AJP - Renal Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 284: F1155-F1163, 2003. First published February 11, 2003; doi:10.1152/ajprenal.00140.2002
0363-6127/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/6/F1155    most recent
00140.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (19)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thongboonkerd, V.
Right arrow Articles by Arthur, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thongboonkerd, V.
Right arrow Articles by Arthur, J. M.
Vol. 284, Issue 6, F1155-F1163, June 2003

Sodium loading changes urinary protein excretion: a proteomic analysis

Visith Thongboonkerd1, Jon B. Klein1,2,3, William M. Pierce4, Anthony W. Jevans5, and John M. Arthur6,7

1 Core Proteomics Laboratory, Kidney Disease Program, Department of Medicine, and Departments of 2 Biochemistry and Molecular Biology and 4 Pharmacology and Toxicology, University of Louisville, 3 Veterans Affairs Medical Center, and 5 Pathology Department, Jewish Hospital, Louisville, Kentucky 40202; and 6 Division of Nephrology, Department of Medicine, Medical University of South Carolina, and 7 Ralph H. Johnson Veterans Affairs Medical Center, Charleston, South Carolina 29425

Plasma sodium concentration is maintained even when sodium intake is altered. Sodium homeostasis may involve changes in renal tubular protein expression that are reflected in the urine. We used proteomic analysis to investigate changes in urinary protein excretion in response to acute sodium loading. Rats were given deionized water followed by hypertonic (2.7%) saline for 28 h each. Urinary protein expression was determined during the final 4 h of each treatment. Acute sodium loading increased urinary sodium excretion (4.53 ± 1.74 vs. 1.70 ± 0.27 mmol/day, P = 0.029). Urinary proteins were separated by two-dimensional PAGE and visualized by Sypro ruby staining. Differentially expressed proteins were identified by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry followed by peptide mass fingerprinting. The abundance of a total of 45 protein components was changed after acute sodium loading. Neutral endopeptidase, solute carrier family 3, meprin 1alpha , diphor-1, chaperone heat shock protein 72, vacuolar H+-ATPase, ezrin, ezrin/radixin/moesin-binding protein, glutamine synthetase, guanine nucleotide-binding protein, Rho GDI-1, and chloride intracellular channel protein 1 were decreased, whereas albumin and alpha -2u globulin were increased. Some of these proteins have previously been shown to be associated with tubular transport. These data indicate that alterations in the excretion of several urinary proteins occur during acute sodium loading.

urine; tubular transport


This article has been cited by other articles:


Home page
 Nephrol Dial TransplantHome page
V. Thongboonkerd
Current status of renal and urinary proteomics: ready for routine clinical application?
Nephrol. Dial. Transplant., September 16, 2009; (2009) gfp476v1.
[Full Text] [PDF]

Home page
 QJMHome page
K. Bramham, H.D. Mistry, L. Poston, L.C. Chappell, and A.J. Thompson
The non-invasive biopsy--will urinary proteomics make the renal tissue biopsy redundant?
QJM, August 1, 2009; 102(8): 523 - 538.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. G. Janech, J. R. Raymond, and J. M. Arthur
Proteomics in renal research
Am J Physiol Renal Physiol, February 1, 2007; 292(2): F501 - F512.
[Abstract] [Full Text] [PDF]

Home page
 Journal of Renin-Angiotensin-Aldosterone SystemHome page
P. K. Jacobsen
Review: Preventing End-Stage Renal Disease in Diabetic Patients -- Dual Blockade of the Renin-Angiotensin System (Part II)
Journal of Renin-Angiotensin-Aldosterone System, June 1, 2005; 6(2): 55 - 68.
[Abstract] [PDF]

Home page
 PhysiologyHome page
P. Cutillas, A. Burlingame, and R. Unwin
Proteomic Strategies and Their Application in Studies of Renal Function
Physiology, June 1, 2004; 19(3): 114 - 119.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.