HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 287/2/F188    most recent 00421.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Jin, J.-S. Articles by Chen, A. Search for Related Content PubMed PubMed Citation Articles by Jin, J.-S. Articles by Chen, A.

Adriamycin impairs the contraction of mesangial cells through the inhibition of protein kinase C and intracellular calcium

¹Department of Pathology, Tri-Service General Hospital, and ²Department of Biochemistry, National Defense Medical Center, Taipei 114, Taiwan, Republic of China

Submitted 2 December 2003 ; accepted in final form 21 April 2004

The effects of adriamycin on the contractile function of cultured mesangial cells were measured by the changes in planar surface area in response to treatment with agonists. Incubation of mesangial cells with adriamycin (0.2 µg/ml) for 24 h significantly decreased the contractile responses to the calcium channel activator BAY K 8644 (1 µM) and to the PKC activator PMA (1 µM). Intracellular calcium concentration ([Ca²⁺]_i), measured by changes in fura 2 levels in response to ATP (0.1 mM), was significantly inhibited in adriamycin-treated mesangial cells compared with control cells. In the absence of extracellular calcium, treatment with ionomycin (0.1 mM) or thapsigargin (10 µM) resulted in a significantly smaller increase in [Ca²⁺]_i in adriamycin-treated mesangial cells compared with control, suggesting an important role of the endoplasmic reticulum in the effects of adriamycin. Using PKC-specific antibodies, adriamycin significantly decreased the cytosolic and membranous fractions of PKC- in mesangial cells to 75 ± 6 and 70 ± 12% of control, respectively. The PKC activity of mesangial cells was also significantly inhibited after incubation with adriamycin for 24 h. In conclusion, adriamycin induces hypocontractility of mesangial cells, which may mediate this effect by inhibiting PKC- and [Ca²⁺]_i.

planar surface area; protein kinase C-; protein kinase C activity; fura 2

This article has been cited by other articles:

				T.-H. Huang, H.-A. Shui, S.-M. Ka, B.-L. Tang, T.-K. Chao, J.-S. Chen, Y.-F. Lin, and A. Chen Rab 23 is expressed in the glomerulus and plays a role in the development of focal segmental glomerulosclerosis Nephrol. Dial. Transplant., March 1, 2009; 24(3): 743 - 754. [Abstract] [Full Text] [PDF]

				B. Miller, V. A. Patel, and A. Sorokin Cyclooxygenase-2 Rescues Rat Mesangial Cells from Apoptosis Induced by Adriamycin via Upregulation of Multidrug Resistance Protein 1 (P-Glycoprotein) J. Am. Soc. Nephrol., April 1, 2006; 17(4): 977 - 985. [Abstract] [Full Text] [PDF]