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Am J Physiol Renal Physiol 282: F245-F255, 2002. First published August 15, 2001; doi:10.1152/ajprenal.0038.2001
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Vol. 282, Issue 2, F245-F255, February 2002

P2 receptor-mediated afferent arteriolar vasoconstriction during calcium blockade

Edward W. Inscho and Anthony K. Cook

Department of Physiology, Tulane University School of Medicine, New Orleans, Louisiana 70112; and Department of Physiology, Medical College of Georgia, Augusta, Georgia 30912-3000

Experiments were performed to determine the role of L-type calcium channels on the afferent arteriolar vasoconstrictor response to ATP and UTP. With the use of the blood-perfused juxtamedullary nephron technique, kidneys were perfused at 110 mmHg and the responses of arterioles to alpha ,beta -methylene ATP, ATP, and UTP were determined before and during calcium channel blockade with diltiazem. alpha ,beta -Methylene ATP (1.0 µM) decreased arteriolar diameter by 8 ± 1% under control conditions. This response was abolished during calcium channel blockade. In contrast, 10 µM UTP reduced afferent arteriolar diameter to a similar degree before (20 ± 4%) and during (14 ± 4%) diltiazem treatment. Additionally, diltiazem completely prevented the vasoconstriction normally observed with ATP concentrations below 10 µM and attenuated the response obtained with 10 µM ATP. These data demonstrate that L-type calcium channels play a significant role in the vasoconstrictor influences of alpha ,beta -methylene ATP and ATP but not UTP. The data also suggest that other calcium influx pathways may participate in the vasoconstrictor response evoked by P2 receptor activation. These observations support previous findings that UTP-mediated elevation of intracellular calcium concentration in preglomerular vascular smooth muscle cells relies primarily on calcium release from intracellular pools, whereas ATP-mediated responses involve both voltage-dependent calcium influx, through L-type calcium channels, and the release of calcium from intracellular stores. These results support the argument that P2X and P2Y receptors influence the diameter of afferent arterioles through activation of disparate signal transduction mechanisms.

afferent arterioles; calcium channels; cytosolic calcium; renal microcirculation; P2X receptors; P2Y receptors; adenosine 5'-triphosphate; uridine 5'-triphosphate; alpha ,beta -methylene adenosine 5'-triphosphate; cadmium


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