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1 Department of Medicine and 5 Division of Nephrology, Hypertension and Transplantation, University of Florida, Gainesville, Florida 32610; 3 Department of Physiology, School of Medicine, Dongguk University, Kyungju, 780-714; 4 Department of Anatomy, College of Medicine, Catholic University, Seoul 137-701, Korea; and 2 The Water and Salt Research Center, University of Aarhus, DK-8000 Aarhus C, Denmark
Recent studies have demonstrated that a
novel anion exchanger, pendrin, is expressed in the apical domain of
type B intercalated cells in the mammalian collecting duct. The purpose
of this study was 1) to determine the expression and
distribution of pendrin along the collecting duct and connecting tubule
of mouse and rat kidney and establish whether pendrin is expressed in
the non-A-non-B intercalated cells and 2) to determine the
intracellular localization of pendrin in the different populations of
intercalated cells by immunoelectron microscopy. A peptide-derived
affinity-purified antibody was generated that specifically recognized
pendrin in immunoblots of rat and mouse kidney. Immunohistochemistry
and confocal laser scanning microscopy demonstrated the presence of pendrin in apical domains of all type B intercalated cells in mouse and
rat connecting tubule and collecting duct. In addition, strong pendrin
immunostaining was observed in non-A-non-B intercalated cells. There
was no labeling of type A intercalated cells. Immunoelectron microscopy
demonstrated that pendrin was located in the apical plasma membrane and
intracellular vesicles of both type B intercalated cells and
non-A-non-B cells; the latter was identified by the presence of
H+-ATPase in the apical plasma membrane. The results of
this study demonstrate that both pendrin and H+-ATPase are
expressed in the apical plasma membrane of non-A-non-B intercalated
cells, suggesting that these cells are capable of both
HCO
acid-base metabolism; connecting tubule; collecting duct; bicarbonate secretion
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