| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of 1Medicine and 2Cell and Molecular Physiology, University of North Carolina, Chapel Hill, North Carolina 27599-7545
Submitted 24 March 2003 ; accepted in final form 30 July 2003
Tight junctions (TJ) regulate paracellular ionic charge selectivity and conductance across epithelial tissues and cell lines. These properties vary among epithelia, and recent evidence implicates the claudins, a family of TJ transmembrane proteins, as important determinants of both characteristics. To test the hypothesis that each claudin contributes a characteristic charge discrimination to the TJ, we expressed claudins-2, -4, -11, and -15 in both cation-selective Madin-Darby canine kidney (MDCK) II cells and in anion-selective LLC-PK1 cells and examined changes in transepithelial electrical resistance (TER) and paracellular charge selectivity. Regulated expression and localization were verified by immunoblot analysis and immunofluorescence microscopy, respectively. Expression of claudin-4 increased TER in both cell lines, whereas effects of the others on TER were variable. Claudin-4 and -11 decreased paracellular permeability for Na+ in MDCK II cells, whereas neither claudin-2 nor -15 had an effect. Conversely, in LLC-PK1 cells, claudin-2 and -15 increased the permeability for Na+, whereas claudin-4 and -11 were without effect. We conclude that the contribution of each claudin is most easily detectable when it reverses the direction of monolayer charge selectivity. These results are consistent with a model in which exogenous claudins add new charge-selective pores, leading to a physiological phenotype that combines endogenous and exogenous contributions. Additionally, it is possible to rationalize the direction of charge selectivity conferred by the individual claudins on the basis of electrostatic effects of the charged amino acids in their first extracellular loops.
tight junctions; dilution potential; intercellular junctions; Madin-Darby canine kidney; LLC-PK1
This article has been cited by other articles:
|
|
 |
|
  B. E. Phillips, L. Cancel, J. M. Tarbell, and D. A. Antonetti Occludin Independently Regulates Permeability under Hydrostatic Pressure and Cell Division in Retinal Pigment Epithelial Cells Invest. Ophthalmol. Vis. Sci., June 1, 2008; 49(6): 2568 - 2576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. K. Tipsmark, P. Kiilerich, T. O. Nilsen, L. O. E. Ebbesson, S. O. Stefansson, and S. S. Madsen Branchial expression patterns of claudin isoforms in Atlantic salmon during seawater acclimation and smoltification Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2008; 294(5): R1563 - R1574. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Mrsny, G. T. Brown, K. Gerner-Smidt, A. G. Buret, J. B. Meddings, C. Quan, M. Koval, and A. Nusrat A Key Claudin Extracellular Loop Domain is Critical for Epithelial Barrier Integrity Am. J. Pathol., April 1, 2008; 172(4): 905 - 915. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Van Itallie, J. Holmes, A. Bridges, J. L. Gookin, M. R. Coccaro, W. Proctor, O. R. Colegio, and J. M. Anderson The density of small tight junction pores varies among cell types and is increased by expression of claudin-2 J. Cell Sci., February 1, 2008; 121(3): 298 - 305. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Piontek, L. Winkler, H. Wolburg, S. L. Muller, N. Zuleger, C. Piehl, B. Wiesner, G. Krause, and I. E. Blasig Formation of tight junction: determinants of homophilic interaction between classic claudins FASEB J, January 1, 2008; 22(1): 146 - 158. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Jovov, C. M. Van Itallie, N. J. Shaheen, J. L. Carson, T. M. Gambling, J. M. Anderson, and R. C. Orlando Claudin-18: a dominant tight junction protein in Barrett's esophagus and likely contributor to its acid resistance Am J Physiol Gastrointest Liver Physiol, December 1, 2007; 293(6): G1106 - G1113. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. B. Singh, K. Sugimoto, P. Dhawan, and R. C. Harris Juxtacrine activation of EGFR regulates claudin expression and increases transepithelial resistance Am J Physiol Cell Physiol, November 1, 2007; 293(5): C1660 - C1668. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Hermo, N. Korah, M. Gregory, L. Y. Liu, D. G. Cyr, A. D'Azzo, and C. E. Smith Structural Alterations of Epididymal Epithelial Cells in Cathepsin A Deficient Mice Affect the Blood-Epididymal Barrier and Lead to Altered Sperm Motility J Androl, September 1, 2007; 28(5): 784 - 797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Skrovanek, M. C. Valenzano, and J. M. Mullin Restriction of sulfur-containing amino acids alters claudin composition and improves tight junction barrier function Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2007; 293(3): R1046 - R1055. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Angelow, R. El-Husseini, S. A. Kanzawa, and A. S. L. Yu Renal localization and function of the tight junction protein, claudin-19 Am J Physiol Renal Physiol, July 1, 2007; 293(1): F166 - F177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Dube, P. T.K. Chan, L. Hermo, and D. G. Cyr Gene Expression Profiling and Its Relevance to the Blood-Epididymal Barrier in the Human Epididymis Biol Reprod, June 1, 2007; 76(6): 1034 - 1044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Flores-Benitez, A. Ruiz-Cabrera, C. Flores-Maldonado, L. Shoshani, M. Cereijido, and R. G. Contreras Control of tight junctional sealing: role of epidermal growth factor Am J Physiol Renal Physiol, February 1, 2007; 292(2): F828 - F836. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C R Weber and J R Turner Inflammatory bowel disease: is it really just another break in the wall? Gut, January 1, 2007; 56(1): 6 - 8. [Full Text] [PDF]
|
|
|
|
|
|
S Zeissig, N Burgel, D Gunzel, J Richter, J Mankertz, U Wahnschaffe, A J Kroesen, M Zeitz, M Fromm, and J-D Schulzke Changes in expression and distribution of claudin 2, 5 and 8 lead to discontinuous tight junctions and barrier dysfunction in active Crohn's disease Gut, January 1, 2007; 56(1): 61 - 72. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Abuazza, A. Becker, S. S. Williams, S. Chakravarty, H.-T. Truong, F. Lin, and M. Baum Claudins 6, 9, and 13 are developmentally expressed renal tight junction proteins Am J Physiol Renal Physiol, December 1, 2006; 291(6): F1132 - F1141. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Van Itallie, S. Rogan, A. Yu, L. S. Vidal, J. Holmes, and J. M. Anderson Two splice variants of claudin-10 in the kidney create paracellular pores with different ion selectivities Am J Physiol Renal Physiol, December 1, 2006; 291(6): F1288 - F1299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Gonzalez-Mariscal, M. Del CarmenNamorado, D. Martin, G. Sierra, and J. L. Reyes The tight junction proteins claudin-7 and -8 display a different subcellular localization at Henle's loops and collecting ducts of rabbit kidney Nephrol. Dial. Transplant., September 1, 2006; 21(9): 2391 - 2398. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Arabzadeh, T.-C. Troy, and K. Turksen Role of the Cldn6 Cytoplasmic Tail Domain in Membrane Targeting and Epidermal Differentiation In Vivo Mol. Cell. Biol., August 1, 2006; 26(15): 5876 - 5887. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ikari, S. Matsumoto, H. Harada, K. Takagi, H. Hayashi, Y. Suzuki, M. Degawa, and M. Miwa Phosphorylation of paracellin-1 at Ser217 by protein kinase A is essential for localization in tight junctions J. Cell Sci., May 1, 2006; 119(9): 1781 - 1789. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. F. Balkovetz Claudins at the gate: determinants of renal epithelial tight junction paracellular permeability Am J Physiol Renal Physiol, March 1, 2006; 290(3): F572 - F579. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Angelow, K.-J. Kim, and A. S. L. Yu Claudin-8 modulates paracellular permeability to acidic and basic ions in MDCK II cells J. Physiol., February 15, 2006; 571(1): 15 - 26. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Le Moellic, S. Boulkroun, D. Gonzalez-Nunez, I. Dublineau, F. Cluzeaud, M. Fay, M. Blot-Chabaud, and N. Farman Aldosterone and tight junctions: modulation of claudin-4 phosphorylation in renal collecting duct cells Am J Physiol Cell Physiol, December 1, 2005; 289(6): C1513 - C1521. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Baum and R. Quigley Maturation of rat proximal tubule chloride permeability Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2005; 289(6): R1659 - R1664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hou, D. L. Paul, and D. A. Goodenough Paracellin-1 and the modulation of ion selectivity of tight junctions J. Cell Sci., November 1, 2005; 118(21): 5109 - 5118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Alexandre, Q. Lu, and Y.-H. Chen Overexpression of claudin-7 decreases the paracellular Cl- conductance and increases the paracellular Na+ conductance in LLC-PK1 cells J. Cell Sci., June 15, 2005; 118(12): 2683 - 2693. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Van Itallie, T. M. Gambling, J. L. Carson, and J. M. Anderson Palmitoylation of claudins is required for efficient tight-junction localization J. Cell Sci., April 1, 2005; 118(7): 1427 - 1436. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. P. Chen, B. Zhou, B. C. Willis, A. J. Sandoval, J. M. Liebler, K.-J. Kim, D. K. Ann, E. D. Crandall, and Z. Borok Effects of transdifferentiation and EGF on claudin isoform expression in alveolar epithelial cells J Appl Physiol, January 1, 2005; 98(1): 322 - 328. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Boitano, Z. Safdar, D. G. Welsh, J. Bhattacharya, and M. Koval Cell-cell interactions in regulating lung function Am J Physiol Lung Cell Mol Physiol, September 1, 2004; 287(3): L455 - L459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Rahner, M. Fukuhara, S. Peng, S. Kojima, and L. J. Rizzolo The apical and basal environments of the retinal pigment epithelium regulate the maturation of tight junctions during development J. Cell Sci., July 1, 2004; 117(15): 3307 - 3318. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
