AJP - Renal Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 281: F958-F965, 2001;
0363-6127/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (22)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jo, I.
Right arrow Articles by Harris, H. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jo, I.
Right arrow Articles by Harris, H. W.
Vol. 281, Issue 5, F958-F965, November 2001

AQP2 is a substrate for endogenous PP2B activity within an inner medullary AKAP-signaling complex

Inho Jo1,2,*, Donald T. Ward1,3,*, Michelle A. Baum1, John D. Scott4, Vincent M. Coghlan5, Timothy G. Hammond6, and H. William Harris1,7

1 Division of Nephrology, Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115; 2 Department of Biomedical Sciences, National Institute of Health, Seoul, Korea; 3 School of Biological Sciences, University of Manchester, Manchester M13 9PT, United Kingdom; 4 Vollum Institute, Howard Hughes Medical Institute, and 5 Neurological Sciences Institute, Oregon Health Sciences University, Portland, Oregon 97201; 6 Tulane University Medical Center and Veterans Affairs Medical Center, New Orleans, Louisiana 70112; and 7 Maine Medical Center Research Institute, Portland, Maine 04102

We have demonstrated that inner medullary collecting duct (IMCD) heavy endosomes purified from rat kidney IMCD contain the type II protein kinase A (PKA) regulatory subunit (RII), protein phosphatase (PP)2B, PKCzeta , and an RII-binding protein (relative molecular mass ~90 kDa) representing a putative A kinase anchoring protein (AKAP). Affinity chromatography of detergent-solubilized endosomes on cAMP-agarose permits recovery of a protein complex consisting of the 90-kDa AKAP, RII, PP2B, and PKCzeta . With the use of small-particle flow cytometry, RII and PKCzeta were localized to an identical population of endosomes, suggesting that these proteins are components of an endosomal multiprotein complex. 32P-labeled aquaporin-2 (AQP2) present in these PKA-phosphorylated endosomes was dephosphorylated in vitro by either addition of exogenous PP2B or by an endogenous endosomal phosphatase that was inhibited by the PP2B inhibitors EDTA and the cyclophilin-cyclosporin A complex. We conclude that IMCD heavy endosomes possess an AKAP multiprotein-signaling complex similar to that described previously in hippocampal neurons. This signaling complex potentially mediates the phosphorylation of AQP2 to regulate its trafficking into the IMCD apical membrane. In addition, the PP2B component of the AKAP-signaling complex could also dephosphorylate AQP2 in vivo.

kidney; water channel; adenosine 3',5'-cyclic monophosphate; protein phosphatase 2B; aquaporin-2; A kinase anchoring protein

* I. Jo and D. T. Ward contributed equally to this work.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
L. A. Piggott, A. L. Bauman, J. D. Scott, and C. W. Dessauer
The A-kinase anchoring protein Yotiao binds and regulates adenylyl cyclase in brain
PNAS, September 16, 2008; 105(37): 13835 - 13840.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Y.-J. Lee, H.-J. Choi, J.-S. Lim, J.-H. Earm, B.-H. Lee, I.-S. Kim, J. Frokiaer, S. Nielsen, and T.-H. Kwon
A novel method of ligand peptidomics to identify peptide ligands binding to AQP2-expressing plasma membranes and intracellular vesicles of rat kidney
Am J Physiol Renal Physiol, July 1, 2008; 295(1): F300 - F309.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
P. Uawithya, T. Pisitkun, B. E. Ruttenberg, and M. A. Knepper
Transcriptional profiling of native inner medullary collecting duct cells from rat kidney
Physiol Genomics, January 17, 2008; 32(2): 229 - 253.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
L. J. Martin, H. Chen, X. Liao, H. Allayee, D. M. Shih, G. S. Lee, D. N. Hovland Jr, W. A. Robbins, K. Carnes, R. A. Hess, et al.
FK506, a Calcineurin Inhibitor, Prevents Cadmium-Induced Testicular Toxicity in Mice
Toxicol. Sci., December 1, 2007; 100(2): 474 - 485.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
A. K. Ahrabi, S. Terryn, G. Valenti, N. Caron, C. Serradeil-Le Gal, D. Raufaste, S. Nielsen, S. Horie, J.-M. Verbavatz, and O. Devuyst
PKD1 Haploinsufficiency Causes a Syndrome of Inappropriate Antidiuresis in Mice
J. Am. Soc. Nephrol., June 1, 2007; 18(6): 1740 - 1753.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S.-Z. Li, B. W. McDill, P. A. Kovach, L. Ding, W. Y. Go, S. N. Ho, and F. Chen
Calcineurin-NFATc signaling pathway regulates AQP2 expression in response to calcium signals and osmotic stress
Am J Physiol Cell Physiol, May 1, 2007; 292(5): C1606 - C1616.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. D. Hoffert, J. Nielsen, M.-J. Yu, T. Pisitkun, S. M. Schleicher, S. Nielsen, and M. A. Knepper
Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct
Am J Physiol Renal Physiol, February 1, 2007; 292(2): F691 - F700.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. L. Gooch, R. L. Guler, J. L. Barnes, and J. J. Toro
Loss of calcineurin A{alpha} results in altered trafficking of AQP2 and in nephrogenic diabetes insipidus
J. Cell Sci., June 15, 2006; 119(12): 2468 - 2476.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
T. Pisitkun, J. Bieniek, D. Tchapyjnikov, G. Wang, W. W. Wu, R.-F. Shen, and M. A. Knepper
High-throughput identification of IMCD proteins using LC-MS/MS
Physiol Genomics, April 13, 2006; 25(2): 263 - 276.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. Yao, D.-Y. Huang, I. L. Pfaff, X. Nie, M. Leitges, and V. Vallon
Evidence for a role of protein kinase C-{alpha} in urine concentration
Am J Physiol Renal Physiol, August 1, 2004; 287(2): F299 - F304.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online