| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709; and 2 Institut fur Pharmazeutische Technologie und Biopharmazie, INF 366, D-69120 Heidelberg, Germany
We used confocal microscopy and quantitative image analysis to follow the movement of the fluorescent organic anion fluorescein (FL) from bath to cell and cell to blood vessel in intact rat lateral choroid plexus. FL accumulation in epithelial cells and underlying vessels was rapid, concentrative, and reduced by other organic anions. At steady state, cell fluorescence exceeded bath fluorescence by a factor of 3-5, and vessel fluorescence exceeded cell fluorescence by a factor of ~2. In cells, FL distributed between diffuse and punctate compartments. Cell and vessel accumulation of FL decreased when metabolism was inhibited by KCN, when bath Na+ was reduced from 130 to 26 mM, and when the Na+ gradient was collapsed with ouabain. Cell and vessel accumulation increased by >50% when 1-10 µM glutarate was added to the bath. Finally, transport of FL and carboxyfluorescein (generated intracellularly from carboxyfluorescein diacetate) from cell to blood vessel was greatly diminished when medium K+ concentration ([K+]) was increased 10-fold. These results 1) validate a new approach to the study of choroid plexus function, and 2) indicate a two-step mechanism for transepithelial organic anion transport: indirect coupling of uptake to Na+ at the apical membrane and electrical potential-driven efflux at the basolateral membrane.
transepithelial transport; potential-driven efflux; blood-brain barrier; 2,4-dichlorophenoxyacetic acid; fluorescein
This article has been cited by other articles:
|
|
 |
|
  V. Reichel, D. S. Miller, and G. Fricker Texas Red transport across rat and dogfish shark (Squalus acanthias) choroid plexus Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2008; 295(4): R1311 - R1319. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. Baehr, G. Fricker, and D. S. Miller Fluorescein-methotrexate transport in dogfish shark (Squalus acanthias) choroid plexus Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2006; 291(2): R464 - R472. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Breen, D. B. Sykes, C. Baehr, G. Fricker, and D. S. Miller Fluorescein-methotrexate transport in rat choroid plexus analyzed using confocal microscopy Am J Physiol Renal Physiol, September 1, 2004; 287(3): F562 - F569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Imaoka, H. Kusuhara, S. Adachi-Akahane, M. Hasegawa, N. Morita, H. Endou, and Y. Sugiyama The Renal-Specific Transporter Mediates Facilitative Transport of Organic Anions at the Brush Border Membrane of Mouse Renal Tubules J. Am. Soc. Nephrol., August 1, 2004; 15(8): 2012 - 2022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Sykes, D. H. Sweet, S. Lowes, S. K. Nigam, J. B. Pritchard, and D. S. Miller Organic anion transport in choroid plexus from wild-type and organic anion transporter 3 (Slc22a8)-null mice Am J Physiol Renal Physiol, May 1, 2004; 286(5): F972 - F978. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. H. Sweet, D. S. Miller, J. B. Pritchard, Y. Fujiwara, D. R. Beier, and S. K. Nigam Impaired Organic Anion Transport in Kidney and Choroid Plexus of Organic Anion Transporter 3 (Oat3 (Slc22a8)) Knockout Mice J. Biol. Chem., July 19, 2002; 277(30): 26934 - 26943. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
