| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Program in Membrane Biology and Renal Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114
Submitted 8 May 2003 ; accepted in final form 24 September 2003
Inhibition of clathrin-mediated endocytosis by expression of a GTPase-deficient dynamin mutant (dynamin-2/K44A) for 16 h results in an accumulation of plasma membrane aquaporin-2 (AQP2) in epithelial cells stably transfected with wild-type AQP2. We now show a similar effect of K44A dynamin in LLC-PK1 cells transfected with an S256 phosphorylation-deficient AQP2 mutant, AQP2(S256A), and in AQP2-transfected inner medullary collecting duct (IMCD) cells. More acute blockade of endocytosis in these cells with the cholesterol-depleting agent methyl-
-cyclodextrin (mCD; 10 mM) resulted in a rapid and extensive cell-surface accumulation of both wild-type AQP2 and AQP2 (S256A) within 15 min after treatment. This effect was similar to that induced by treatment of the cells with vasopressin. Blockade of endocytosis by mCD was confirmed using quantitative analysis of FITC-dextran uptake and AQP2 membrane insertion was verified by cell-surface biotinylation. These data indicate that AQP2 recycles constitutively and rapidly between intracellular stores and the cell surface in LLC-PK1 and IMCD cells. The constitutive trafficking process is not dependent on phosphorylation of the serine-256 residue of AQP2, which is, however, an essential step for regulated vasopressin/cAMP-mediated translocation of AQP2. Our data show that rapid and extensive plasma membrane accumulation of AQP2 can occur in a vasopressin receptor (V2R)- and phosphorylation-independent manner, pointing to a potential means of bypassing the mutated V2R in X-linked nephrogenic diabetes insipidus to achieve cell surface expression of AQP2.
inner medullary collecting duct; aquaporin-2; methyl--cyclodextrin
This article has been cited by other articles:
|
|
 |
|
  Y. Noda, S. Horikawa, E. Kanda, M. Yamashita, H. Meng, K. Eto, Y. Li, M. Kuwahara, K. Hirai, C. Pack, et al. Reciprocal interaction with G-actin and tropomyosin is essential for aquaporin-2 trafficking J. Cell Biol., August 11, 2008; 182(3): 587 - 601. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. J. Lu, T. Matsuzaki, R. Bouley, U. Hasler, Q.-H. Qin, and D. Brown The phosphorylation state of serine 256 is dominant over that of serine 261 in the regulation of AQP2 trafficking in renal epithelial cells Am J Physiol Renal Physiol, July 1, 2008; 295(1): F290 - F294. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Fenton, H. B. Moeller, J. D. Hoffert, M.-J. Yu, S. Nielsen, and M. A. Knepper Acute regulation of aquaporin-2 phosphorylation at Ser-264 by vasopressin PNAS, February 26, 2008; 105(8): 3134 - 3139. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. M. Pastor-Soler, K. R. Hallows, C. Smolak, F. Gong, D. Brown, and S. Breton Alkaline pH- and cAMP-induced V-ATPase membrane accumulation is mediated by protein kinase A in epididymal clear cells Am J Physiol Cell Physiol, February 1, 2008; 294(2): C488 - C494. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Goel, W. G. Sinkins, C.-D. Zuo, U. Hopfer, and W. P. Schilling Vasopressin-induced membrane trafficking of TRPC3 and AQP2 channels in cells of the rat renal collecting duct Am J Physiol Renal Physiol, November 1, 2007; 293(5): F1476 - F1488. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Stefan, B. Wiesner, G. S. Baillie, R. Mollajew, V. Henn, D. Lorenz, J. Furkert, K. Santamaria, P. Nedvetsky, C. Hundsrucker, et al. Compartmentalization of cAMP-Dependent Signaling by Phosphodiesterase-4D Is Involved in the Regulation of Vasopressin-Mediated Water Reabsorption in Renal Principal Cells J. Am. Soc. Nephrol., January 1, 2007; 18(1): 199 - 212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Klein, O. Frohlich, M. A. Blount, C. F. Martin, T. D. Smith, and J. M. Sands Vasopressin Increases Plasma Membrane Accumulation of Urea Transporter UT-A1 in Rat Inner Medullary Collecting Ducts J. Am. Soc. Nephrol., October 1, 2006; 17(10): 2680 - 2686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Russo, M. McKee, and D. Brown Methyl-beta-cyclodextrin induces vasopressin-independent apical accumulation of aquaporin-2 in the isolated, perfused rat kidney Am J Physiol Renal Physiol, July 1, 2006; 291(1): F246 - F253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Procino, D. B. Caces, G. Valenti, and J. E. Pessin Adipocytes support cAMP-dependent translocation of aquaporin-2 from intracellular sites distinct from the insulin-responsive GLUT4 storage compartment Am J Physiol Renal Physiol, May 1, 2006; 290(5): F985 - F994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Bouley, N. Pastor-Soler, O. Cohen, M. McLaughlin, S. Breton, and D. Brown Stimulation of AQP2 membrane insertion in renal epithelial cells in vitro and in vivo by the cGMP phosphodiesterase inhibitor sildenafil citrate (Viagra) Am J Physiol Renal Physiol, June 1, 2005; 288(6): F1103 - F1112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Swiatecka-Urban, C. Boyd, B. Coutermarsh, K. H. Karlson, R. Barnaby, L. Aschenbrenner, G. M. Langford, T. Hasson, and B. A. Stanton Myosin VI Regulates Endocytosis of the Cystic Fibrosis Transmembrane Conductance Regulator J. Biol. Chem., September 3, 2004; 279(36): 38025 - 38031. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
