edinger, M. L. Zeidel, and J. P. Johnson
Effect of altered Na+ entry on expression of apical and basolateral transport proteins in A6 epithelia
Am J Physiol Renal Physiol, September 1, 2003; 285(3): F524 - F531.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
S. Kellenberger and L. Schild
Epithelial Sodium Channel/Degenerin Family of Ion Channels: A Variety of Functions for a Shared Structure
Physiol Rev, July 1, 2002; 82(3): 735 - 767.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online