AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 284: F663-F670, 2003. First published December 10, 2002; doi:10.1152/ajprenal.00332.2002
0363-6127/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/4/F663    most recent
00332.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (7)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Moz, Y.
Right arrow Articles by Naveh-Many, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Moz, Y.
Right arrow Articles by Naveh-Many, T.
Vol. 284, Issue 4, F663-F670, April 2003

Characterization of cis-acting element in renal NaPi-2 cotransporter mRNA that determines mRNA stability

Yulia Moz, Justin Silver, and Tally Naveh-Many

Minerva Center for Calcium and Bone Metabolism, Nephrology Services, Hadassah University Hospital, Jerusalem, Israel 91120

Hypophosphatemia leads to an increase in Na+-Pi cotransporter (NaPi-2) mRNA levels. This increase is posttranscriptional and correlates with a more stable transcript mediated by the terminal 698 nt of the NaPi-2 mRNA. A 71-nt binding element was identified with renal proteins from rats fed control and low-Pi (-Pi) diet. The binding of -Pi renal proteins to this transcript was increased compared with control proteins. The functionality of the cis element was demonstrated by an in vitro degradation assay. -Pi renal proteins stabilized transcripts that included the cis element compared with control renal extracts. The full-length NaPi-2 transcript, but not control transcripts, was stabilized by -Pi extracts. Insertion of the binding element into green fluorescent protein (GFP) as a reporter gene decreased chimeric GFP mRNA levels in transfection experiments. Our results suggest that the protein-binding region of the NaPi-2 mRNA functions as a cis-acting instability element. In hypophosphatemia there is increased binding to the cis-acting element and subsequent stabilization of NaPi-2 mRNA.

phosphate; messenger ribonucleic acid half-life; protein-ribonucleic acid interactions


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
S. J. Khundmiri, A. Ahmad, R. E. Bennett, E. J. Weinman, D. Steplock, J. Cole, P. D. Baumann, J. Lewis, S. Singh, B. J. Clark, et al.
Novel regulatory function for NHERF-1 in Npt2a transcription
Am J Physiol Renal Physiol, April 1, 2008; 294(4): F840 - F849.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
Y. Moz, R. Levi, V. Lavi-Moshayoff, K. B. Cox, J. D. Molkentin, J. Silver, and T. Naveh-Many
Calcineurin A{beta} Is Central to the Expression of the Renal Type II Na/Pi Co-transporter Gene and to the Regulation of Renal Phosphate Transport
J. Am. Soc. Nephrol., December 1, 2004; 15(12): 2972 - 2980.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
H. Segawa, I. Kaneko, S. Yamanaka, M. Ito, M. Kuwahata, Y. Inoue, S. Kato, and K.-i. Miyamoto
Intestinal Na-Pi cotransporter adaptation to dietary Pi content in vitamin D receptor null mice
Am J Physiol Renal Physiol, July 1, 2004; 287(1): F39 - F47.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online