AJP - Renal Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 282: F1034-F1042, 2002. First published January 29, 2002; doi:10.1152/ajprenal.00136.2001
0363-6127/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/6/F1034    most recent
00136.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (8)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Spector, D. A.
Right arrow Articles by Weinman, E. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Spector, D. A.
Right arrow Articles by Weinman, E. J.
Vol. 282, Issue 6, F1034-F1042, June 2002

Expression, localization, and regulation of aquaporin-1 to -3 in rat urothelia

David A. Spector1, James B. Wade2, Russell Dillow1, Deborah A. Steplock3, and Edward J. Weinman3,4

1 Division of Renal Medicine, The Johns Hopkins Bayview Medical Center, Baltimore 21224; and Departments of 2 Physiology and 3 Medicine, University of Maryland School of Medicine, and 4 Department of Veterans Affairs, Baltimore, Maryland 21201

Although mammalian urothelia are generally considered impermeable to constituents of urine, in vivo studies in several species indicate urothelial transport of water and solutes under certain conditions. This study investigates the expression, localization, and regulation of aquaporin (AQP)-1, -2, and -3 in ureteral and bladder tissues in 48-h dehydrated and water-loaded female Wistar rats. Immunoblots of homogenates of whole ureter and bladder identified characteristic ~28- and 35- to 44-kDa bands for AQP-1, -2, and -3. AQP-1 was localized to capillary and arteriole endothelial cells, whereas AQP-2 and -3 circumferentially lined the epithelial cell membranes except for the apical membrane of the epithelial cells adjacent to the lumens of both ureter and bladder. AQP-2 was also present in epithelial cell cytoplasm. Dehydration resulted in 160-200% increases of AQP-3 signal and 24-49% increases of AQP-2 signal but no change in AQP-1 signal on immunoblots of homogenates of ureters and bladders. AQPs in genitourinary tract urothelia likely play a role in the regulation of epithelial cell volume and osmolality and may play a role in bulk water movement across urothelia.

Western blot analysis; immunocytochemistry; water transport


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
D. A. Spector, Q. Yang, L. Klopouh, J. Deng, E. J. Weinman, D. A. Steplock, R. Biswas, M. F. Brazie, J. Liu, and J. B. Wade
The ROMK potassium channel is present in mammalian urinary tract epithelia and muscle
Am J Physiol Renal Physiol, December 1, 2008; 295(6): F1658 - F1665.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
B. Hartman Bakken, L. G. Herrera M., R. M. Carroll, J. Ayala-Berdon, J. E. Schondube, and C. Martinez del Rio
A nectar-feeding mammal avoids body fluid disturbances by varying renal function
Am J Physiol Renal Physiol, December 1, 2008; 295(6): F1855 - F1863.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. A. Spector, Q. Yang, and J. B. Wade
High urea and creatinine concentrations and urea transporter B in mammalian urinary tract tissues
Am J Physiol Renal Physiol, January 1, 2007; 292(1): F467 - F474.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. Zhao, L. Bankir, L. Qian, D. Yang, and B. Yang
Urea and urine concentrating ability in mice lacking AQP1 and AQP3
Am J Physiol Renal Physiol, August 1, 2006; 291(2): F429 - F438.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. A. Spector, Q. Yang, J. Liu, and J. B. Wade
Expression, localization, and regulation of urea transporter B in rat urothelia
Am J Physiol Renal Physiol, July 1, 2004; 287(1): F102 - F108.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online