| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
cotransport and associated gene
products in mouse DCT
1 Department of Anatomy and 3 Franz Volhard Clinic, Medical Faculty of the Charité, Humboldt University, 13353 Berlin, Germany; and 2 Department of Internal Medicine, Oregon Health Sciences University, Portland, Oregon 97201
First published August 8, 2001; 10.1152/ajprenal.00148.2001.
The
mammalian distal nephron develops a complex assembly of specialized cell types to accomplish the fine adjustment of urinary electrolyte composition. The epithelia of the distal convoluted tubule
(DCT), the connecting tubule (CNT), and the cortical collecting duct
(CCD) show an axial structural heterogeneity that has been functionally
elucidated by the localization of proteins involved in transepithelial
ion transport. We compared the distribution of the thiazide-sensitive
Na+-Cl
cotransporter (TSC), basolateral
Na+/Ca2+ exchanger (Na/Ca), cytosolic
calcium-binding proteins calbindin D28K and parvalbumin,
and the key enzyme for selective aldosterone actions,
11
-hydroxysteroid-dehydrogenase 2 (11HSD2), in the distal convolutions of the mouse. In the mouse, as opposed to the rat, we
found no clear subsegmentation of the DCT into a proximal (DCT1) and a
distal (DCT2) portion. The TSC was expressed along the entire DCT. Na/Ca and calbindin D28K were similarly expressed
along most of the DCT, with minor exceptions in the initial portion of
the DCT. Both were also present in the CNT. Parvalbumin was found in
the entire DCT, with an occasional absence from short end portions of
the DCT, and was not present in CNT. 11HSD2 was predominantly located
in the CNT and CCD. Short end portions of DCT only occasionally showed
the 11HSD2 signal. We also observed an overlap of 11HSD2 immunoreactivity and mRNA staining. Our observations will have implications in understanding the physiological effects of gene disruption and targeting experiments in the mouse.
distal convoluted tubule; connecting tubule; sodium-calcium
exchanger; calbindin; parvalbumin; 11-hydroxysteroid-dehydrogenase
type 2
This article has been cited by other articles:
|
|
 |
|
  G. Gamba The thiazide-sensitive Na+-Cl- cotransporter: molecular biology, functional properties, and regulation by WNKs Am J Physiol Renal Physiol, October 1, 2009; 297(4): F838 - F848. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. V. Hofmeister, R. A. Fenton, and J. Praetorius Fluorescence isolation of mouse late distal convoluted tubules and connecting tubules: effects of vasopressin and vitamin D3 on Ca2+ signaling Am J Physiol Renal Physiol, January 1, 2009; 296(1): F194 - F203. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Bailey, J. M. Paterson, P. W.F. Hadoke, N. Wrobel, C. O.C. Bellamy, D. G. Brownstein, J. R. Seckl, and J. J. Mullins A Switch in the Mechanism of Hypertension in the Syndrome of Apparent Mineralocorticoid Excess J. Am. Soc. Nephrol., January 1, 2008; 19(1): 47 - 58. [Full Text] [PDF]
|
|
|
|
|
|
P. R. Grimm, R. M. Foutz, R. Brenner, and S. C. Sansom Identification and localization of BK-beta subunits in the distal nephron of the mouse kidney Am J Physiol Renal Physiol, July 1, 2007; 293(1): F350 - F359. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Jiang, W. B. Ferguson, and J.-B. Peng WNK4 enhances TRPV5-mediated calcium transport: potential role in hypercalciuria of familial hyperkalemic hypertension caused by gene mutation of WNK4 Am J Physiol Renal Physiol, February 1, 2007; 292(2): F545 - F554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Naray-Fejes-Toth and G. Fejes-Toth Novel mouse strain with Cre recombinase in 11beta-hydroxysteroid dehydrogenase-2-expressing cells Am J Physiol Renal Physiol, January 1, 2007; 292(1): F486 - F494. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-T. Chen, J. J. Tu, J. Kao, X. K. Zhou, and M. Mazumdar Messenger RNA Expression Ratios among Four Genes Predict Subtypes of Renal Cell Carcinoma and Distinguish Oncocytoma from Carcinoma Clin. Cancer Res., September 15, 2005; 11(18): 6558 - 6566. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Gamba Molecular Physiology and Pathophysiology of Electroneutral Cation-Chloride Cotransporters Physiol Rev, April 1, 2005; 85(2): 423 - 493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Pluznick, P. Wei, P. R. Grimm, and S. C. Sansom BK-{beta}1 subunit: immunolocalization in the mammalian connecting tubule and its role in the kaliuretic response to volume expansion Am J Physiol Renal Physiol, April 1, 2005; 288(4): F846 - F854. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bachmann, K. Mutig, J. Bates, P. Welker, B. Geist, V. Gross, F. C. Luft, N. Alenina, M. Bader, B. J. Thiele, et al. Renal effects of Tamm-Horsfall protein (uromodulin) deficiency in mice Am J Physiol Renal Physiol, March 1, 2005; 288(3): F559 - F567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Nissant, S. Lourdel, S. Baillet, M. Paulais, P. Marvao, J. Teulon, and M. Imbert-Teboul Heterogeneous distribution of chloride channels along the distal convoluted tubule probed by single-cell RT-PCR and patch clamp Am J Physiol Renal Physiol, December 1, 2004; 287(6): F1233 - F1243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Loffing, V. Vallon, D. Loffing-Cueni, F. Aregger, K. Richter, L. Pietri, M. Bloch-Faure, J. G.J. Hoenderop, G. E. Shull, P. Meneton, et al. Altered Renal Distal Tubule Structure and Renal Na+ and Ca2+ Handling in a Mouse Model for Gitelman's Syndrome J. Am. Soc. Nephrol., September 1, 2004; 15(9): 2276 - 2288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Tian, M. Salanova, H. Xu, J. N. Lindsley, T. T. Oyama, S. Anderson, S. Bachmann, and D. M. Cohen Renal expression of osmotically responsive cation channel TRPV4 is restricted to water-impermeant nephron segments Am J Physiol Renal Physiol, July 1, 2004; 287(1): F17 - F24. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Y. Li, C. L. Huey, and A. S. L. Yu Expression of claudin-7 and -8 along the mouse nephron Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1063 - F1071. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Gonzalez-Nunez, M. Morales-Ruiz, A. Leivas, S. C. Hebert, and E. Poch In vitro characterization of aldosterone and cAMP effects in mouse distal convoluted tubule cells Am J Physiol Renal Physiol, May 1, 2004; 286(5): F936 - F944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. W. Diepens, E. den Dekker, M. Bens, A. F. Weidema, A. Vandewalle, R. J. M. Bindels, and J. G. J. Hoenderop Characterization of a murine renal distal convoluted tubule cell line for the study of transcellular calcium transport Am J Physiol Renal Physiol, March 1, 2004; 286(3): F483 - F489. [Abstract] [Full Text]
|
|
|
|
|
|
V. Campean, F. Theilig, A. Paliege, M. Breyer, and S. Bachmann Key enzymes for renal prostaglandin synthesis: site-specific expression in rodent kidney (rat, mouse) Am J Physiol Renal Physiol, July 1, 2003; 285(1): F19 - F32. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. K. Stricklett, D. Taylor, R. D. Nelson, and D. E. Kohan Thick ascending limb-specific expression of Cre recombinase Am J Physiol Renal Physiol, July 1, 2003; 285(1): F33 - F39. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Saito, Y. Guan, Z. Qi, L. S. Davis, M. Komhoff, Y. Sugimoto, S. Narumiya, R. M. Breyer, and M. D. Breyer Expression of the prostaglandin F receptor (FP) gene along the mouse genitourinary tract Am J Physiol Renal Physiol, June 1, 2003; 284(6): F1164 - F1170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Loffing and B. Kaissling Sodium and calcium transport pathways along the mammalian distal nephron: from rabbit to human Am J Physiol Renal Physiol, April 1, 2003; 284(4): F628 - F643. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Magyar, K. E. White, R. Rojas, G. Apodaca, and P. A. Friedman Plasma membrane Ca2+-ATPase and NCX1 Na+/Ca2+ exchanger expression in distal convoluted tubule cells Am J Physiol Renal Physiol, July 1, 2002; 283(1): F29 - F40. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Wade Distribution of transporters along the mouse distal nephron: something old, something borrowed, something new Am J Physiol Renal Physiol, December 1, 2001; 281(6): F1019 - F1020. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
