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Am J Physiol Renal Physiol 284: F411-F417, 2003. First published October 22, 2002; doi:10.1152/ajprenal.00235.2002
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Vol. 284, Issue 2, F411-F417, February 2003

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Differential, inducible gene targeting in renal epithelia, vascular endothelium, and viscera of Mx1Cre mice

André Schneider1, Yahua Zhang2, Youfei Guan2, Linda S. Davis2, and Matthew D. Breyer2,3

1 Universitätsklinikum Eppendorf, 20246 Hamburg, Germany; 2 Division of Nephrology, Department of Medicine, Veterans Affairs Medical Center, and 3 Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee 37212-2372

The Cre/loxP transgenic system may be used to achieve temporally and/or spatially regulated gene deletion. The Mx1Cre mouse expresses Cre recombinase under control of the IFN-inducible Mx1 promoter. Mx1Cre mice were crossed with a reporter strain (ROSA26tm1Sor) in which beta -galactosidase activity is expressed only after Cre-mediated recombination to determine the cellular pattern of Cre-mediated genetic recombination in the kidney and other tissues. Widespread recombination was observed in vascular endothelium as well as in the liver and spleen. Recombination was restricted to subsets of stromal cells in uterus, duodenum, colon, aorta, and kidney. In the cortex, chi -galactosidase activity was detected in a subset of tubules and all glomerular cells, including endothelium, mesangium, and podocytes. No chi -galactosidase activity was detected in proximal tubules. Costaining of kidneys with segment-specific markers demonstrated induction of chi -galactosidase activity in collecting duct, with sporadic labeling of the thick ascending limb but no significant labeling of distal convoluted tubules. We conclude that Mx1-driven gene recombination is spatially as well as temporally restricted. The Mx1Cre transgene should prove a useful reagent to achieve temporally regulated recombination in endothelial, glomerular, and distal renal epithelia in mice.

Cre recombinase; liver; spleen; interstitium; collecting duct; glomerulus


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