| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of 1 Medicine and 3 Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas 75390-8856; 2 Department of Biomedical Science, University of Sheffield, Sheffield S102TN, United Kingdom; and 4 Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland 21201
ROMK channels are present in the cortical
collecting ducts of kidney and are responsible for K+
secretion in this nephron segment. Recent studies suggest that endocytosis of ROMK channels is important for regulation of
K+ secretion in cortical collecting ducts. We investigated
the molecular mechanisms for endocytosis of ROMK channels expressed in
Xenopus laevis oocytes and cultured Madin-Darby canine
kidney cells. When plasma membrane insertion of newly synthesized
channel proteins was blocked by incubation with brefeldin A, ROMK
currents decreased with a half-time of ~6 h. Coexpression with the
Lys44
Ala dominant-negative mutant dynamin, but not wild-type
dynamin, reduced the rate of reduction of ROMK in the presence of
brefeldin A. Mutation of Asn371 to Ile in the putative NPXY
internalization motif of ROMK1 abolished the effect of the Lys44Ala
dynamin mutant on endocytosis of the channel. Coimmunoprecipitation
study and confocal fluorescent imaging revealed that ROMK channels
associated with clathrin coat proteins in Madin-Darby canine kidney
cells. These results provide compelling evidence for endocytosis of
ROMK channels via clathrin-coated vesicles.
dominant-negative dynamin; Madin-Darby canine kidney cells; brefeldin A; Xenopus laevis oocytes; tyrosine-based consensus motif
This article has been cited by other articles:
|
|
 |
|
  H. Hibino, A. Inanobe, K. Furutani, S. Murakami, I. Findlay, and Y. Kurachi Inwardly Rectifying Potassium Channels: Their Structure, Function, and Physiological Roles Physiol Rev, January 1, 2010; 90(1): 291 - 366. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Perry, O. J. Baker, M. E. Reyland, and I. I. Grichtchenko PKC{alpha}{beta}{gamma}- and PKC{delta}-dependent endocytosis of NBCe1-A and NBCe1-B in salivary parotid acinar cells Am J Physiol Cell Physiol, December 1, 2009; 297(6): C1409 - C1423. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.-H. Lin, P. Yue, C.-Y. Pan, P. Sun, X. Zhang, Z. Han, M. Roos, M. Caplan, G. Giebisch, and W.-H. Wang POSH Stimulates the Ubiquitination and the Clathrin-independent Endocytosis of ROMK1 Channels J. Biol. Chem., October 23, 2009; 284(43): 29614 - 29624. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Welling and K. Ho A comprehensive guide to the ROMK potassium channel: form and function in health and disease Am J Physiol Renal Physiol, October 1, 2009; 297(4): F849 - F863. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-K. Cha, M.-C. Hu, H. Kurosu, M. Kuro-o, O. Moe, and C.-L. Huang Regulation of Renal Outer Medullary Potassium Channel and Renal K+ Excretion by Klotho Mol. Pharmacol., July 1, 2009; 76(1): 38 - 46. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mertl, H. Daniel, and G. Kottra Substrate-induced changes in the density of peptide transporter PEPT1 expressed in Xenopus oocytes Am J Physiol Cell Physiol, November 1, 2008; 295(5): C1332 - C1343. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-R. Wang, Z. Liu, and C.-L. Huang Domains of WNK1 kinase in the regulation of ROMK1 Am J Physiol Renal Physiol, August 1, 2008; 295(2): F438 - F445. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-B. Peng and D. G. Warnock WNK4-mediated regulation of renal ion transport proteins Am J Physiol Renal Physiol, October 1, 2007; 293(4): F961 - F973. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Perry, H. Le, and I. I. Grichtchenko ANG II and calmodulin/CaMKII regulate surface expression and functional activity of NBCe1 via separate means Am J Physiol Renal Physiol, July 1, 2007; 293(1): F68 - F77. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Golbang, G. Cope, A. Hamad, M. Murthy, C.-H. Liu, A. W. Cuthbert, and K. M. O'Shaughnessy Regulation of the expression of the Na/Cl cotransporter by WNK4 and WNK1: evidence that accelerated dynamin-dependent endocytosis is not involved Am J Physiol Renal Physiol, December 1, 2006; 291(6): F1369 - F1376. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-H. Wang Regulation of ROMK (Kir1.1) channels: new mechanisms and aspects Am J Physiol Renal Physiol, January 1, 2006; 290(1): F14 - F19. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-J. Sun, W.-Z. Zeng, and C.-L. Huang Inhibition of ROMK potassium channel by syntaxin 1A Am J Physiol Renal Physiol, February 1, 2005; 288(2): F284 - F289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Hebert, G. Desir, G. Giebisch, and W. Wang Molecular Diversity and Regulation of Renal Potassium Channels Physiol Rev, January 1, 2005; 85(1): 319 - 371. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Nesti, B. Everill, and A. D. Morielli Endocytosis as a Mechanism for Tyrosine Kinase-dependent Suppression of a Voltage-gated Potassium Channel Mol. Biol. Cell, September 1, 2004; 15(9): 4073 - 4088. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-Y. Chu, R. Quigley, V. Babich, and C.-L. Huang Dietary potassium restriction stimulates endocytosis of ROMK channel in rat cortical collecting duct Am J Physiol Renal Physiol, December 1, 2003; 285(6): F1179 - F1187. [Abstract] [Full Text]
|
|
|
|
|
|
C. A. Bertrand and R. A. Frizzell The role of regulated CFTR trafficking in epithelial secretion Am J Physiol Cell Physiol, July 1, 2003; 285(1): C1 - C18. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
