AJP - Renal Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 283: F1351-F1364, 2002. First published August 6, 2002; doi:10.1152/ajprenal.0224.2001
0363-6127/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow A corrigendum has been published
Right arrow All Versions of this Article:
283/6/F1351    most recent
0224.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (8)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zharkikh, L.
Right arrow Articles by Nelson, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zharkikh, L.
Right arrow Articles by Nelson, R. D.
Vol. 283, Issue 6, F1351-F1364, December 2002

Renal principal cell-specific expression of green fluorescent protein in transgenic mice

Ludmilla Zharkikh1, Xiaohong Zhu1, Peter K. Stricklett2, Donald E. Kohan2, Greg Chipman1, Sylvie Breton3,4, Dennis Brown3, and Raoul D. Nelson1

Departments of 1 Pediatrics and 2 Medicine, University of Utah, Salt Lake City, Utah 84132; and 3 Program in Membrane Biology and Renal Unit, Massachusetts General Hospital, and 4 Department of Medicine, Harvard Medical School, Boston, Massachusetts 02114

The purpose of this study is to develop transgenic mice with principal cell-specific expression of green fluorescent protein (GFP). After the cloning and sequencing of the mouse aquaporin-2 (AQP2) gene, 9.5 kb of the promoter were used to drive expression of GFP in transgenic mice. In transgenic mice, GFP was selectively expressed in principal cells of the renal collecting duct and not in intercalated cells. Expression was increased by dehydration of mice. AQP2 and GFP expression was maintained in primary cultures of renal medulla that were stimulated with cAMP or vasopressin analogs. GFP-expressing cells were then isolated by fluorescence-activated cell sorting. RT-PCR analysis showed expression of AQP2, AQP3, AQP4, vasopressin type 2 receptor, and cAMP response element binding protein but not H+-ATPase B1 subunit or anion exchanger 1. After expansion of these cells in culture, RT-PCR analysis showed continued expression of the same genes. This pattern of gene expression is that of principal cells rather than intercalated cells. This transgenic mouse model can be used in future studies of gene expression during the development, differentiation, and maturation of renal principal cells.

renal collecting duct; aquaporin-2; gene expression regulation


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
M.-J. Yu, R. L. Miller, P. Uawithya, M. M. Rinschen, S. Khositseth, D. W. W. Braucht, C.-L. Chou, T. Pisitkun, R. D. Nelson, and M. A. Knepper
Systems-level analysis of cell-specific AQP2 gene expression in renal collecting duct
PNAS, February 17, 2009; 106(7): 2441 - 2446.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
C. Ronzaud, J. Loffing, M. Bleich, N. Gretz, H.-J. Grone, G. Schutz, and S. Berger
Impairment of Sodium Balance in Mice Deficient in Renal Principal Cell Mineralocorticoid Receptor
J. Am. Soc. Nephrol., June 1, 2007; 18(6): 1679 - 1687.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. L. Miller, P. Zhang, T. Chen, A. Rohrwasser, and R. D. Nelson
Automated method for the isolation of collecting ducts
Am J Physiol Renal Physiol, July 1, 2006; 291(1): F236 - F245.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
W. Ye, H. Zhang, E. Hillas, D. E. Kohan, R. L. Miller, R. D. Nelson, M. Honeggar, and T. Yang
Expression and function of COX isoforms in renal medulla: evidence for regulation of salt sensitivity and blood pressure
Am J Physiol Renal Physiol, February 1, 2006; 290(2): F542 - F549.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. A. Fenton, A. Shodeinde, and M. A. Knepper
UT-A urea transporter promoter, UT-A{alpha}, targets principal cells of the renal inner medullary collecting duct
Am J Physiol Renal Physiol, January 1, 2006; 290(1): F188 - F195.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. L. Miller, P. Zhang, M. Smith, V. Beaulieu, T. G. Paunescu, D. Brown, S. Breton, and R. D. Nelson
V-ATPase B1-subunit promoter drives expression of EGFP in intercalated cells of kidney, clear cells of epididymis and airway cells of lung in transgenic mice
Am J Physiol Cell Physiol, May 1, 2005; 288(5): C1134 - C1144.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
E. I. Veizis, C. R. Carlin, and C. U. Cotton
Decreased amiloride-sensitive Na+ absorption in collecting duct principal cells isolated from BPK ARPKD mice
Am J Physiol Renal Physiol, February 1, 2004; 286(2): F244 - F254.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online