AJP - Renal Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 283: F124-F133, 2002. First published January 29, 2002; doi:10.1152/ajprenal.00367.2001
0363-6127/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/1/F124    most recent
00367.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, X.
Right arrow Articles by Wright, S. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, X.
Right arrow Articles by Wright, S. H.
Vol. 283, Issue 1, F124-F133, July 2002

Molecular cloning of rabbit organic cation transporter rbOCT2 and functional comparisons with rbOCT1

Xiaohong Zhang, Kristen K. Evans, and Stephen H. Wright

Department of Physiology, College of Medicine, University of Arizona, Tucson, Arizona 85724

Multiple organic cation transporters (OCTs) are present in rabbit kidney and may play different functional roles. We cloned rabbit OCT2 (rbOCT2) and compared its function with that of rabbit OCT1 (rbOCT1). In transiently transfected COS-7 cells, rbOCT1 and rbOCT2 mediated uptake of [3H]tetraethylammonium (TEA) with Kt values of 188 and 125 µM, respectively. n-Tetraalkylammonium compounds showed similar affinities for the two homologs, with IC50 values for inhibition of OCT1- and OCT2-mediated [3H]TEA transport, respectively, of 4,538 and 1,395 µM for tetramethylammonium, 88.5 and 3.9 µM for tetrapropylammonium, 13.9 and 5.3 µM for tetrabutylammonium, and 8.8 and 7.6 µM for tetrapentylammonium. However, the transporters had very different affinities for cimetidine (CIM): IC50 of 916 and 5.7 µM for rbOCT1 and rbOCT2, respectively. CIM inhibition of TEA uptake into single S2 segments of rabbit proximal tubule was used to estimate the contributions of OCT1 and OCT2 to basolateral organic cation uptake. The median IC50 for CIM inhibition of TEA uptake was 12.3 µM, suggesting that OCT2 is the major contributor to basolateral organic cation transport in the S2 segment of proximal tubule in rabbit kidney.

kidney; proximal tubule; uptake; tetraethylammonium; rabbit organic cation transporter


This article has been cited by other articles:


Home page
 J. Pharmacol. Exp. Ther.Home page
R. M. Pelis, R. C. Hartman, S. H. Wright, T. M. Wunz, and C. E. Groves
Influence of Estrogen and Xenoestrogens on Basolateral Uptake of Tetraethylammonium by Opossum Kidney Cells in Culture
J. Pharmacol. Exp. Ther., November 1, 2007; 323(2): 555 - 561.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. Soodvilai, A. Chatsudthipong, and V. Chatsudthipong
Role of MAPK and PKA in regulation of rbOCT2-mediated renal organic cation transport
Am J Physiol Renal Physiol, July 1, 2007; 293(1): F21 - F27.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
X. Zhang, N. J. Cherrington, and S. H. Wright
Molecular identification and functional characterization of rabbit MATE1 and MATE2-K
Am J Physiol Renal Physiol, July 1, 2007; 293(1): F360 - F370.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. M. Pelis, W. M. Suhre, and S. H. Wright
Functional influence of N-glycosylation in OCT2-mediated tetraethylammonium transport
Am J Physiol Renal Physiol, May 1, 2006; 290(5): F1118 - F1126.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. R. Rheault, D. M. Debicki, and M. J. O'Donnell
Characterization of tetraethylammonium uptake across the basolateral membrane of the Drosophila Malpighian (renal) tubule
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2005; 289(2): R495 - R504.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
X. Zhang, C. E. Groves, A. Bahn, W. M. Barendt, M. D. Prado, M. Rodiger, V. Chatsudthipong, G. Burckhardt, and S. H. Wright
Relative contribution of OAT and OCT transporters to organic electrolyte transport in rabbit proximal tubule
Am J Physiol Renal Physiol, November 1, 2004; 287(5): F999 - F1010.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. H. Wright, K. K. Evans, X. Zhang, N. J. Cherrington, D. S. Sitar, and W. H. Dantzler
Functional map of TEA transport activity in isolated rabbit renal proximal tubules
Am J Physiol Renal Physiol, September 1, 2004; 287(3): F442 - F451.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
J. W. Jonker and A. H. Schinkel
Pharmacological and Physiological Functions of the Polyspecific Organic Cation Transporters: OCT1, 2, and 3 (SLC22A1-3)
J. Pharmacol. Exp. Ther., January 1, 2004; 308(1): 2 - 9.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. Kaewmokul, V. Chatsudthipong, K. K. Evans, W. H. Dantzler, and S. H. Wright
Functional mapping of rbOCT1 and rbOCT2 activity in the S2 segment of rabbit proximal tubule
Am J Physiol Renal Physiol, December 1, 2003; 285(6): F1149 - F1159.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online