| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Cell Physiology, University Medical Center St. Radboud, Nijmegen 6500 HB, The Netherlands; 2 Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205; 3 Brooklyn College, Brooklyn, New York 11210; and 4 Department of Cellular and Molecular Physiology, Yale University, New Haven, Connecticut 06520
Renal regulation of mammalian water homeostasis is mediated by the aquaporin-1 (AQP1) water channel, which is expressed in the apical and basolateral membranes of proximal tubules and descending limbs of Henle, and aquaporin-2 (AQP2), which is redistributed from intracellular vesicles to the apical membrane (AM) of collecting duct cells with vasopressin. In transfected Madin-Darby canine kidney cells, AQP1 and AQP2 are regulated similarly, which indicates that routing elements reside in their primary sequences. We studied the role of the AQP2 COOH terminus in apical routing and AQP2 shuttling. An AQP1 chimera (AQP1 with an AQP2 tail: AQP1/2-N220) was located only in the AM independent of forskolin treatment. Forskolin increased the apical expression of AQP1 and AQP1/2-N220 less than twofold; that of AQP2 increased more than fourfold with concomitant changes in osmotic water permeabilities. The dimeric AQP2 tail coupled to placental alkaline phosphatase (AQP2-Plap) was retained in intracellular vesicles different from those of homotetrameric wild-type AQP2; the same protein without the AQP2 tail (TMR-Plap) was only expressed in the AM. The study shows that the AQP2 COOH tail is necessary but not sufficient for routing to the AM and suggests that other parts of AQP2 are needed for AQP2 accumulation in intracellular vesicles.
routing; shuttling; Madin-Darby canine kidney cells; vasopressin
This article has been cited by other articles:
|
|
 |
|
  J. H. Robben, M. Sze, N. V. Knoers, P. Eggert, P. Deen, and D. Muller Relief of Nocturnal Enuresis by Desmopressin Is Kidney and Vasopressin Type 2 Receptor Independent J. Am. Soc. Nephrol., May 1, 2007; 18(5): 1534 - 1539. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. P. Shi, X. R. Cao, J. Qu, K. A. Volk, P. Kirby, R. A. Williamson, J. B. Stokes, and B. Yang Nephrogenic diabetes insipidus in mice caused by deleting COOH-terminal tail of aquaporin-2 Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1334 - F1344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Robben, M. Sze, N. V. A. M. Knoers, and P. M. T. Deen Functional rescue of vasopressin V2 receptor mutants in MDCK cells by pharmacochaperones: relevance to therapy of nephrogenic diabetes insipidus Am J Physiol Renal Physiol, January 1, 2007; 292(1): F253 - F260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Li, S. Shaw, E.-J. Kamsteeg, A. Vandewalle, and P. M.T. Deen Development of Lithium-Induced Nephrogenic Diabetes Insipidus Is Dissociated from Adenylyl Cyclase Activity J. Am. Soc. Nephrol., April 1, 2006; 17(4): 1063 - 1072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. de Mattia, P. J.M. Savelkoul, E.-J. Kamsteeg, I. B.M. Konings, P. van der Sluijs, R. Mallmann, A. Oksche, and P. M.T. Deen Lack of Arginine Vasopressin-Induced Phosphorylation of Aquaporin-2 Mutant AQP2-R254L Explains Dominant Nephrogenic Diabetes Insipidus J. Am. Soc. Nephrol., October 1, 2005; 16(10): 2872 - 2880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Robben, N. V. A. M. Knoers, and P. M. T. Deen Characterization of vasopressin V2 receptor mutants in nephrogenic diabetes insipidus in a polarized cell model Am J Physiol Renal Physiol, August 1, 2005; 289(2): F265 - F272. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. de Mattia, P. J.M. Savelkoul, D. G. Bichet, E.-J. Kamsteeg, I. B.M. Konings, N. Marr, M.-F. Arthus, M. Lonergan, C. H. van Os, P. van der Sluijs, et al. A novel mechanism in recessive nephrogenic diabetes insipidus: wild-type aquaporin-2 rescues the apical membrane expression of intracellularly retained AQP2-P262L Hum. Mol. Genet., December 15, 2004; 13(24): 3045 - 3056. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. W. M. van Balkom, M. P. J. Graat, M. van Raak, E. Hofman, P. van der Sluijs, and P. M. T. Deen Role of cytoplasmic termini in sorting and shuttling of the aquaporin-2 water channel Am J Physiol Cell Physiol, February 1, 2004; 286(2): C372 - C379. [Abstract] [Full Text]
|
|
|
|
 |
|
 E.-J. Kamsteeg, D. G. Bichet, I. B.M. Konings, H. Nivet, M. Lonergan, M.-F. Arthus, C. H. van Os, and P. M.T. Deen Reversed polarized delivery of an aquaporin-2 mutant causes dominant nephrogenic diabetes insipidus J. Cell Biol., December 8, 2003; 163(5): 1099 - 1109. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Caplan How megalin finds its way: identification of a novel apical sorting motif. Focus on "Identification of an apical sorting determinant in the cytoplasmic tail of megalin" Am J Physiol Cell Physiol, May 1, 2003; 284(5): C1101 - C1104. [Full Text] [PDF]
|
|
|
|
|
|
D. Brown The ins and outs of aquaporin-2 trafficking Am J Physiol Renal Physiol, May 1, 2003; 284(5): F893 - F901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Marr, D. G. Bichet, S. Hoefs, P. J. M. Savelkoul, I. B. M. Konings, F. de Mattia, M. P. J. Graat, M.-F. Arthus, M. Lonergan, T. M. Fujiwara, et al. Cell-Biologic and Functional Analyses of Five New Aquaporin-2 Missense Mutations that Cause Recessive Nephrogenic Diabetes Insipidus J. Am. Soc. Nephrol., September 1, 2002; 13(9): 2267 - 2277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Marr, D. G Bichet, M. Lonergan, M.-F. Arthus, N. Jeck, H. W. Seyberth, W. Rosenthal, C. H. van Os, A. Oksche, and P. M. T. Deen Heteroligomerization of an Aquaporin-2 mutant with wild-type Aquaporin-2 and their misrouting to late endosomes/lysosomes explains dominant nephrogenic diabetes insipidus Hum. Mol. Genet., April 1, 2002; 11(7): 779 - 789. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
