AJP - Renal Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 284: F133-F143, 2003. First published September 24, 2002; doi:10.1152/ajprenal.00227.2002
0363-6127/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/1/F133    most recent
00227.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (39)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Na, K. Y.
Right arrow Articles by Kim, G.-H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Na, K. Y.
Right arrow Articles by Kim, G.-H.
Vol. 284, Issue 1, F133-F143, January 2003

Upregulation of Na+ transporter abundances in response to chronic thiazide or loop diuretic treatment in rats

Ki Young Na1, Yoon Kyu Oh2, Jin Suk Han1, Kwon Wook Joo1, Jung Sang Lee1, Jae-Ho Earm3, Mark A. Knepper4, and Gheun-Ho Kim5

1 Department of Internal Medicine, Seoul National University, Clinical Research Institute of Seoul National University Hospital, 2 Department of Internal Medicine, Eulji Medical College, 5 Department of Internal Medicine, Hallym University Hangang Sacred Heart Hospital, Seoul, 110-744; 3 Department of Internal Medicine, Chungbuk National University, Cheongju 361-711, South Korea; 4  and Laboratory of Kidney and Electrolyte Metabolism, National Institutes of Health, Bethesda, Maryland 20892

Furosemide and hydrochlorothiazide (HCTZ) exert their diuretic actions by binding to apical Na+ transporters, viz., the Na+-K+-2Cl- cotransporter in the thick ascending limb and the Na+-Cl- cotransporter in the distal convoluted tubule, respectively. We carried out semiquantitative immunoblotting and immunohistochemistry of rat kidneys to investigate whether chronic administration of furosemide or HCTZ is associated with compensatory changes in the abundance of Na+ transporters downstream from the primary site of action. Osmotic minipumps were implanted into Sprague-Dawley rats to deliver furosemide (12 mg/day) or HCTZ (3.75 mg/day) for 7 days. To prevent volume depletion, all animals were offered tap water and a solution containing 0.8% NaCl and 0.1% KCl as drinking fluid. The diuretic/natriuretic response was quantified in response to both agents by using quantitative urine collections. Semiquantitative immunoblotting revealed that the abundances of thick ascending limb Na+-K+-2Cl- cotransporter and all three subunits of the epithelial Na+ channel (ENaC) were increased by furosemide infusion. HCTZ infusion increased the abundances of thiazide-sensitive Na+-Cl- cotransporter and beta -ENaC in the cortex and beta - and gamma -ENaC in the outer medulla. Consistent with these results, beta -ENaC immunohistochemistry showed a remarkable increase in immunoreactivity in the principal cells of collecting ducts with either diuretic treatment. These increases in the abundance of Na+ transporters in response to chronic diuretic treatment may account for the generation of diuretic tolerance associated with long-term diuretic use.

collecting duct; epithelial sodium channel; distal convoluted tubule; thiazide-sensitive sodium-chloride cotransporter


This article has been cited by other articles:


Home page
 HypertensionHome page
F. Luo, Y. Wang, X. Wang, K. Sun, X. Zhou, and R. Hui
A Functional Variant of NEDD4L Is Associated With Hypertension, Antihypertensive Response, and Orthostatic Hypotension
Hypertension, October 1, 2009; 54(4): 796 - 801.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. A. Wagner, D. Loffing-Cueni, Q. Yan, N. Schulz, P. Fakitsas, M. Carrel, T. Wang, F. Verrey, J. P. Geibel, G. Giebisch, et al.
Mouse model of type II Bartter's syndrome. II. Altered expression of renal sodium- and water-transporting proteins
Am J Physiol Renal Physiol, June 1, 2008; 294(6): F1373 - F1380.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
V. Pech, W. Zheng, T. D. Pham, J. W. Verlander, and S. M. Wall
Angiotensin II Activates H+-ATPase in Type A Intercalated Cells
J. Am. Soc. Nephrol., January 1, 2008; 19(1): 84 - 91.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. E. Yang, P. K. K. Leong, and A. A. McDonough
Reducing blood pressure in SHR with enalapril provokes redistribution of NHE3, NaPi2, and NCC and decreases NaPi2 and ACE abundance
Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1197 - F1208.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Y. H. Kim, V. Pech, K. B. Spencer, W. H. Beierwaltes, L. A. Everett, E. D. Green, W. Shin, J. W. Verlander, R. L. Sutliff, and S. M. Wall
Reduced ENaC protein abundance contributes to the lower blood pressure observed in pendrin-null mice
Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1314 - F1324.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. Andrade, A. C. Rodrigues Jr., T. R. C. Sanches, R. B. Souza, and A. C. Seguro
Leptospirosis leads to dysregulation of sodium transporters in the kidney and lung
Am J Physiol Renal Physiol, February 1, 2007; 292(2): F586 - F592.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Song, X. Hu, S. Riazi, S. Tiwari, J. B. Wade, and C. A. Ecelbarger
Regulation of blood pressure, the epithelial sodium channel (ENaC), and other key renal sodium transporters by chronic insulin infusion in rats
Am J Physiol Renal Physiol, May 1, 2006; 290(5): F1055 - F1064.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
G. Gamba
Molecular Physiology and Pathophysiology of Electroneutral Cation-Chloride Cotransporters
Physiol Rev, April 1, 2005; 85(2): 423 - 493.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
N. Jeck, K. P. Schlingmann, S. C. Reinalter, M. Komhoff, M. Peters, S. Waldegger, and H. W. Seyberth
Salt handling in the distal nephron: lessons learned from inherited human disorders
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2005; 288(4): R782 - R795.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
Z. Zhu, S. Zhu, D. Liu, T. Cao, L. Wang, and M. Tepel
Thiazide-Like Diuretics Attenuate Agonist-Induced Vasoconstriction by Calcium Desensitization Linked to Rho Kinase
Hypertension, February 1, 2005; 45(2): 233 - 239.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
P. A. Sonalker, S. P. Tofovic, and E. K. Jackson
Increased Expression of the Sodium Transporter BSC-1 in Spontaneously Hypertensive Rats
J. Pharmacol. Exp. Ther., December 1, 2004; 311(3): 1052 - 1061.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C.-T. Lee, S. Shang, L.-W. Lai, K.-C. Yong, and Y.-H. H. Lien
Effect of thiazide on renal gene expression of apical calcium channels and calbindins
Am J Physiol Renal Physiol, December 1, 2004; 287(6): F1164 - F1170.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
G.-H. Kim, J. W. Lee, Y. K. Oh, H. R. Chang, K. W. Joo, K. Y. Na, J.-H. Earm, M. A. Knepper, and J. S. Han
Antidiuretic Effect of Hydrochlorothiazide in Lithium-Induced Nephrogenic Diabetes Insipidus Is Associated with Upregulation of Aquaporin-2, Na-Cl Co-transporter, and Epithelial Sodium Channel
J. Am. Soc. Nephrol., November 1, 2004; 15(11): 2836 - 2843.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
A. Aviv, N. K. Hollenberg, and A. Weder
Urinary Potassium Excretion and Sodium Sensitivity in Blacks
Hypertension, April 1, 2004; 43(4): 707 - 713.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
T. Nijenhuis, J. G.J. Hoenderop, and R. J.M. Bindels
Downregulation of Ca2+ and Mg2+ Transport Proteins in the Kidney Explains Tacrolimus (FK506)-Induced Hypercalciuria and Hypomagnesemia
J. Am. Soc. Nephrol., March 1, 2004; 15(3): 549 - 557.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Song, M. A. Knepper, J. G. Verbalis, and C. A. Ecelbarger
Increased renal ENaC subunit and sodium transporter abundances in streptozotocin-induced type 1 diabetes
Am J Physiol Renal Physiol, December 1, 2003; 285(6): F1125 - F1137.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Renal Physiol.Home page
O. A. Weisz and J. P. Johnson
Noncoordinate regulation of ENaC: paradigm lost?
Am J Physiol Renal Physiol, November 1, 2003; 285(5): F833 - F842.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Lebowitz, B. An, R. S. Edinger, M. L. Zeidel, and J. P. Johnson
Effect of altered Na+ entry on expression of apical and basolateral transport proteins in A6 epithelia
Am J Physiol Renal Physiol, September 1, 2003; 285(3): F524 - F531.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online