AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 285: F430-F439, 2003. First published May 20, 2003; doi:10.1152/ajprenal.00081.2002
0363-6127/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
285/3/F430    most recent
00081.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via ISI Web of Science (6)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hentschel, H.
Right arrow Articles by Elger, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hentschel, H.
Right arrow Articles by Elger, M.

Localization of Mg2+-sensing shark kidney calcium receptor SKCaR in kidney of spiny dogfish, Squalus acanthias

Hartmut Hentschel,1,2 Jacqueline Nearing,3 H. William Harris,3 Marlies Betka,3 Michelle Baum,4 Steven C. Hebert,5 and Marlies Elger2,6

1Max Planck Institute for Molecular Physiology, D-44229 Dortmund; 3MariCal, Incorporated, Portland 04101; 4Children's Hospital, Boston, Massachusetts 02115; 5Yale University School of Medicine, New Haven, Connecticut 06520; 6Department of Nephrology, Hannover Medical School, 30625 Hannover, Germany; and 2Mount Desert Island Biological Laboratory, Salsbury Cove, Maine 04672

Submitted 26 February 2002 ; accepted in final form 13 May 2003

We recently cloned a homologue of the bovine parathyroid calcium receptor from the kidney of a spiny dogfish (Squalus acanthias) and termed this new protein SKCaR. SKCaR senses alterations in extracellular Mg2+ after its expression in human embryonic kidney cells (Nearing J, Betka M, Quinn S, Hentschel H, Elger M, Baum M, Bai M, Chattopadyhay N, Brown E, Hebert S, and Harris HW. Proc Natl Acad. Sci USA 99: 9231-9236, 2002). In this report, we used light and electron microscopic immunocytochemical techniques to study the distribution of SKCaR in dogfish kidney. SKCaR antiserum bound to the apical membranes of shark kidney epithelial cells in the following tubular segments: proximal tubules (PIa and PIIb), late distal tubule, and collecting tubule/collecting duct as well as diffusely labeled cells of early distal tubule. The highly specific distribution of SKCaR in mesial tissue as well as lateral countercurrent bundles of dogfish kidney is compatible with a role for SKCaR to sense local tubular Mg2+ concentrations. This highly specific distribution of SKCaR protein in dogfish kidney could possibly work in concert with the powerful Mg2+ secretory system present in the PIIa segment of elasmobranch fish kidney to affect recycling of Mg2+ from putative Mg2+-sensing/Mg2+-reabsorbing segments. These data provide support for the possible existence of Mg2+ cycling in elasmobranch kidney in a manner analogous to that described for mammals.

renal handling of magnesium; transmembrane receptor protein; immunohistochemistry


Address for reprint requests and other correspondence: H. Hentschel, Max Planck Institute for Molecular Physiology, Otto-Hahn-Str.11, Postfach 500247, D-44229 Dortmund, Germany (E-mail: hartmut.hentschel{at}mpi-dortmund.mpg.de).






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.