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Intestinal Na-P_i cotransporter adaptation to dietary P_i content in vitamin D receptor null mice

¹Nutritional Science, Department of Nutrition, School of Medicine, Tokushima University, Tokushima City 770-8503; and ²Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo 113-0032, Japan

Submitted 27 October 2003 ; accepted in final form 25 February 2004

Recent studies suggest that vitamin D may play a role in intestinal Na⁺-dependent phosphate transport adaptation to variable levels of dietary P_i. Therefore, the goal of the current study was to assess Na⁺-dependent P_i cotransport activity in transgenic mice to determine whether vitamin D is an essential mediator of this process. Intestinal brush-border membrane (BBM), Na⁺-dependent P_i cotransport activity was significantly decreased in vitamin D receptor (VDR) null [VDR (–/–)] mice compared with wild-type (VDR+/+) mice. While intestinal Na-P_i cotransporter (type IIb) mRNA levels were similar in VDR (–/–) and VDR (+/+) mice, type IIb Na-P_i cotransporter protein expression was markedly suppressed in VDR (–/–) mice compared with VDR (+/+) mice. Furthermore, Na-P_i cotransport activity in renal BBM was similar in VDR (–/–) and VDR (+/+) mice, but type IIa Na-P_i cotransporter protein expression was decreased in VDR (–/–) mice. After administration of a low-P_i diet, type IIb protein expression was significantly increased in VDR (+/+) and VDR (–/–) mice, and type IIb protein expression was present in the intestinal BBM of VDR (–/–) mice. These data demonstrate that intestinal Na-P_i cotransport adaptation to a low-P_i diet occurs independently of vitamin D.

dietary phosphate; phosphate transport; intestine

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