AJP - Renal Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 285: F68-F78, 2003. First published March 25, 2003; doi:10.1152/ajprenal.00032.2003
0363-6127/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
285/1/F68    most recent
00032.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (19)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bergeron, M. J.
Right arrow Articles by Isenring, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bergeron, M. J.
Right arrow Articles by Isenring, P.

Ammonium transport and pH regulation by K+-Cl- cotransporters

Marc J. Bergeron,1 Édith Gagnon,1 Bernadette Wallendorff,2 Jean-Yves Lapointe,2 and Paul Isenring1

1Nephrology Group, Department of Medicine, Faculty of Medicine, Université Laval, Québec G1R 2J6; and 2Groupe de Recherche en Transport Membranaire, Physics Department, Université de Montréal, Montréal, Québec, Canada H3C 3J7

Submitted 22 January 2003 ; accepted in final form 18 March 2003

The Na+-K+-Cl- cotransporters (NKCCs), which belong to the cation-Cl- cotransporter (CCC) family, are able to translocate across cell membranes. In this study, we have used the oocyte expression system to determine whether the K+-Cl- cotransporters (KCCs) can also transport and whether they play a role in pH regulation. Our results demonstrate that all of the CCCs examined (NKCC1, NKCC2, KCC1, KCC3, and KCC4) can promote translocation, presumably through binding of the ion at the K+ site. Moreover, kinetic studies for both NKCCs and KCCs suggest that is an excellent surrogate of Rb+ or K+ and that transport and cellular acidification resulting from CCC activity are relevant physiologically. In this study, we have also found that CCCs are strongly and differentially affected by changes in intracellular pH (independently of intracellular []). Indeed, NKCC2, KCC1, KCC2, and KCC3 are inhibited at intracellular pH <7.5, whereas KCC4 is activated. These results indicate that certain CCC isoforms may be specialized to operate in acidic environments. CCC-mediated transport could bear great physiological implication given the ubiquitous distribution of these carriers.

NKCC; KCC; ion affinity; acidification; collecting duct


Address for reprint requests and other correspondence: P. Isenring, L'Hôtel-Dieu de Québec Research Ctr., 10 rue McMahon (Rm. 3852), Québec, Canada G1R2J6 (E-mail: paul.isenring{at}crhdq.ulaval.ca).




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
C. F. Simard, M. J. Bergeron, R. Frenette-Cotton, G. A. Carpentier, M.-E. Pelchat, L. Caron, and P. Isenring
Homooligomeric and Heterooligomeric Associations between K+-Cl- Cotransporter Isoforms and between K+-Cl- and Na+-K+-Cl- Cotransporters
J. Biol. Chem., June 22, 2007; 282(25): 18083 - 18093.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
B. Yang, D. Zhao, E. Solenov, and A. S. Verkman
Evidence from knockout mice against physiologically significant aquaporin 8-facilitated ammonia transport
Am J Physiol Cell Physiol, September 1, 2006; 291(3): C417 - C423.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. E. Handlogten, S.-P. Hong, L. Zhang, A. W. Vander, M. L. Steinbaum, M. Campbell-Thompson, and I. D. Weiner
Expression of the ammonia transporter proteins Rh B glycoprotein and Rh C glycoprotein in the intestinal tract
Am J Physiol Gastrointest Liver Physiol, May 1, 2005; 288(5): G1036 - G1047.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
G. Gamba
Molecular Physiology and Pathophysiology of Electroneutral Cation-Chloride Cotransporters
Physiol Rev, April 1, 2005; 85(2): 423 - 493.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. R. Williams and J. A. Payne
Cation transport by the neuronal K+-Cl- cotransporter KCC2: thermodynamics and kinetics of alternate transport modes
Am J Physiol Cell Physiol, October 1, 2004; 287(4): C919 - C931.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. E. Handlogten, S.-P. Hong, C. M. Westhoff, and I. D. Weiner
Basolateral ammonium transport by the mouse inner medullary collecting duct cell (mIMCD-3)
Am J Physiol Renal Physiol, October 1, 2004; 287(4): F628 - F638.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Gagnon, M. J. Bergeron, G. M. Brunet, N. D. Daigle, C. F. Simard, and P. Isenring
Molecular Mechanisms of Cl- Transport by the Renal Na+-K+-Cl- Cotransporter: IDENTIFICATION OF AN INTRACELLULAR LOCUS THAT MAY FORM PART OF A HIGH AFFINITY Cl--BINDING SITE
J. Biol. Chem., February 13, 2004; 279(7): 5648 - 5654.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
X. Liu, S. Titz, A. Lewen, and U. Misgeld
KCC2 Mediates NH4+ Uptake in Cultured Rat Brain Neurons
J Neurophysiol, October 1, 2003; 90(4): 2785 - 2790.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.