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Am J Physiol Renal Physiol 283: F73-F85, 2002. First published January 29, 2002; doi:10.1152/ajprenal.00340.2001
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Vol. 283, Issue 1, F73-F85, July 2002

Expression and function of sodium transporters in two opossum kidney cell clonal sublines

Pedro Gomes1, Jing Xu2, Paula Serrão1, Sofia Dória3, Pedro A. Jose2, and Patrício Soares-da-Silva1

1 Institute of Pharmacology and Therapeutics and 3 Department of Genetics, Faculty of Medicine, 4200-319 Porto, Portugal; and 2 Department of Pediatrics and Department of Physiology and Biophysics, Georgetown University Medical Center, Washington, District of Columbia 20007

The present study describes characteristic features of two clonal subpopulations of opossum kidney (OK) cells (OKLC and OKHC) that are functionally different but morphologically identical. The most impressive differences between OKHC and OKLC cells are the overexpression of Na+-K+-ATPase and type 3 Na+/H+ exchanger by the former, accompanied by an increased Na+-K+-ATPase activity (57.6 ± 5.6 vs. 30.0 ± 0.1 nmol Pi · mg protein-1 · min-1); the increased ability to translocate Na+ from the apical to the basolateral surface; and the increased Na+-dependent pHi recovery (0.254 ± 0.016 vs. 0.094 ± 0.011 pH units/s). Vmax values (in pH units/s) for Na+-dependent pHi recovery in OKHC cells (0.00521 ± 0.0004) were twice (P < 0.05) those in OKLC (0.00202 ± 0.0001), with similar Km values (in mM) for Na+ (OKLC, 21.0 ± 5.5; OKHC, 14.0 ± 5.6). In addition, we measured the activities of transporters (organic ions, alpha -methyl-D-glucoside, L-type amino acids, and Na+) and enzymes (adenylyl cyclase, aromatic L-amino acid decarboxylase, and catechol-O-methyltransferase). The cells were also characterized morphologically by optical and scanning electron microscopy and karyotyped. It is suggested that OKLC and OKHC cells constitute an interesting cell model for the study of renal epithelial physiology and pathophysiology, namely, hypertension.

sodium-hydrogen exchanger; sodium-potassium adenosine 5'-triphosphatase; hypertension


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