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Am J Physiol Renal Physiol 284: F644-F652, 2003. First published December 27, 2002; doi:10.1152/ajprenal.00274.2002
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Vol. 284, Issue 4, F644-F652, April 2003

Effect of partial outlet obstruction on rabbit urinary bladder smooth muscle function

Xiaoling Su1, Raymund Stein2, Michaela C. Stanton1, Stephen Zderic2, and Robert S. Moreland1

1 Department of Pharmacology and Physiology, Drexel University College of Medicine, Philadelphia 19102; and 2 Department of Urology, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19101

Bladder outlet obstruction secondary to benign prostate hyperplasia is associated with many cellular changes. This study was designed to determine whether these changes involve the contractile apparatus. Bladder smooth muscles from rabbits subjected to partial outlet obstruction for 2 wk were mounted for isometric force, isotonic shortening velocity, and myosin light chain (MLC) phosphorylation levels. Muscle strips from obstructed bladders exhibited spontaneous phasic activity; muscle strips from control bladders did not. Muscle strips from obstructed bladders exhibited increased sensitivity and higher levels of stress in response to the cumulative addition of KCl or carbachol compared with control. During noncumulative addition of KCl or carbachol, no differences in sensitivity were noted. Muscle strips from obstructed bladders had elevated basal MLC phosphorylation levels and stimulation produced small increases in MLC phosphorylation compared with control. Vmax during KCl stimulation of muscle strips from obstructed bladders was 10-fold lower than control. Our results suggest that bladder outlet obstruction produces a muscle cell that develops higher levels of force but with greatly reduced cross bridge cycling rates.

benign prostatic hyperplasia; shortening velocity; myosin light chain phosphorylation; carbachol


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