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-subunit in rat
kidney
Laboratory of Membrane Biology, Neuroscience Center, Massachusetts General Hospital, Charlestown, Massachusetts 02129
Renal Na+-K+-ATPase is associated
with the 
-subunit (FXYD2), a single-span membrane protein that
modifies ATPase properties. There are two splice variants with
different amino termini, a and b. Both
were found in the inner stripe of the outer medulla in the thick
ascending limb. Coimmunoprecipitation with each other and the
-subunit indicated that they were associated in macromolecular complexes. Association was controlled by ligands that affect
Na+-K+-ATPase conformation. In the cortex, the
proportion of the b-subunit was markedly lower, and the
a-subunit predominated in isolated proximal tubule
cells. By immunofluorescence, the b-subunit was detected
in the superficial cortex only in the distal convoluted tubule and
connecting tubule, which are rich in
Na+-K+-ATPase but comprise a minor fraction of
cortex mass. In the outer stripe of the outer medulla and for a short
distance in the deep cortex, the thick ascending limb predominantly
expressed the b-subunit. Because different mechanisms
maintain and regulate Na+ homeostasis in different nephron
segments, the splice forms of the -subunit may have evolved to
control the renal Na+ pump through pump properties, gene
expression, or both.
sodium pump; nephron; immunofluorescence; confocal microscopy
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