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Departments of 1 Medicine and 2 Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461
PGT is a broadly expressed transporter of
prostaglandins (PGs) and thromboxane that is energetically poised to
take up prostanoids across the plasma membrane. To gain insight into
the function of PGT, we generated mouse monoclonal antibody 20 against
a portion of putative extracellular loop 5 of rat PGT. Immunoblots of
endogenous PGT in rat kidney revealed a 65-kDa protein in a zonal
pattern corresponding to PG synthesis rates (papilla 
medulla > cortex). Immunocytochemically, PGT in rat kidneys was
expressed in glomerular endothelial and mesangial cells, arteriolar
endothelial and muscularis cells, principal cells of the collecting
duct, medullary interstitial cells, medullary vasa rectae endothelia,
and papillary surface epithelium. Proximal tubules, which are known to
take up and metabolize PGs, were negative. Immunoblotting and
immunocytochemistry revealed that rat platelets also express abundant
PGT. Coexpression of the PG synthesis apparatus (cyclooxygenase) and
PGT by the same cell suggests that prostanoids may undergo release and reuptake.
carrier proteins; biological transport; molecular cloning
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