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Effect of flow and stretch on the [Ca²⁺]_i response of principal and intercalated cells in cortical collecting duct

¹Department of Pediatrics, Mount Sinai School of Medicine, New York 10029-6574; and²Department of Mechanical Engineering and Center for Biomedical Engineering, The City College of New York, New York, New York 10031

Submitted 19 February 2003 ; accepted in final form 25 June 2003

An acute increase in tubular fluid flow rate in the microperfused cortical collecting duct (CCD), associated with a 20% increase in tubular diameter, leads to an increase in intracellular Ca²⁺ concentration ([Ca²⁺]_i)in both principal and intercalated cells (Woda CB, Leite M Jr, Rohatgi R, and Satlin LM. Am J Physiol Renal Physiol 283: F437-F446, 2002). The apical cilium present in principal but not intercalated cells has been proposed to be a flow sensor. To determine whether flow across the cilium and/or epithelial stretch mediates the [Ca²⁺]_i response, CCDs from New Zealand White rabbits were microperfused in vitro, split-open (to isolate the effect of flow across cilia), or occluded (to examine the effect of stretch and duration/magnitude of the flow impulse), and [Ca²⁺]_i was measured using fura 2. In perfused and occluded CCDs, a rapid (<1 s) but not slow (>3 min) increase in luminal flow rate and/or circumferential stretch led to an approximately threefold increase in [Ca²⁺]_i in both principal and intercalated cells within 10 s. This response was mediated by external Ca²⁺ entry and inositol 1,4,5-trisphosphate-mediated release of cell Ca²⁺ stores. In split-open CCDs, an increase in superfusate flow led to an approximately twofold increase in [Ca²⁺]_i in both cell types within 30 s. These experimental findings are interpreted using mathematical models to predict the fluid stress on the apical membranes of the CCD and the forces and torques on and deformation of the cilia. We conclude that rapid increases in luminal flow rate and circumferential stretch, leading to shear or hydrodynamic impulses at the cilium or apical membrane, lead to increases in [Ca²⁺]_i in both principal and intercalated cells.

cilium; cytoskeletal deformation; fluid shear stress; mechanotransduction; fura 2; intracellular calcium concentration

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