AJP - Renal Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 283: F923-F933, 2002. First published June 26, 2002; doi:10.1152/ajprenal.00015.2002
0363-6127/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/5/F923    most recent
00015.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (19)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nielsen, J.
Right arrow Articles by Knepper, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nielsen, J.
Right arrow Articles by Knepper, M. A.
Vol. 283, Issue 5, F923-F933, November 2002

Sodium transporter abundance profiling in kidney: effect of spironolactone

Jakob Nielsen1,2, Tae-Hwan Kwon2, Shyama Masilamani1, Kathleen Beutler1, Henrik Hager2, Søren Nielsen2, and Mark A. Knepper1

1 Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892-1603; and 2 The Water and Salt Research Center, University of Aarhus, DK-8000 Aarhus C, Denmark

Renal tubule profiling studies were carried out to investigate the long-term effects of administration of spironolactone, a mineralocorticoid receptor antagonist, on abundances of the major Na transporter and Na channel proteins along the rat renal tubule. Oral administration of spironolactone for 7 days to NaCl-restricted rats did not significantly alter abundances of Na transporters expressed proximal to the macula densa, while substantially decreasing the abundances of the thiazide-sensitive Na-Cl cotransporter (NCC), the alpha -subunit of the amiloride-sensitive epithelial Na channel (ENaC), and the 70-kDa form of the gamma -subunit of ENaC. A dependency of NCC expression on aldosterone was confirmed by showing increased NCC expression in response to aldosterone infusion in adrenalectomized rats. Immunoperoxidase labeling of ENaC in renal cortex confirmed that dietary NaCl restriction causes a redistribution of ENaC to the apical domain of connecting tubule cells and showed that high-dose spironolactone administration does not block this apical redistribution. In contrast, spironolactone completely blocked the increase in alpha -ENaC abundance in response to dietary NaCl restriction. We conclude that the protein abundances of NCC, alpha -ENaC, and the 70-kDa form of gamma -ENaC are regulated via the classical mineralocorticoid receptor, but the subcellular redistribution of ENaC in response to dietary NaCl restriction is not prevented by blockade of the mineralocorticoid receptor.

aldosterone; collecting duct; distal convoluted tubule


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
M. B. Sandberg, A. D. M. Riquier, K. Pihakaski-Maunsbach, A. A. McDonough, and A. B. Maunsbach
ANG II provokes acute trafficking of distal tubule Na+-Cl cotransporter to apical membrane
Am J Physiol Renal Physiol, September 1, 2007; 293(3): F662 - F669.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. M. Ortiz, M. L. Graciano, D. Seth, M. S. Awayda, and L. G. Navar
Aldosterone receptor antagonism exacerbates intrarenal angiotensin II augmentation in ANG II-dependent hypertension
Am J Physiol Renal Physiol, July 1, 2007; 293(1): F139 - F147.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
C. Ronzaud, J. Loffing, M. Bleich, N. Gretz, H.-J. Grone, G. Schutz, and S. Berger
Impairment of Sodium Balance in Mice Deficient in Renal Principal Cell Mineralocorticoid Receptor
J. Am. Soc. Nephrol., June 1, 2007; 18(6): 1679 - 1687.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Nielsen, T.-H. Kwon, J. Frokiaer, M. A. Knepper, and S. Nielsen
Maintained ENaC trafficking in aldosterone-infused rats during mineralocorticoid and glucocorticoid receptor blockade
Am J Physiol Renal Physiol, January 1, 2007; 292(1): F382 - F394.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. D. Klein, B. P. Murrell, S. Tucker, Y.-H. Kim, and J. M. Sands
Urea transporter UT-A1 and aquaporin-2 proteins decrease in response to angiotensin II or norepinephrine-induced acute hypertension
Am J Physiol Renal Physiol, November 1, 2006; 291(5): F952 - F959.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. B. Sandberg, A. B. Maunsbach, and A. A. McDonough
Redistribution of distal tubule Na+-Cl- cotransporter (NCC) in response to a high-salt diet
Am J Physiol Renal Physiol, August 1, 2006; 291(2): F503 - F508.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. de Seigneux, S. W. Kim, S. C. Hemmingsen, J. Frokiaer, and S. Nielsen
Increased expression but not targeting of ENaC in adrenalectomized rats with PAN-induced nephrotic syndrome
Am J Physiol Renal Physiol, July 1, 2006; 291(1): F208 - F217.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Nielsen, T.-H. Kwon, J. Frokiaer, M. A. Knepper, and S. Nielsen
Lithium-induced NDI in rats is associated with loss of {alpha}-ENaC regulation by aldosterone in CCD
Am J Physiol Renal Physiol, May 1, 2006; 290(5): F1222 - F1233.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. W. Kim, S. de Seigneux, M. C. Sassen, J. Lee, J. Kim, M. A. Knepper, J. Frokiaer, and S. Nielsen
Increased apical targeting of renal ENaC subunits and decreased expression of 11betaHSD2 in HgCl2-induced nephrotic syndrome in rats
Am J Physiol Renal Physiol, March 1, 2006; 290(3): F674 - F687.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
G. Gamba
Molecular Physiology and Pathophysiology of Electroneutral Cation-Chloride Cotransporters
Physiol Rev, April 1, 2005; 85(2): 423 - 493.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. Bachmann, K. Mutig, J. Bates, P. Welker, B. Geist, V. Gross, F. C. Luft, N. Alenina, M. Bader, B. J. Thiele, et al.
Renal effects of Tamm-Horsfall protein (uromodulin) deficiency in mice
Am J Physiol Renal Physiol, March 1, 2005; 288(3): F559 - F567.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
V. Vallon, P. Wulff, D. Y. Huang, J. Loffing, H. Volkl, D. Kuhl, and F. Lang
Role of Sgk1 in salt and potassium homeostasis
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2005; 288(1): R4 - R10.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. Sepehrdad, P. N. Chander, G. Singh, and C. T. Stier Jr
Sodium transport antagonism reduces thrombotic microangiopathy in stroke-prone spontaneously hypertensive rats
Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1185 - F1192.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
B. W. M. van Balkom, J. D. Hoffert, C.-L. Chou, and M. A. Knepper
Proteomic analysis of long-term vasopressin action in the inner medullary collecting duct of the Brattleboro rat
Am J Physiol Renal Physiol, February 1, 2004; 286(2): F216 - F224.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Nielsen, T.-H. Kwon, J. Praetorius, Y.-H. Kim, J. Frokiaer, M. A. Knepper, and S. Nielsen
Segment-specific ENaC downregulation in kidney of rats with lithium-induced NDI
Am J Physiol Renal Physiol, December 1, 2003; 285(6): F1198 - F1209.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Renal Physiol.Home page
O. A. Weisz and J. P. Johnson
Noncoordinate regulation of ENaC: paradigm lost?
Am J Physiol Renal Physiol, November 1, 2003; 285(5): F833 - F842.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. Turban, X.-Y. Wang, and M. A. Knepper
Regulation of NHE3, NKCC2, and NCC abundance in kidney during aldosterone escape phenomenon: role of NO
Am J Physiol Renal Physiol, November 1, 2003; 285(5): F843 - F851.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
T.-H. Kwon, J. Nielsen, Y.-H. Kim, M. A. Knepper, J. Frokiaer, and S. Nielsen
Regulation of sodium transporters in the thick ascending limb of rat kidney: response to angiotensin II
Am J Physiol Renal Physiol, July 1, 2003; 285(1): F152 - F165.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
K. T. Beutler, S. Masilamani, S. Turban, J. Nielsen, H. L. Brooks, S. Ageloff, R. A. Fenton, R. K. Packer, and M. A. Knepper
Long-Term Regulation of ENaC Expression in Kidney by Angiotensin II
Hypertension, May 1, 2003; 41(5): 1143 - 1150.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Loffing and B. Kaissling
Sodium and calcium transport pathways along the mammalian distal nephron: from rabbit to human
Am J Physiol Renal Physiol, April 1, 2003; 284(4): F628 - F643.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online