| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of Pediatrics and Physiology and Biophysics, Rainbow Center for Childhood PKD, Case Western Reserve University, Cleveland, Ohio 44106-4948
The effects of the ERK pathway on
electrogenic transepithelial Na+ absorption by renal
collecting duct cells were determined. Approximately 90% of the
unstimulated short-circuit current (15 ± 1 µA/cm2,
n = 10) across conditionally immortalized murine
collecting duct epithelial cells (mCT1) is amiloride sensitive and is
likely mediated by apical epithelial Na+ channels. Chronic
exposure (24 h) of the epithelial monolayers to either EGF (50 ng/ml)
or transforming growth factor-
(TGF-; 20 ng/ml) reduced
amiloride-sensitive short-circuit current by >60%. The inhibitory
effect of EGF on Na+ absorption was not due to inhibition
of basolateral Na+-K+-ATPase, because the pump
current elicited by permeabilization of apical membrane with nystatin
was not reduced by EGF. Chronic exposure of the mCT1 cells to EGF (20 ng/ml, 24 h) elicited a 70-85% decrease in epithelial
Na+ channel subunit mRNA levels. Exposure of mCT1 cells to
either EGF (20 ng/ml) or PMA (150 nM) induced rapid phosphorylation of p42/p44 (ERK1/2) and pretreatment of the monolayers with PD-98059 (an
ERK kinase inhibitor; 30 µM) prevented phosphorylation of p42/p44. Similarly, pretreatment of mCT1 monolayers with
PD-98059 prevented the EGF- and PMA-induced inhibition of
amiloride-sensitive Na+ absorption. The results of these
studies demonstrate that amiloride-sensitive Na+ absorption
by renal collecting duct cells is regulated by the ERK pathway. This
pathway may play a role in alterations in ion transport that occur in
polycystic kidney disease.
extracellular signal-regulated protein kinase; ERK1/2; polycystic kidney disease; mitogen-activated protein kinase
This article has been cited by other articles:
|
|
 |
|
  L. Liu, B. J. Duke, B. Malik, Q. Yue, and D. C. Eaton Biphasic regulation of ENaC by TGF-{alpha} and EGF in renal epithelial cells Am J Physiol Renal Physiol, June 1, 2009; 296(6): F1417 - F1427. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Vasquez, S. B. Mustafa, A. Choudary, S. R. Seidner, and R. Castro Regulation of Epithelial Na+ Channel (ENaC) in the Salivary Cell Line SMG-C6 Experimental Biology and Medicine, May 1, 2009; 234(5): 522 - 531. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-T. Chang, C.-C. Hung, Y.-C. Chen, T.-H. Yen, M.-S. Wu, C.-W. Yang, A. Phillips, and Y.-C. Tian Transforming growth factor-{beta}1 decreases epithelial sodium channel functionality in renal collecting duct cells via a Smad4-dependent pathway Nephrol. Dial. Transplant., April 1, 2008; 23(4): 1126 - 1134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Falin and C. U. Cotton Acute Downregulation of ENaC by EGF Involves the PY Motif and Putative ERK Phosphorylation Site J. Gen. Physiol., August 27, 2007; 130(3): 313 - 328. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Bhalla, R. Soundararajan, A. C. Pao, H. Li, and D. Pearce Disinhibitory pathways for control of sodium transport: regulation of ENaC by SGK1 and GILZ Am J Physiol Renal Physiol, October 1, 2006; 291(4): F714 - F721. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Olteanu, B. K. Yoder, W. Liu, M. J. Croyle, E. A. Welty, K. Rosborough, J. M. Wyss, P. D. Bell, L. M. Guay-Woodford, M. O. Bevensee, et al. Heightened epithelial Na+ channel-mediated Na+ absorption in a murine polycystic kidney disease model epithelium lacking apical monocilia Am J Physiol Cell Physiol, April 1, 2006; 290(4): C952 - C963. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Markadieu, R. Crutzen, D. Blero, C. Erneux, and R. Beauwens Hydrogen peroxide and epidermal growth factor activate phosphatidylinositol 3-kinase and increase sodium transport in A6 cell monolayers Am J Physiol Renal Physiol, June 1, 2005; 288(6): F1201 - F1212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Falin, I. E. Veizis, and C. U. Cotton A role for ERK1/2 in EGF- and ATP-dependent regulation of amiloride-sensitive sodium absorption Am J Physiol Cell Physiol, May 1, 2005; 288(5): C1003 - C1011. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. E. Veizis and C. U. Cotton Abnormal EGF-dependent regulation of sodium absorption in ARPKD collecting duct cells Am J Physiol Renal Physiol, March 1, 2005; 288(3): F474 - F482. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Tong and J. D. Stockand Receptor tyrosine kinases mediate epithelial Na+ channel inhibition by epidermal growth factor Am J Physiol Renal Physiol, January 1, 2005; 288(1): F150 - F161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Grossmann, R. Freudinger, S. Mildenberger, A. W. Krug, and M. Gekle Evidence for epidermal growth factor receptor as negative-feedback control in aldosterone-induced Na+ reabsorption Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1226 - F1231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. I. Veizis, C. R. Carlin, and C. U. Cotton Decreased amiloride-sensitive Na+ absorption in collecting duct principal cells isolated from BPK ARPKD mice Am J Physiol Renal Physiol, February 1, 2004; 286(2): F244 - F254. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
