AJP - Renal Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 282: F1129-F1139, 2002. First published January 8, 2002; doi:10.1152/ajprenal.00131.2001
0363-6127/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/6/F1129    most recent
00131.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (14)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, Y.
Right arrow Articles by Kershaw, D. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, Y.
Right arrow Articles by Kershaw, D. B.
Vol. 282, Issue 6, F1129-F1139, June 2002

PDZ domain-mediated interaction of rabbit podocalyxin and Na+/H+ exchange regulatory factor-2

Yong Li1, Jian Li1, Samuel W. Straight2, and David B. Kershaw1

Department of 1 Pediatrics and 2 Howard Hughes Medical Institute, University of Michigan, Ann Arbor, Michigan 48109-0676

The transmembrane sialoglycoprotein podocalyxin is thought to be essential in the fine interdigitating foot process structure of the podocyte. The intracellular COOH-terminal amino acids Asp-Thr-His-Leu (DTHL) of podocalyxin comprise a putative ligand for a type I PSD95-Dlg-zona occludens-1 (PDZ) domain. A 20-amino acid synthetic peptide containing this motif was used to screen a cDNA library, and clones of rabbit Na+/H+ exchange regulatory factor-2 (NHERF-2) were obtained. In vitro analysis demonstrated that each PDZ domain of NHERF-2 could bind podocalyxin independently. NHERF-2 coprecipitated from glomerular extracts with podocalyxin, and podocalyxin and NHERF-2 colocalized in the glomerular capillary loops, indicating that podocalyxin and NHERF-2 may interact in vivo. Podocalyxin peptide missing the terminal leucine (-DTHL) failed to interact with NHERF-2 in vitro. Podocalyxin localized to the apical membrane of transfected Madin-Darby canine kidney (MDCK) cells. However, mutant podocalyxin (missing a functional DTHL COOH-terminal motif) showed cytoplasmic and apical membrane localization in transfected cells and was also less stable at the apical membrane, as assessed by confocal microscopy and biotinylation studies. Mutant podocalyxin did lower the transepithelial resistance of MDCK cell monolayers, albeit to a lesser extent than full-length podocalyxin. We conclude that podocalyxin can interact with both PDZ domains of NHERF-2 and that this interaction requires the intact COOH terminus of podocalyxin, which is also responsible for the efficient apical localization of podocalyxin in transfected MDCK cells. These results suggest that the interaction of podocalyxin with NHERF-2 may function to efficiently retain podocalyxin at the apical surface of the podocyte and provide a mechanism linking podocalyxin to the actin cytoskeleton.

podocyte; sialomucin; E3KARP; PSD95-Dlg-zona occludens-1 domain


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
M. Donowitz and X. Li
Regulatory Binding Partners and Complexes of NHE3
Physiol Rev, July 1, 2007; 87(3): 825 - 872.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
C.-Y. Yu, J.-Y. Chen, Y.-Y. Lin, K.-F. Shen, W.-L. Lin, C.-L. Chien, M. B.A. ter Beest, and T.-S. Jou
A Bipartite Signal Regulates the Faithful Delivery of Apical Domain Marker Podocalyxin/Gp135
Mol. Biol. Cell, May 1, 2007; 18(5): 1710 - 1722.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
P. C. Tan, S. G.B. Furness, H. Merkens, S. Lin, M. L. McCoy, C. D. Roskelley, J. Kast, and K. M. McNagny
Na+/H+ Exchanger Regulatory Factor-1 Is a Hematopoietic Ligand for a Subset of the CD34 Family of Stem Cell Surface Proteins
Stem Cells, May 1, 2006; 24(5): 1150 - 1161.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Y. Harita, N. Miyauchi, T. Karasawa, K. Suzuki, G. D. Han, H. Koike, T. Igarashi, F. Shimizu, and H. Kawachi
Altered expression of junctional adhesion molecule 4 in injured podocytes
Am J Physiol Renal Physiol, February 1, 2006; 290(2): F335 - F344.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
H.-Y. Cheng, Y.-Y. Lin, C.-Y. Yu, J.-Y. Chen, K.-F. Shen, W.-L. Lin, H.-K. Liao, Y.-J. Chen, C.-H. Liu, V. F. Pang, et al.
Molecular Identification of Canine Podocalyxin-Like Protein 1 as a Renal Tubulogenic Regulator
J. Am. Soc. Nephrol., June 1, 2005; 16(6): 1612 - 1622.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
D. Meder, A. Shevchenko, K. Simons, and J. Fullekrug
Gp135/podocalyxin and NHERF-2 participate in the formation of a preapical domain during polarization of MDCK cells
J. Cell Biol., January 17, 2005; 168(2): 303 - 313.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
S. Shenolikar, J. W. Voltz, R. Cunningham, and E. J. Weinman
Regulation of Ion Transport by the NHERF Family of PDZ Proteins
Physiology, December 1, 2004; 19(6): 362 - 369.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
S. Schmieder, M. Nagai, R. A. Orlando, T. Takeda, and M. G. Farquhar
Podocalyxin Activates RhoA and Induces Actin Reorganization through NHERF1 and Ezrin in MDCK Cells
J. Am. Soc. Nephrol., September 1, 2004; 15(9): 2289 - 2298.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. B. Wade, J. Liu, R. A. Coleman, R. Cunningham, D. A. Steplock, W. Lee-Kwon, T. L. Pallone, S. Shenolikar, and E. J. Weinman
Localization and interaction of NHERF isoforms in the renal proximal tubule of the mouse
Am J Physiol Cell Physiol, December 1, 2003; 285(6): C1494 - C1503.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online