AJP - Renal Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 282: F599-F607, 2002. First published November 20, 2001; doi:10.1152/ajprenal.00284.2001
0363-6127/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/4/F599    most recent
00284.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vázquez, N.
Right arrow Articles by Gamba, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vázquez, N.
Right arrow Articles by Gamba, G.
Vol. 282, Issue 4, F599-F607, April 2002

Functional differences between flounder and rat thiazide-sensitive Na-Cl cotransporter

Norma Vázquez1, Adriana Monroy1, Elisa Dorantes1, Rosario A. Muñoz-Clares2, and Gerardo Gamba1

1 Molecular Physiology Unit, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán and Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Tlalpan 14000, Mexico City; and 2 Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, Coyoacán 04510, Mexico City, Mexico

The purpose of the present study was to determine the major functional, pharmacological, and regulatory properties of the flounder thiazide-sensitive Na-Cl cotransporter (flTSC) to make a direct comparison with our recent characterization of the rat TSC (rTSC; Monroy A, Plata C, Hebert SC, and Gamba G. Am J Physiol Renal Physiol 279: F161-F169, 2000). When expressed in Xenopus laevis oocytes, flTSC exhibits lower affinity for Na+ than for Cl-, with apparent Michaelis-Menten constant (Km) values of 58.2 ± 7.1 and 22.1 ± 4.2 mM, respectively. These Km values are significantly higher than those observed in rTSC. The Na+ and Cl- affinities decreased when the concentration of the counterion was lowered, suggesting that the binding of one ion increases the affinity of the transporter for the other. The effect of several thiazides on flTSC function was biphasic. Low concentrations of thiazides (10-9 to 10-7 M) resulted in activation of the cotransporter, whereas higher concentrations (10-6 to 10-4 M) were inhibitory. In rTSC, this biphasic effect was observed only with chlorthalidone. The affinity for thiazides in flTSC was lower than in rTSC, but the affinity in flTSC was not affected by the Na+ or the Cl- concentration in the uptake medium. In addition to thiazides, flTSC and rTSC were inhibited by Hg2+, with an apparent higher affinity for rTSC. Finally, flTSC function was decreased by activation of protein kinase C with phorbol esters and by hypertonicity. In summary, we have found significant regulatory, kinetic, and pharmacological differences between flTSC and rTSC orthologues.

metolazone; distal tubule; osmolarity; salt reabsorption; thiazide-sensitive sodium-chloride cotransporter


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. Esaki, K. Hoshijima, S. Kobayashi, H. Fukuda, K. Kawakami, and S. Hirose
Visualization in zebrafish larvae of Na+ uptake in mitochondria-rich cells whose differentiation is dependent on foxi3a
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2007; 292(1): R470 - R480.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Moreno, P. S. Cristobal, M. Rivera, N. Vazquez, N. A. Bobadilla, and G. Gamba
Affinity-defining Domains in the Na-Cl Cotransporter: A DIFFERENT LOCATION FOR Cl- AND THIAZIDE BINDING
J. Biol. Chem., June 23, 2006; 281(25): 17266 - 17275.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. M. Weinstein
A mathematical model of rat distal convoluted tubule. I. Cotransporter function in early DCT
Am J Physiol Renal Physiol, October 1, 2005; 289(4): F699 - F720.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
G. Gamba
Molecular Physiology and Pathophysiology of Electroneutral Cation-Chloride Cotransporters
Physiol Rev, April 1, 2005; 85(2): 423 - 493.
[Abstract] [Full Text] [PDF]

Home page
 Journal of Renin-Angiotensin-Aldosterone SystemHome page
A. D Hughes
How do thiazide and thiazide-like diuretics lower blood pressure?
Journal of Renin-Angiotensin-Aldosterone System, December 1, 2004; 5(4): 155 - 160.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
E. Sabath, P. Meade, J. Berkman, P. d. l. Heros, E. Moreno, N. A. Bobadilla, N. Vazquez, D. H. Ellison, and G. Gamba
Pathophysiology of functional mutations of the thiazide-sensitive Na-Cl cotransporter in Gitelman disease
Am J Physiol Renal Physiol, August 1, 2004; 287(2): F195 - F203.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
J. C. de Jong, P. H.G.M. Willems, L. P.W.J. van den Heuvel, N. V.A.M. Knoers, and R. J.M. Bindels
Functional Expression of the Human Thiazide-Sensitive NaCl Cotransporter in Madin-Darby Canine Kidney Cells
J. Am. Soc. Nephrol., October 1, 2003; 14(10): 2428 - 2435.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
R. S. Hoover, E. Poch, A. Monroy, N. Vazquez, T. Nishio, G. Gamba, and S. C. Hebert
N-Glycosylation at Two Sites Critically Alters Thiazide Binding and Activity of the Rat Thiazide-sensitive Na+:Cl- Cotransporter
J. Am. Soc. Nephrol., February 1, 2003; 14(2): 271 - 282.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online