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Am J Physiol Renal Physiol 282: F649-F654, 2002; doi:10.1152/ajprenal.00291.2000
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Vol. 282, Issue 4, F649-F654, April 2002

Expression of isoforms of the Na+/H+ exchanger in M-1 mouse cortical collecting duct cells

C. Hill, A. N. Giesberts, and S. J. White

Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom

Na+/H+ exchanger (NHE) proteins perform a variety of functions in the kidney and are differentially distributed among nephron segments. The purpose of this study was to identify NHE isoforms in murine M-1 cells as a model of cortical collecting duct principal cells. It was found that mRNAs corresponding to NHE1, NHE2, and NHE4 are expressed in M-1 cells. NHE-dependent regulation of intracellular pH (pHi) was investigated in the absence of extracellular HCO<UP><SUB>3</SUB><SUP>−</SUP></UP>. Application of a 20 mM NH4Cl pulse resulted in a reversible intracellular acidification from which recovery was partially inhibited by application of 1 mM amiloride to either the apical or the basolateral membranes and was abolished when amiloride was applied to both sides of the monolayers, which suggests that NHEs are expressed in both the apical and the basolateral cell membranes of M-1 cells. The purinergic agonists ATP and benzoylbenzoyl-ATP caused a reduction of pHi when applied to the apical membrane, which suggests pHi may be influenced by extracellular nucleotides in the luminal fluid of the cortical collecting duct.

mouse; cortical collecting duct principal cells; amiloride; adenosine 5'-triphosphate intracellular pH; sodium/hydrogen exchanger


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