AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 282: F512-F522, 2002; doi:10.1152/ajprenal.00111.2000
0363-6127/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (18)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Khundmiri, S. J.
Right arrow Articles by Lederer, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Khundmiri, S. J.
Right arrow Articles by Lederer, E.
Vol. 282, Issue 3, F512-F522, March 2002

PTH and DA regulate Na-K ATPase through divergent pathways

Syed Jalal Khundmiri and Eleanor Lederer2

2 Veterans Administration and Department of Medicine1,2, University of Louisville, School of Medicine, Louisville, Kentucky 40202

Parathyroid hormone (PTH) and dopamine (DA) inhibit Na-K ATPase activity and sodium-phosphate cotransport in proximal tubular cells. We previously showed that PTH and DA inhibit phosphate transport in opossum kidney (OK) cells through different signaling pathways. Therefore, we hypothesized that PTH and DA also inhibit Na-K ATPase through divergent pathways. We measured PTH and DA inhibition of Na-K ATPase activity in the presence of inhibitors of signaling pathways. PTH and DA inhibited Na-K ATPase in a biphasic manner, the early inhibition through protein kinase C (PKC)- and phospholipase A2 (PLA2)-dependent pathways and the late inhibition through protein kinase A- and PLA2-dependent pathways. Inhibition of extracellular signal-regulated kinase (ERK) activation blocked early and late inhibition of Na-K ATPase by PTH but not by DA. Pertussis toxin blocked early and late inhibition by DA but not by PTH. Treatment with DA, but not PTH, resulted in an early downregulation of basolateral membrane expression of the alpha -subunit, whereas total cellular expression remained constant for both agonists. We conclude that PTH and DA regulate Na-K ATPase by different mechanisms through activation of divergent pathways.

protein kinase A; protein kinase C; phospholipase A2; opossum kidney cells; extracellular signal-regulated kinase


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
S. J. Khundmiri, M. Ameen, N. A. Delamere, and E. D. Lederer
PTH-mediated regulation of Na+-K+-ATPase requires Src kinase-dependent ERK phosphorylation
Am J Physiol Renal Physiol, August 1, 2008; 295(2): F426 - F437.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. Zeng, I. Armando, Y. Luo, G. M. Eisner, R. A. Felder, and P. A. Jose
Dysregulation of dopamine-dependent mechanisms as a determinant of hypertension: studies in dopamine receptor knockout mice
Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H551 - H569.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
T. Kimura, P. B. Allen, A. C. Nairn, and M. J. Caplan
Arrestins and Spinophilin Competitively Regulate Na+,K+-ATPase Trafficking through Association with a Large Cytoplasmic Loop of the Na+,K+-ATPase
Mol. Biol. Cell, November 1, 2007; 18(11): 4508 - 4518.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. Kirchheimer, C. F. Mendez, A. Acquier, and S. Nowicki
Role of 20-HETE in D1/D2 dopamine receptor synergism resulting in the inhibition of Na+-K+-ATPase activity in the proximal tubule
Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1435 - F1442.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. Plourde and S. P. Soltoff
Ouabain potentiates the activation of ERK1/2 by carbachol in parotid gland epithelial cells; inhibition of ERK1/2 reduces Na+-K+-ATPase activity
Am J Physiol Cell Physiol, March 1, 2006; 290(3): C702 - C710.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
S. J. Khundmiri, E. J. Weinman, D. Steplock, J. Cole, A. Ahmad, P. D. Baumann, M. Barati, M. J. Rane, and E. Lederer
Parathyroid Hormone Regulation of Na+,K+-ATPase Requires the PDZ 1 Domain of Sodium Hydrogen Exchanger Regulatory Factor-1 in Opossum Kidney Cells
J. Am. Soc. Nephrol., September 1, 2005; 16(9): 2598 - 2607.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
C. Zeng, H. Sanada, H. Watanabe, G. M. Eisner, R. A. Felder, and P. A. Jose
Functional genomics of the dopaminergic system in hypertension
Physiol Genomics, November 17, 2004; 19(3): 233 - 246.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. D. Bozulic, W. L. Dean, and N. A. Delamere
The influence of Lyn kinase on Na,K-ATPase in porcine lens epithelium
Am J Physiol Cell Physiol, January 1, 2004; 286(1): C90 - C96.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
E. D. Lederer, S. J. Khundmiri, and E. J. Weinman
Role of NHERF-1 in Regulation of the Activity of Na-K ATPase and Sodium-Phosphate Co-transport in Epithelial Cells
J. Am. Soc. Nephrol., July 1, 2003; 14(7): 1711 - 1719.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
M. Levi
Role of PDZ Domain-Containing Proteins and ERM Proteins in Regulation of Renal Function and Dysfunction
J. Am. Soc. Nephrol., July 1, 2003; 14(7): 1949 - 1951.
[Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
T. Hussain and M. F. Lokhandwala
Renal Dopamine Receptors and Hypertension
Experimental Biology and Medicine, February 1, 2003; 228(2): 134 - 142.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online