|
|
||||||||
Departments of 1Internal Medicine, 6Physiology, and 3Neurobiology and Anatomy, University of Utah Health Sciences Center, Salt Lake City 84132; 2Nephrology Research and 5Geriatric Research, Education, and Clinical Center, Veterans Affairs Salt Lake City Health Care System, Salt Lake City, Utah 84148; and 5Department of Obstetrics and Gynecology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267
Submitted 15 March 2003 ; accepted in final form 15 May 2003
Extracellular nucleotides, acting through the P2Y2 receptor and the associated phosphoinositide-Ca2+ signaling pathway, inhibit AVP-stimulated osmotic water permeability in rat inner medullary collecting duct (IMCD). Because a rise in intracellular Ca2+ is frequently associated with enhanced arachidonic acid metabolism, we examined the effect of activation of the P2Y2 receptor on release of PGE2 in freshly prepared rat IMCD suspensions. Unstimulated IMCD released moderate, but significant, amounts of PGE2, which were more sensitive to cyclooxygenase (COX)-2 than COX-1 inhibition. Agonist activation of P2Y2 receptor by adenosine 5'-O-(3-thiotriphosphate) enhanced release of PGE2 from IMCD in a time- and concentration-dependent fashion. Purinergic-stimulated release of PGE2 was completely blocked by nonspecific COX inhibitors (flurbiprofen and 2-acetoxyphenylhept-2-ynyl sulfide). Differential COX inhibition studies revealed that purinergic-stimulated release of PGE2 was more sensitive to a COX-1-specific inhibitor (valeroyl salicylate) than a COX-2-specific inhibitor (NS-398). Thus purinergic stimulation resulted in significantly more release of PGE2 in the presence of COX-2 inhibitor than COX-1 inhibitor. If it is assumed that increased release of PGE2 is related to its increased production, our results suggest that purinergic stimulation of IMCD results in enhanced production and release of PGE2 in a COX-1-dependent fashion. Because PGE2 is known to affect transport of water, salt, and urea in IMCD, interaction of the purinergic system with the prostanoid system in IMCD can modulate handling of water, salt, and urea by IMCD and, thus, may constitute an AVP-independent regulatory mechanism.
cyclooxygenases; arachidonic acid; extracellular nucleotides; arginine vasopressin; purinergic receptor
This article has been cited by other articles:
![]() |
E. Degagne, D. M. Grbic, A.-A. Dupuis, E. G. Lavoie, C. Langlois, N. Jain, G. A. Weisman, J. Sevigny, and F.-P. Gendron P2Y2 Receptor Transcription Is Increased by NF-{kappa}B and Stimulates Cyclooxygenase-2 Expression and PGE2 Released by Intestinal Epithelial Cells J. Immunol., October 1, 2009; 183(7): 4521 - 4529. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. S. P. Wildman, M. Boone, C. M. Peppiatt-Wildman, A. Contreras-Sanz, B. F. King, D. G. Shirley, P. M. T. Deen, and R. J. Unwin Nucleotides Downregulate Aquaporin 2 via Activation of Apical P2 Receptors J. Am. Soc. Nephrol., July 1, 2009; 20(7): 1480 - 1490. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. Zhang, R. D. Nelson, N. G. Carlson, C. D. Kamerath, D. E. Kohan, and B. K. Kishore Potential role of purinergic signaling in lithium-induced nephrogenic diabetes insipidus Am J Physiol Renal Physiol, May 1, 2009; 296(5): F1194 - F1201. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Rieg and V. Vallon ATP and adenosine in the local regulation of water transport and homeostasis by the kidney Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2009; 296(2): R419 - R427. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. Zhang, J. M. Sands, D. E. Kohan, R. D. Nelson, C. F. Martin, N. G. Carlson, C. D. Kamerath, Y. Ge, J. D. Klein, and B. K. Kishore Potential role of purinergic signaling in urinary concentration in inner medulla: insights from P2Y2 receptor gene knockout mice Am J Physiol Renal Physiol, December 1, 2008; 295(6): F1715 - F1724. [Abstract] [Full Text] [PDF] |
||||
![]() |
V. Vallon P2 receptors in the regulation of renal transport mechanisms Am J Physiol Renal Physiol, January 1, 2008; 294(1): F10 - F27. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. Ge, K. A. Strait, P. K. Stricklett, T. Yang, and D. E. Kohan Role of prostaglandins in collecting duct-derived endothelin-1 regulation of blood pressure and water excretion Am J Physiol Renal Physiol, December 1, 2007; 293(6): F1805 - F1810. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Rieg, R. A. Bundey, Y. Chen, G. Deschenes, W. Junger, P. A. Insel, and V. Vallon Mice lacking P2Y2 receptors have salt-resistant hypertension and facilitated renal Na+ and water reabsorption FASEB J, November 1, 2007; 21(13): 3717 - 3726. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Shimamoto, Y. Nakanishi, K.-i. Katsu, T. Nakano, T. Kubota, H. Mori, and T. Nakahari Prostaglandin E2 release in gastric antral mucosa of guinea-pigs: basal PGE2 release by cyclo-oxygenase 2 and ACh-stimulated PGE2 release by cyclo-oxygenase 1 Exp Physiol, November 1, 2006; 91(6): 1015 - 1024. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. K. Hughes, P. K. Stricklett, B. K. Kishore, and D. E. Kohan Adenosine triphosphate inhibits endothelin-1 production by rat inner medullary collecting duct cells. Experimental Biology and Medicine, June 1, 2006; 231(6): 1006 - 1009. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. Nandigama, M. Padmasekar, M. Wartenberg, and H. Sauer Feed Forward Cycle of Hypotonic Stress-induced ATP Release, Purinergic Receptor Activation, and Growth Stimulation of Prostate Cancer Cells J. Biol. Chem., March 3, 2006; 281(9): 5686 - 5693. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. Sun, R. L. Miller, A. C. Hemmert, P. Zhang, H. Shi, R. D. Nelson, and B. K. Kishore Chronic dDAVP infusion in rats decreases the expression of P2Y2 receptor in inner medulla and P2Y2 receptor-mediated PGE2 release by IMCD Am J Physiol Renal Physiol, October 1, 2005; 289(4): F768 - F776. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. Sun, N. G. Carlson, A. C. Hemmert, and B. K. Kishore P2Y2 receptor-mediated release of prostaglandin E2 by IMCD is altered in hydrated and dehydrated rats: relevance to AVP-independent regulation of IMCD function Am J Physiol Renal Physiol, September 1, 2005; 289(3): F585 - F592. [Abstract] [Full Text] [PDF] |
||||
![]() |
B. K. Kishore, C. M. Krane, R. L. Miller, H. Shi, P. Zhang, A. Hemmert, R. Sun, and R. D. Nelson P2Y2 receptor mRNA and protein expression is altered in inner medullas of hydrated and dehydrated rats: relevance to AVP-independent regulation of IMCD function Am J Physiol Renal Physiol, June 1, 2005; 288(6): F1164 - F1172. [Abstract] [Full Text] [PDF] |
||||
![]() |
B. K. Kishore, J. Isaac, M. Fausther, S. R. Tripp, H. Shi, P. S. Gill, N. Braun, H. Zimmermann, J. Sevigny, and S. C. Robson Expression of NTPDase1 and NTPDase2 in murine kidney: relevance to regulation of P2 receptor signaling Am J Physiol Renal Physiol, May 1, 2005; 288(5): F1032 - F1043. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Shimamoto, S. Fujiwara, M. Kato, S. Ito, K.-i. Katsu, H. Mori, and T. Nakahari Inhibition of ACh-stimulated exocytosis by NSAIDs in guinea pig antral mucous cells: autocrine regulation of mucin secretion by PGE2 Am J Physiol Gastrointest Liver Physiol, January 1, 2005; 288(1): G39 - G47. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |