HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 287/3/F373    most recent 00418.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (17) Citing Articles via Google Scholar Google Scholar Articles by Kumar, V. Articles by Lieske, J. C. Search for Related Content PubMed PubMed Citation Articles by Kumar, V. Articles by Lieske, J. C.

Renal epithelial cells constitutively produce a protein that blocks adhesion of crystals to their surface

¹Division of Nephrology, Department of Internal Medicine, Mayo Clinic College of Medicine, Rochester, Minnesota 55905; and ²Department of Medicine, University of Chicago, Chicago, Illinois 60637

Submitted 2 December 2003 ; accepted in final form 17 April 2004

Attachment of newly formed crystals to renal tubular epithelial cells appears to be a critical step in the development of kidney stones. The present study was undertaken to identify autocrine factors released from renal epithelial cells into the culture medium that inhibit adhesion of calcium oxalate crystals to the cell surface. A 39-kDa glycoprotein that is constitutively secreted by renal cells was purified by gel filtration chromatography. Amino acid microsequencing revealed that it is novel and not structurally related to known inhibitors of calcium oxalate crystallization. Hence, it was named crystal adhesion inhibitor, or CAI. Immunoreactive CAI was detected in diverse rat tissues, including kidney, heart, pancreas, liver, and testis. Immunohistochemistry revealed that CAI is present in the renal cell cytosol and is also on the plasma membrane. Importantly, CAI is present in normal human urine, from which it can be purified using calcium oxalate monohydrate crystal affinity chromatography. CAI could be an important defense against crystal attachment to tubular cells and the subsequent development of renal stones in vivo.

calcium oxalate monohydrate; cell-crystal interaction; DING protein; inhibitor; nephrolithiasis

This article has been cited by other articles:

				M. L. Merchant, T. D. Cummins, D. W. Wilkey, S. A. Salyer, D. W. Powell, J. B. Klein, and E. D. Lederer Proteomic analysis of renal calculi indicates an important role for inflammatory processes in calcium stone formation Am J Physiol Renal Physiol, October 1, 2008; 295(4): F1254 - F1258. [Abstract] [Full Text] [PDF]

				P. K. Grover, L. A. Thurgood, and R. L. Ryall Effect of urine fractionation on attachment of calcium oxalate crystals to renal epithelial cells: implications for studying renal calculogenesis Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1396 - F1403. [Abstract] [Full Text] [PDF]

				N. Stankus, E. Worcester, M. Hammes, and F. L. Coe Evidence against a contribution of conventional urine risk factors to de novo ESRD renal stones Nephrol. Dial. Transplant., March 1, 2006; 21(3): 701 - 706. [Abstract] [Full Text] [PDF]