HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 287/2/F289    most recent 00273.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (12) Citing Articles via Google Scholar Google Scholar Articles by Redling, S. Articles by Vallon, V. Search for Related Content PubMed PubMed Citation Articles by Redling, S. Articles by Vallon, V.

Immunolocalization of protein kinase C isoenzymes , I, II, , and in mouse kidney

¹Institute of Pharmacology and Toxicology, University of Tübingen, 72074 Tübingen; ²Max-Planck-Institute for Experimental Endocrinology, 30625 Hannover, Germany; and ³Departments of Medicine and Pharmacology, University of California, and Veterans Affairs Medical Center, San Diego, California 92161

Submitted 7 August 2003 ; accepted in final form 22 March 2004

Localization of protein kinase C (PKC) isoenzymes , I, II, , and was studied employing Western blot analysis and immunohistochemical methods including confocal laser-scanning microscopy in the kidney of two mice strains, namely, C57BL/6 and 129/Sv, which have recently been used as genetic backgrounds for respective knockout mice. Immunoblot analysis identified immunoreactive bands for each isoenzyme in total kidney cell extracts. Isoenzyme expression sites were identical for both strains. Glomeruli expressed PKC-, -I, and -. The latter isoenzme was also detected in apical aspects of proximal convoluted but not in proximal straight tubules. In contrast to rats, neither PKC- nor PKC-I was detectable in the proximal tubule. Immunofluorescence was observed in luminal membranes of medullary (MTAL) and cortical thick ascending limbs for PKC-I and in MTAL for PKC-. The cortical collecting duct expressed PKC-, -I, and - in intercalated cells only. In the outer medullary collecting duct, PKC- and -I were detectable in principal cells, whereas PKC- was found in intercalated cells. In the inner medullary collecting duct, PKC-, -I, and -II were detected. As described for the rat, the expression of PKC-II was otherwise restricted to cortical and medullary interstitial cells. The specificity of all labeling was confirmed in respective PKC isoenzyme knockout mice. In summary, distinct expression patterns were shown for PKC isoenzymes , I, II, , and in the mouse kidney.

immunohistochemistry; knockout; renal interstitial cells

This article has been cited by other articles:

				W. Liu, Y. Wei, P. Sun, W.-H. Wang, T. R. Kleyman, and L. M. Satlin Mechanoregulation of BK channel activity in the mammalian cortical collecting duct: role of protein kinases A and C Am J Physiol Renal Physiol, October 1, 2009; 297(4): F904 - F915. [Abstract] [Full Text] [PDF]

				D. Wu, F. Peng, B. Zhang, A. J. Ingram, D. J. Kelly, R. E. Gilbert, B. Gao, and J. C. Krepinsky PKC-{beta}1 Mediates Glucose-Induced Akt Activation and TGF-{beta}1 Upregulation in Mesangial Cells J. Am. Soc. Nephrol., March 1, 2009; 20(3): 554 - 566. [Abstract] [Full Text] [PDF]

				R. A. Fenton and M. A. Knepper Mouse Models and the Urinary Concentrating Mechanism in the New Millennium Physiol Rev, October 1, 2007; 87(4): 1083 - 1112. [Abstract] [Full Text] [PDF]

				F. Rothenberger, A. Velic, P. A. Stehberger, J. Kovacikova, and C. A. Wagner Angiotensin II Stimulates Vacuolar H+-ATPase Activity in Renal Acid-Secretory Intercalated Cells from the Outer Medullary Collecting Duct J. Am. Soc. Nephrol., July 1, 2007; 18(7): 2085 - 2093. [Abstract] [Full Text] [PDF]

				M. Meier, J.-K. Park, D. Overheu, T. Kirsch, C. Lindschau, F. Gueler, M. Leitges, J. Menne, and H. Haller Deletion of Protein Kinase C-{beta} Isoform In Vivo Reduces Renal Hypertrophy but Not Albuminuria in the Streptozotocin-Induced Diabetic Mouse Model Diabetes, February 1, 2007; 56(2): 346 - 354. [Abstract] [Full Text] [PDF]

				W.-Y. Kim, J.-H. Jung, E.-Y. Park, C.-W. Yang, H. Kim, S. Nielsen, K. M. Madsen, and J. Kim Expression of protein kinase C isoenzymes {alpha}, betaI, and {delta} in subtypes of intercalated cells of mouse kidney Am J Physiol Renal Physiol, November 1, 2006; 291(5): F1052 - F1060. [Abstract] [Full Text] [PDF]

				V. Aaltonen, J. Koivunen, M. Laato, and J. Peltonen Heterogeneity of Cellular Proliferation within Transitional Cell Carcinoma: Correlation of Protein Kinase C Alpha/betaI Expression and Activity J. Histochem. Cytochem., July 1, 2006; 54(7): 795 - 806. [Abstract] [Full Text] [PDF]

				L. Yao, D.-Y. Huang, I. L. Pfaff, X. Nie, M. Leitges, and V. Vallon Evidence for a role of protein kinase C-{alpha} in urine concentration Am J Physiol Renal Physiol, August 1, 2004; 287(2): F299 - F304. [Abstract] [Full Text] [PDF]